[1]高巍,宋平义,景桂霞,等.丙泊酚对HepG2肝母细胞瘤细胞侵袭能力的影响*[J].郑州大学学报(医学版),2015,(03):318-322.
 GAO Wei,SONG Pingyi,JING Guixia,et al.Effects of propofol on the invasion ability of hepatoblastoma HepG2 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(03):318-322.
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丙泊酚对HepG2肝母细胞瘤细胞侵袭能力的影响*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年03期
页码:
318-322
栏目:
论著
出版日期:
2015-05-20

文章信息/Info

Title:
Effects of propofol on the invasion ability of hepatoblastoma HepG2 cells
作者:
高巍1宋平义1景桂霞1张晓琪1南克俊2沙保勇3
1西安交通大学医学部第一附属医院麻醉科 西安 7100612西安交通大学医学部第一附属医院肿瘤内科 西安 710061 3西安医学院基础医学部基础医学研究所 西安 710021
Author(s):
GAO Wei1 SONG Pingyi1 JING Guixia1 ZHANG Xiaoqi1 NAN Kejun2 SHA Baoyong3
1Department of Anesthesiology, the First Affiliated Hospital, Xi′an Jiaotong University, Xi′an 7100612Department of Oncology, the First Affiliated Hospital, Xi′an Jiaotong University, Xi′an 7100613Institute of Basic Medical Sciences, Xi′an Medical University, Xi′an 710021
关键词:
丙泊酚肝母细胞瘤MMP2MMP9TIMP1侵袭
Keywords:
propofolhepatoblastomaMMP2MMP9TIMP1invasion
分类号:
R735.7
文献标志码:
A
摘要:
目的:探讨丙泊酚对HepG2肝母细胞瘤细胞侵袭能力的影响及相关机制。方法:用不同质量浓度(0、3、6、9和12 mg/L)丙泊酚培养HepG2细胞24 h后,通过细胞侵袭实验检测侵袭率;培养24、48和72 h后用MTT法检测细胞活性;培养48 h后采用RTPCR和Western blot法检测细胞中MMP2、MMP9 mRNA及MMP2、MMP9和TIMP1蛋白的表达。结果:随丙泊酚质量浓度的增加,HepG2细胞的侵袭能力逐渐降低(F=4 883.900,P<0.001),细胞活性逐渐降低(F=38.334,P<0.001),MMP2及MMP9 mRNA及蛋白的表达亦逐渐降低(P<0.05),而TIMP1蛋白的表达逐渐升高(F=44.918,P<0.001)。结论:丙泊酚能降低HepG2细胞的侵袭能力及细胞活性,其机制与抑制MMP2和MMP9蛋白的表达及促进TIMP1蛋白的表达有关。
Abstract:
To investigate the effects and related mechanism of propofol on the invasion ability of HepG2 cells. Methods: Different concentrations(0,3,6,9 and 12 mg/L) of propofol were used to treat HepG2 cells. The invasion ability was detected by Transwell after 24 h treatment; cell viability of HepG2 cells was studied through MTT assay after 24,48,and 72 h treatment. The expressions of MMP2,MMP9 and TIMP1 were measured through RTPCR and Western blot after 48 h treatment. Results: The invasion ability and cell viability of HepG2 cells, the mRNA and protein expressions of MMP2 and MMP9, were significantly decreased with the increase of propofol concentration(P<0.05). The protein expression of TIMP1 was significantly increased with the increase of propofol concentration(F=44.918,P<0.001). Conclusion: Propofol could reduce the invasive activity and cell viability of HepG2 cells through the inhibition of MMP2 and MMP9, and overexpression of TIMP1.

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备注/Memo

备注/Memo:
通信作者:沙保勇,男,1982年3月生,博士,讲师,研究方向:中枢神经功能损伤与重建,Email:shabaoyong@gmail.com;景桂霞,女,1958年9月生,教授,研究方向:麻醉药物对肿瘤的影响,Email:jgx666@126.com
更新日期/Last Update: 1900-01-01