[1]牛敏,邵天波,陈瑞春,等.溃疡性结肠炎患者肠道菌群分析和细菌毒素基因检测[J].郑州大学学报(医学版),2015,(04):514-518.
 NIU Min,SHAO Tianbo,CHEN Ruichun,et al.R574.620.4[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(04):514-518.
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溃疡性结肠炎患者肠道菌群分析和细菌毒素基因检测()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年04期
页码:
514-518
栏目:
应用研究
出版日期:
2015-07-20

文章信息/Info

Title:
R574.620.4
作者:
牛敏邵天波陈瑞春杜艳#
昆明医科大学第一附属医院检验科 昆明 650000
Author(s):
NIU MinSHAO TianboCHEN RuichunDU Yan
Clinical Laboratory,the First Affiliated Hospital,Kunming Medical University,Kunming 650000
关键词:
溃疡性结肠炎菌群失调细菌毒素
Keywords:
ulcerative colitisdysbacteriosisintestinal toxin
分类号:
R574.620.4
文献标志码:
A
摘要:
目的:探讨溃疡性结肠炎(UC)患者肠道菌群分布和细菌毒素基因的检出情况。方法:收集UC活动期患者32例、缓解期患者21例和45名健康对照的新鲜粪便标本,采用直接涂片镜检和传统细菌培养鉴定法分析菌群分布,利用PCR检测粪便中6种常见的肠道致病菌毒素基因。结果:直接涂片镜检结果显示,UC活动期组菌群失调率(87.5%)高于对照组(53.3%,χ2=9.957,P=0.003),UC活动期组菌群失调程度高于缓解期组(Z=2.501,P=0.012)。需氧培养结果显示,UC组大肠埃希菌优势生长率(66.0%)低于对照组(88.9%,χ2=7.075,P=0.008),条件致病菌优势生长率(32.1%)明显高于对照组(11.1%,χ2=6.144, P=0.013),变形杆菌属是UC组优势生长率最高的条件致病菌。厌氧培养结果显示,UC组益生菌和条件致病菌优势生长率与对照组比较差异并无统计学意义(P>0.05)。UC组6种细菌毒素基因的总检出率(41.5%)明显高于对照组(22.2%,χ2=4.117, P=0.042)。结论:UC患者存在明显的肠道菌群失调。
Abstract:
Aim: To explore the intestinal flora distribution in ulcerative colitis(UC) patients and detect the bacterial toxin gene.Methods: Fresh feces samples were collected from 32 UC active patients, 21 UC inactive patients and 45 healthy persons. Firstly, the feces flora distribution was analyzed through smear gram stain and microscopy, and bacteria strain culture and identification by traditional method. Then 6 bacterial toxin genes were detected by PCR.Results: The dysbacteriosis rate in UC active group(87.5%) was significantly higher than that of the healthy group(53.3%,χ2=9.957,P=0.003). The dysbacteriosis degree of UC active group was higher than that of the inactive group(Z=2.501, P=0.012). The results of aerobic culture showed that the dominance rate of E.coli in UC group(66.0%) was significantly lower than that in the healthy group(88.9%,χ2=7.075,P=0.008). The dominance rate of conditional pathogenic bacteria in UC group(32.1%) was higher than that in healthy group(11.1%,χ2=6.144,P=0.013). Proteus spp was the most dominance conditional pathogenic bacteria in UC group. The results of anaerobic culture showed that the dominance rates of probiotics and conditional pathogenic bacteria in UC group and healthy group had no significant differences(P>0.05). The total detection rate of the 6 bacteria toxin gene in UC group(41.5%) was higher than that in healthy group(22.2%,χ2=4.117,P=0.042).Conclusion: There is intestinal dysbacteriosis in patients with UC.

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更新日期/Last Update: 1900-01-01