[1]张建波,吕晓东,宋巍,等.乳癌患者转移淋巴结中T细胞受体β链互补决定区3谱型分析[J].郑州大学学报(医学版),2015,(04):481-484.
 ZHANG Jianbo,LV Xiaodong,SONG Wei,et al.Analysis of complementarity determining region 3 repertoire of T cell receptor β chain in metastatic lymph node from patients with breast cancer[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(04):481-484.
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乳癌患者转移淋巴结中T细胞受体β链互补决定区3谱型分析()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年04期
页码:
481-484
栏目:
论著
出版日期:
2015-07-20

文章信息/Info

Title:
Analysis of complementarity determining region 3 repertoire of T cell receptor β chain in metastatic lymph node from patients with breast cancer
作者:
张建波1吕晓东2宋巍1孙淼淼1于庆凯1冯稳1刘明阁1胡骏3房百俊4#
1)郑州大学附属肿瘤医院病理科 郑州 4500032)郑州大学附属肿瘤医院中心实验室 郑州 4500033)中山大学中山医学院微生物学教研室 广州 5100894)郑州大学附属肿瘤医院血液科 郑州 450003
Author(s):
ZHANG Jianbo1 LV Xiaodong2SONG Wei1SUN Miaomiao1 YU Qingkai1FENG Wen1 LIU Mingge1HU Jun3FANG Baijun4
1)Department of Pathology, the Affiliated Cancer Hospital, Zhengzhou University,Zhengzhou 450003 2)Central Laboratory, the Affiliated Cancer Hospital, Zhengzhou University,Zhengzhou 450003 3)Department of Microbiology, Zhongshan Medical College,Sun Yatsen University, Guangzhou 5100894)Department of Hematology, the Affiliated Cancer Hospital, Zhengzhou University,Zhengzhou 450003
关键词:
乳腺肿瘤T细胞受体互补决定区3
Keywords:
breast neoplasmT cell receptorcomplementarity determining region 3
分类号:
R737.9
文献标志码:
A
摘要:
目的:分析乳癌患者转移淋巴结标本中T细胞受体(TCR)β链互补决定区3(CDR3)的谱型偏移及氨基酸序列特点。方法:选取5例乳癌患者转移淋巴结和2例乳腺反应性增生患者的淋巴结标本,应用RTPCR扩增T细胞24个TCR β链可变区(BV)亚家族,经基因扫描确定T细胞克隆性,对提示单、寡克隆性增生的TCR BV亚家族的PCR产物进行测序。结果:乳腺反应性增生患者淋巴结中T细胞均为多克隆性增生。乳癌患者转移淋巴结中T细胞呈单、寡克隆及多克隆性增生,克隆性增生T细胞中存在2~5个BV亚家族的谱型偏移;CDR3氨基酸序列分析证实单、寡克隆性增生T细胞的CDR3氨基酸序列呈现多样性的特点。结论:乳癌患者TCR BV亚家族取用存在谱型偏移,不同T细胞克隆的CDR3氨基酸序列不同。
Abstract:
Aim: To analyze the specific repertoire skewing and amino acid sequences of the complementarity determining region 3(CDR3) of T cell receptor(TCR) β chain in metastatic lymph node from patients with breast cancer. Methods: A total of 24 TCR β chain variable region(BV) subfamilies were amplified by RTPCR, and the CDR3 lengths were analyzed with GeneScan method in lymph nodes from 2 patients with reactive hyperplasia and metastatic lymph nodes from 5 patients with breast cancer.Sequence analysis of the CDR3 region in monoclonal or oligoclonal expanded T cells was performed.Results: There were only multiclonal expanded T cells in lymph nodes from patients with reactive hyperplasia, while there were monoclonal, oligoclonal, and multiclonal expanded T cells in metastatic lymph nodes from patients with breast cancer. TCR BV presented specific repertoire skewing in metastatic lymph node from patients with breast cancer, among which only 25 TCR BV subfamilies were identified.The monoclonal or oligoclonal expanded T cells had different CDR3 amino acid sequences. Conclusion: There is specific repertoire skewing of TCR BV subfamilies in metastatic lymph nodes from patients with breast cancer. The amino acid sequences of CDR3 in different T cell clone proliferation are mostly different.

参考文献/References:

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更新日期/Last Update: 1900-01-01