[1]苗卉△,李娜,苗聪秀.左卡尼汀对玻璃化冷冻小鼠卵母细胞体外受精结局的影响[J].郑州大学学报(医学版),2015,(02):277.
 MIAO Hui,LI Na,MIAO Congxiu.Effects of Lcarnitine on in vitrofertilized outcomes of mouse oocytes underwent vitrification[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(02):277.
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左卡尼汀对玻璃化冷冻小鼠卵母细胞体外受精结局的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年02期
页码:
277
栏目:
应用研究
出版日期:
2015-03-20

文章信息/Info

Title:
Effects of Lcarnitine on in vitrofertilized outcomes of mouse oocytes underwent vitrification
作者:
苗卉△李娜苗聪秀
长治医学院附属和平医院生殖中心 长治 046000
Author(s):
MIAO HuiLI NaMIAO Congxiu
Center of Reproduction, the Affiliated Heping Hospital,Changzhi Medical College,Changzhi 046000
关键词:
玻璃化冷冻卵母细胞左卡尼汀
Keywords:
vitrificationoocyteLcarnitine
分类号:
Q813.7
摘要:
目的:研究左卡尼汀对玻璃化冷冻小鼠卵母细胞的影响。方法:将卵母细胞分为新鲜组(存活卵数243个),玻璃化冷冻组(解冻卵数282个),左卡尼汀高、中、低剂量(2.0、1.0、0.5 g/L)组(解冻卵数分别为285、280和279个)。观察各组卵母细胞中GSH含量、ROS水平以及卵母细胞存活率、受精率、卵裂率、囊胚形成率。结果:5组卵母细胞GSH含量、ROS水平及卵母细胞存活率、受精率、卵裂率、囊胚形成率差异均有统计学意义(F=881.290、182.760及χ2=113.361、21.560、16.660、9.667,P均<0.05)。与新鲜卵母细胞比较,玻璃化冷冻卵母细胞中GSH含量降低,ROS水平升高,卵母细胞存活率、受精率、卵裂率均降低(P<0.05)。左卡尼汀呈剂量依赖性地增加玻璃化冷冻卵母细胞中GSH含量,降低ROS水平(P<0.05);高剂量左卡尼汀可明显改善玻璃化冷冻卵母细胞的存活率(P<0.05)。结论:左卡尼汀可通过抗氧化作用改善玻璃化冷冻卵母细胞的结局。
Abstract:
Aim: To study the effects of Lcarnitine on the in vitrofertilized outcomes of mouse oocytes underwent vitrification. Methods: Oocytes were divided into fresh group(243 survival oocytes), vitrification group(282 thawed oocytes), Lcarnitine high, medium and low dose (2.0, 1.0, 0.5 g/L) groups(thawed oocytes number was 285,280,279,respectively). The GSH content, ROS level in oocytes and survival rate, fertilization rate, cleavage rate, blastocyst formation rate of oocytes in each group were observed. Results: There were significant differences in the GSH content,ROS level in the oocytes and the survival rate, fertilization rate, cleavage rate, and blastocyst formation rate among the 5 groups(F=881.290,182.760, and χ2=113.361,21.560,16.660,9.667,P<0.05).Compared with those of the fresh group, the GSH content was lower,ROS level was higher in the oocytes of vitrification group, and the survival rate, fertilization rate, and cleavage rate decreased(P<0.05). Lcarnitine could increase the GSH content, reduce ROS level in the oocytes underwent vitrification, and high dose Lcarnitine could improve the survival rate(P<0.05). Conclusion: Lcarnitine may improve the outcomes of oocytes which undergo vitrification through antioxidant effects.

参考文献/References:

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更新日期/Last Update: 1900-01-01