[1]杨露,张楠楠,张彦婷,等.Ets2 siRNA转染对EC9706细胞增殖、侵袭、周期和凋亡的影响[J].郑州大学学报(医学版),2015,(04):453-457.
 YANG Lu,ZHANG Nannan,ZHANG Yanting,et al.Effect of Ets2 siRNA transfection on proliferation,invasion,cell cycle and apoptosis of EC9706 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(04):453-457.
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Ets2 siRNA转染对EC9706细胞增殖、侵袭、周期和凋亡的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年04期
页码:
453-457
栏目:
食管癌研究
出版日期:
2015-07-20

文章信息/Info

Title:
Effect of Ets2 siRNA transfection on proliferation,invasion,cell cycle and apoptosis of EC9706 cells
作者:
杨露1张楠楠2张彦婷1李庆华1许尧1朱相展1薛乐勋1#关方霞12#
1)郑州大学生命科学学院 郑州 4500012)郑州大学第一附属医院干细胞研究室 郑州 450052
Author(s):
YANG Lu1 ZHANG Nannan2 ZHANG Yanting1 LI Qinghua1 XU Yao1 ZHU Xiangzhan1 XUE Lexun1 GUAN Fangxia12
1)College of Life Sciences,Zhengzhou University,Zhengzhou 4500012)Stem Cell Laboratory,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
Ets2增殖侵袭细胞周期凋亡EC9706细胞
Keywords:
Ets2proliferationinvasioncell cycleapoptosisEC9706 cell
分类号:
R735.1
文献标志码:
A
摘要:
目的:探讨Ets2 siRNA转染对EC9706细胞增殖、侵袭能力、周期和凋亡的影响。方法:利用Western blot检测正常食管上皮细胞系Het1A和食管鳞状细胞癌细胞系(EC1、Eca109、EC9706)中Ets2蛋白的表达。将EC9706细胞分为4组:阴性对照组、空白对照组、转染试剂对照组和Ets2 siRNA组,处理48 h后,采用EdU荧光标记法和CCK8法检测4组细胞增殖率和增殖抑制率,Transwell小室法检测细胞侵袭能力,Western blot检测Ecadherin、Caspase3和Bcl2蛋白的表达情况,流式细胞仪检测细胞周期,Annexin VFITC/PI双染法检测早期细胞凋亡。结果:与Het1A 细胞相比,EC1、Eca109和EC9706细胞中Ets2蛋白的表达量增加(F=1 177.764,P<0.001),且EC9706细胞中增加最为显著。转染后,与阴性对照组相比,Ets2 siRNA组细胞增殖率降低、增殖抑制率升高(F=64.733和144.741,P<0.05),侵袭能力减弱(F=104.065,P<0.001),细胞周期无明显变化(P>0.05),早期凋亡细胞增加(F=37.986,P<0.001),Ecadherin蛋白和Caspase3蛋白表达增加(F=62.223和81.015,P<0.05),而Bcl2蛋白表达减少(F=104.439,P<0.001)。结论:Ets2下调后能有效抑制EC9706细胞增殖,降低侵袭能力,诱导凋亡;Ets2可能成为食管鳞状细胞癌的有效治疗靶点。
Abstract:
Aim: To investigate the effect of siRNAmediated downregulation of Ets2 on the proliferation, invasion, cell cycle and apoptosis of EC9706 cells in vitro. Methods: The expression of Ets2 in Het1A, EC1, Eca109,and EC9706 cells was analyzed by Western blot. EC9706 cells were allocated into 4 groups: negative control group, blank control group, siRNA control group, and Ets2 siRNA group. The proliferation of EC9706 cells was examined by EdU method and CCK8 method; the invasion was analyzed by Transwell; cell cycle was studied by flow cytometry; apoptosis was analyzed by Annexin VFITC/PI flow cytometry and the protein expressions of Ecadherin,Caspase3 and Bcl2 were detected by Western blot.Results: Compared with Het1A cells, Ets2 showed higher expression in EC1,Eca109,and EC9706 cells(F=1 177.764,P<0.001), especially in EC9706 cells. Compared with negative control group,the proliferation rate of EC9706 cells decreased and the inhibition rate increased significantly(F=64.733,144.741,P<0.05). The number of invasion cells was lower in Ets2 siRNA group than that of the negative control group(F=104.065,P<0.001). The cell cycle among the 4 groups had no obvious change(P>0.05). Compared with the negative control group , Ets2 siRNA group had a higher apoptosis rate(F=37.986,P<0.001). The protein expression levels of Ecadherin and Caspase3 increased significantly(F=62.223,81.015,P<0.05) and the Bcl2 protein expression level decreased obviously(F=104.439,P<0.001). Conclusion: siRNAmediated downregulation of Ets2 could effectively inhibit the proliferation of EC9706 cells,decrease the invasion and induce the apoptosis. Therefore, Ets2 may be a potential therapeutic target for ESCC.

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