[1]崔智慧,崔岩,孙高洁,等.利拉鲁肽对小鼠脂肪细胞脂肪因子、炎症因子及其信号通路的影响[J].郑州大学学报(医学版),2015,(04):519-521.
 CUI Zhihui,CUI Yan,SUN Gaojie,et al.Effect of liraglutide on the adipokine,inflammatory factors and inflammatory signaling pathway in the mouse adipocytes[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(04):519-521.
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利拉鲁肽对小鼠脂肪细胞脂肪因子、炎症因子及其信号通路的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年04期
页码:
519-521
栏目:
应用研究
出版日期:
2015-07-20

文章信息/Info

Title:
Effect of liraglutide on the adipokine,inflammatory factors and inflammatory signaling pathway in the mouse adipocytes
作者:
崔智慧崔岩孙高洁王姣王守俊#
郑州大学第一附属医院内分泌科 郑州 450052
Author(s):
CUI ZhihuiCUI YanSUN GaojieWANG JiaoWANG Shoujun
Department of Endocrinology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
利拉鲁肽3T3L1细胞瘦素脂联素CTRP3TNFαIL6IKKβ
Keywords:
liraglutide3T3L1 cellleptinadpionectinCTRP3TNFαIL6IKKβ
分类号:
R587.1
文献标志码:
A
摘要:
目的:观察利拉鲁肽对小鼠3T3L1脂肪细胞脂肪因子、炎症因子及其信号通路的影响。方法:不同浓度(0、1、10和100 nmol/L)利拉鲁肽作用于诱导分化完全的3T3L1脂肪细胞48 h后,qRTPCR法检测瘦素、脂联素、CTRP3、TNFα、IL6 mRNA的表达情况;100 nmol/L的利拉鲁肽作用于诱导分化完全的3T3L1脂肪细胞0、8、24、48 h后,qRTPCR法检测瘦素、脂联素、CTRP3、TNFα、IL6 mRNA的表达情况;Western blot法检测不同浓度(0、1、10、100 nmol/L)利拉鲁肽作用于诱导分化完全的3T3L1脂肪细胞48 h后,细胞IKKβ蛋白的表达及其磷酸化(ser181)水平。结果:随着利拉鲁肽浓度的增加,3T3L1脂肪细胞内的瘦素、TNFα、IL6的mRNA表达水平逐渐降低(F=62.613、61.382和86.610,P<0.001);而脂联素和CTRP3的mRNA表达水平逐渐升高(F=35.960和43.712,P<0.001)。100 nmol/L利拉鲁肽作用于3T3L1脂肪细胞0、8、24、48 h,细胞内的瘦素、TNFα、IL6的mRNA表达水平逐渐降低(F=51.641、134.632和45.185,P<0.001);而脂联素和CTRP3的mRNA表达水平逐渐升高(F=25.727和24.179,P<0.001)。0、1、10和100 nmol/L利拉鲁肽作用于3T3L1脂肪细胞48 h后,IKKβ的磷酸化水平逐渐降低。结论:利拉鲁肽可能通过抑制IKKβ参与的炎症信号通路改善胰岛素抵抗。
Abstract:
Aim: To explore the effect of liraglutide on the adipokine,inflammatory factors and inflammatory signaling pathway in the mouse adipocytes.Methods: The fully differentiated adipocytes(3T3L1 cells) were treated with liraglutide at different concentrations(0,1,10 and 100 nmol/L) for 48 h,then the expression levels of leptin,adpionectin,CTRP3,TNFα and IL6 mRNA were determined by qRTPCR;the fully differentiated adipocytes were treated with liraglutide at a concentration of 100 nmol/L for 0,8,24,48 h, then the expression levels of leptin,adpionectin,CTRP3,TNFα and IL6 mRNA were determined by qRTPCR; the fully differentiated adipocytes were treated with liraglutide at different concentrations(0,1,10 and 100 nmol/L) for 48 h,then the expressions of IKKβ and the phosphorylated IKKβ(ser181) were detected by Western blot.Results: Compared with control group,different concentrations of liraglutide reduced the expression levels of leptin,TNFα,and IL6 mRNA(F=62.613,61.382 and 86.610,P<0.001),but improved the expression levels of adpionectin and CTRP3 mRNA of 3T3L1 cells(F=35.960 and 43.712,P<0.001). Compared with control group,after being treated by 100 nmol/L liraglutide for 8,24,and 48 h,the expression levels of leptin,TNFα, and IL6 mRNA reduced(F=51.641,134.632 and 45.185,P<0.001),while those of adpionctin and CTRP3 mRNA increased(F=25.727,24.179,P<0.001).Different concentrations(0,1,10,100 nmol/L) of liraglutide reduced the phosphorylation of IKKβ(ser181) of 3T3L1 cells.Conclusion: Liraglutide may improve insulin resistance by inhibiting the inflammatory signaling pathways involved of IKKβ.

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更新日期/Last Update: 1900-01-01