[1]常悦,王维嘉,朴军颜,等.KYBNZ1I对Tca8113 细胞增殖的抑制作用[J].郑州大学学报(医学版),2015,(03):413-415.
 CHANG Yue,WANG Weijia,PIAO Junyan,et al.Inhibitive effect of KYBNZ1I on proliferation of Tca8113 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(03):413-415.
点击复制

KYBNZ1I对Tca8113 细胞增殖的抑制作用()
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年03期
页码:
413-415
栏目:
应用研究
出版日期:
2015-05-20

文章信息/Info

Title:
Inhibitive effect of KYBNZ1I on proliferation of Tca8113 cells
作者:
常悦1王维嘉2朴军颜3姚珂3左非非3徐少博3徐霞3
1郑州大学口腔医学院正畸科 郑州 4500522郑州大学临床医学系 郑州 4500013郑州大学药学院 郑州 450001
Author(s):
CHANG Yue1WANG Weijia2PIAO Junyan3YAO Ke3ZUO Feifei3XU Shaobo3XU Xia3
1Departmemt of Orthodontics,College of Stomatology,Zhengzhou University,Zhengzhou 4500522Department of Clinical Medicine, Zhengzhou University,Zhengzhou 4500013College of Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 450001
关键词:
KYBNZ1ITca8113细胞增殖凋亡细胞周期
Keywords:
KYBNZ1ITca8113 cellproliferationapoptosiscell cycle
分类号:
R780.2
文献标志码:
A
摘要:
目的:观察冬凌草提取物KYBNZ1I对口腔鳞状细胞癌细胞Tca8113增殖的抑制作用,并探讨其可能机制。方法:采用MTT法检测0.5、1.0、2.0、4.0、8.0 mg/L的KYBNZ1I分别作用24、48、72 h对Tca8113细胞增殖的抑制作用。分别采用流式细胞仪及Western blot检测0.0、1.0、2.0、3.0 mg/L的KYBNZ1I作用48 h Tca8113细胞的周期分布、凋亡情况及CyclinD1、CyclinB1、Cdc2蛋白的表达。结果:KYBNZ1I对Tca8113细胞增殖有抑制作用,该作用呈剂量和时间依赖性(F剂量=133.991,F时间=22.526,P<0.05)。与对照组比较,1.0、2.0、3.0 mg/L的KYBNZ1I作用48 h后G2/M期细胞增多,并可诱导细胞凋亡,呈剂量依赖性(F=108.465、212.353,P<0.05);随药物剂量的升高,CyclinD1蛋白的表达水平下降(F=45.288,P<0.001),CyclinB1、Cdc2蛋白的表达水平升高(F=21.273、19.228,P<0.05)。结论:KYBNZ1I对Tca8113细胞增殖有抑制作用,可将细胞阻滞于G2/M期并诱导细胞凋亡,其作用机制可能与KYBNZ1I抑制CyclinD1蛋白的表达、提高CyclinB1和Cdc2蛋白的表达有关。
Abstract:
To investigate the effect of KYBNZ1I on proliferation of oral squamous cell carcinoma cell line Tca8113 and the possible mechanism.Methods: The effect of KYBNZ1I(0.5,1.0,2.0,4.0 and 8.0 mg/L) on proliferation of Tca8113 cells for 24,48 and 72 h was measured by MTT assay. Tca8113 cells were treated by 0.0,1.0,2.0 and 3.0 mg/L KYBNZ1I for 48 h, then flow cytometry and Western blot were used to detect the cell cycle distribution, apoptosis and the expressions of CyclinD1,CyclinB1 and Cdc2.Results: KYBNZ1I inhibited the proliferation of Tca8113 cells in a time and dose dependent manner(Fdose=133.991,Ftime=22.526,P<0.05). Compared with control group, the percentage of G2/M phase Tca8113 cells was significantly higher(F=108.465,P<0.001),and cell apoptosis rate raised(F=212.353,P<0.001) in a dosedependent manner. With the increase of KYBNZ1I concentration, the expression of CyclinD1 decreased(F=45.288,P<0.001),and the expressions of CyclinB1 and Cdc2 increased(F=21.273 and 19.228,P<0.05).Conclusion: KYBNZ1I could inhibit proliferation, arrest the cells at G2/M phase and induce cell apoptosis, which may be explained by its restraining expression of CyclinD1, and upregulating expressions of CyclinB1 and Cdc2.

参考文献/References:

[1]张超贤,郭李柯,史淑敏.饮酒和细胞外超氧化物岐化酶、乙醛脱氢酶2基因多态性与口腔鳞状细胞癌的相关性研究[J].华西口腔医学杂志,2014,32(2):119$
[2]张逸凡,关忠民,陈笑艳,等.抗肿瘤新药埃克替尼在动物体内的药动学和组织分布研究[J].中国新药杂志,2014,23(2):235$
[3]Tan F,Shen X,Wang D,et al.Icotinib(BPI2009H), a novel EGFR tyrosine kinase inhibitor, displays potent efficacy in preclinical studies[J].Lung Cancer,2012,76(2):177$
[4]Liu Z,Ouyang L,Peng H,et al.Oridonin:targeting programmed cell death pathways as an antitumour agent[J].Cell Prolif,2012,45(6):499$
[5]尹波,李志伟,张弩,等.冬凌草甲素对C6大鼠脑胶质瘤裸鼠异种移植模型的抗肿瘤疗效研究[J].肿瘤学杂志,2014,20(1):34$
[6]王绍丰,卢啟立.冬凌草的现代研究概况[J].广州医药,2008,39(1):46$
[7]黄涛,张令达.口腔鳞状细胞癌组织中p57kip2和Cyclin D1 mRNA的表达及意义[J].安徽医科大学学报,2013,48(4):398$
[8]Yunlan L,Juan Z,Qingshan L.Antitumor activity of dinbutyl(2,6difluorobenzohydroxamato)tin(Ⅳ) against human gastric carcinoma SGC7901 cells via G2/M cell cycle arrest and cell apoptosis[J].PLoS One,2014,9(3):e90793$
[9]Hunter T,Pines J.Cyclins and cancer.Ⅱ:Cyclin D and CDK inhibitors come of age[J].Cell,1994,79(4):573$
[10]Yuan SY,Lin CC,Hsu SL,et al.Leaf extracts of calocedrus formosana(Florin) induce G2/M cell cycle arrest and apoptosis in human bladder cancer cells[J].Evid Based Complement Alternat Med,2011(2011):380923

相似文献/References:

[1]吴大鹏)△,姜睿斌),赵德强),等.β榄香烯乳对体外培养Tca8113细胞放射增敏、细胞周期、凋亡及Bcl2、Bax蛋白表达的影响[J].郑州大学学报(医学版),2009,(02):414.
 WU Dapeng),JIANG Ruibin),ZHAO Deqiang),et al.Effects of βelemene on radiosensitivity, cell cycle, apoptosis, Bcl2 and Bax expression of Tca8113 cells in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):414.
[2]韩德昌)),梁建锋),韩新光)#.LY294002对舌鳞状细胞癌细胞增殖及PTEN蛋白表达的影响[J].郑州大学学报(医学版),2009,(03):613.
 HAN Dechang),LIANG Jianfeng),HAN Xinguang).Effect of LY294002 on human tongue squamous carcinoma cell proliferation and PTEN protein expression in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):613.
[3]吴大鹏△,卢红,张洪志.β榄香烯乳联合照射对人舌鳞状细胞癌裸鼠移植瘤的影响[J].郑州大学学报(医学版),2010,(03):474.
 WU Dapeng,LU Hong,ZHANG Hongzhi.Effects of βelemene combined with radiotherapy on human squamous cell carcinoma of tongue transplanted model in nude mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(03):474.

备注/Memo

备注/Memo:
通信作者,女,1965年4月生,博士研究生,教授,研究方向:药物代谢,Email:xuxia@zzu.edu.cn
更新日期/Last Update: 1900-01-01