[1]许林平,张瑞芳,王鹏#,等.肿瘤相关抗原组合在肿瘤诊断中的价值[J].郑州大学学报(医学版),2015,(04):466-468.
 XU Linping,ZHANG Ruifang,WANG Peng,et al.Value of multiple tumorassociated antigens arrays in diagnosis of cancer[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(04):466-468.
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肿瘤相关抗原组合在肿瘤诊断中的价值()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年04期
页码:
466-468
栏目:
论著
出版日期:
2015-07-20

文章信息/Info

Title:
Value of multiple tumorassociated antigens arrays in diagnosis of cancer
作者:
许林平1张瑞芳2 王鹏3#张建营3
1)郑州大学附属肿瘤医院;河南省肿瘤医院科研外事办 郑州 4500082)新乡医学院公共卫生学院实验室管理办公室 新乡4530033)郑州大学公共卫生学院流行病学教研室;河南省肿瘤流行病学重点实验室 郑州 450001
Author(s):
XU Linping1 ZHANG Ruifang2 WANG Peng3 ZHANG Jianying3
1)Department of Scientific Research and Foreign Affairs, the Affiliated Cancer Hospital, Zhengzhou University; Henan Cancer Hospital, Zhengzhou 4500082)Lab Administration Office, School of Public Health, Xinxiang Medical University, Xinxiang 4530033)Department of Epidemiology, College of Public Health; Henan Key Laboratory of Tumor Epidemiology, Zhengzhou University, Zhengzhou 450001
关键词:
肿瘤相关抗原抗原组合诊断效能
Keywords:
tumorassociated antigenantigen arraydiagnosis value
分类号:
R730.4
文献标志码:
A
摘要:
目的:评价多种肿瘤相关抗原(TAA)组合在肿瘤诊断中的价值。方法:应用ELISA法检测304份不同类型肿瘤(98例肺癌、69例食管癌、50例肝癌、46例结直肠癌、41例乳癌)患者和58份正常人血清中8种TAA(Koc、p62、IMP1、cyclinE、p16、survivin、cmyc、p53)的自身抗体,用蛋白印迹技术验证其免疫活性,评价用TAA诊断肿瘤的真实性、可靠性和收益。结果:肿瘤患者血清中8种TAA阳性率普遍偏低,随着TAA的增多直至8种TAA组合,对肿瘤的诊断灵敏度达到63.5%,特异度达86.2%,对不同类型肿瘤诊断的约登指数为0.423~0.522,阳性、阴性预测值为74.2%~88.7%、58.8%~75.8%,阳性、阴性似然比4.07~4.76、0.39~0.51,8种TAA诊断肿瘤的一致率和Kappa值分别为67.1%和0.51。结论:多种TAA组合对肿瘤具有较高的诊断价值。
Abstract:
Aim: To evaluate the diagnostic value of multiple tumorassociated antigens(TAAs) in different types of cancer. Methods: Eight TAAs including Koc, p62, IMP1, cyclinE, p16, survivin, cmyc and p53 in sera from 304 cancer patients (98 cases of lung cancer,69 cases of esophageal cancer,50 cases of liver cancer,46 cases of colorectal cancer and 41 cases of breast cancer) and 58 normal individuals were detected by ELISA. Positive results of ELISA were confirmed by Western blot. Results: The positive rate of the 8 TAAs in different cancer patients were generally low. With the successive addition of TAA to a final total of eight antigens, there was a stepwise increase of positive reactions reaching to a sensitivity of 63.5% and a specificity of 86.2%. In different cancer, the Youden′s index was 0.423-0.522, positive and negative predictive value was 74.2%-88.7% and 58.8%-75.8%, while positive and negative likelihood ratio was 4.07-4.76 and 0.39-0.51, which showed that the clinical diagnostic value of a assays of 8 TAAs was high. Agreement rate and Kappa value were 67.1% and 0.51, respectively. Conclusion: Multiple TAA arrays may have higher diagnostic value for tumor diagnosis.

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更新日期/Last Update: 1900-01-01