[1]韩丽萍△,余海洋,朱科科,等.紫外线B照射对HeLa细胞中IEX-1-mRNA表达的影响[J].郑州大学学报(医学版),2015,(04):529-531.[doi:10.13705/j.issn.1671.6825.2015.04.021]
 HAN Liping,YU Haiyang,ZHU Keke,et al.Effect of ultraviolet B irradiation on expression of IEX1 mRNA in HeLa cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(04):529-531.[doi:10.13705/j.issn.1671.6825.2015.04.021]
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紫外线B照射对HeLa细胞中IEX-1-mRNA表达的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年04期
页码:
529-531
栏目:
应用研究
出版日期:
2015-07-20

文章信息/Info

Title:
Effect of ultraviolet B irradiation on expression of IEX1 mRNA in HeLa cells
作者:
韩丽萍12余海洋12朱科科12孙晓慧12张晓雪12
1)郑州大学第一附属医院妇产科 郑州 4500522)河南省高等学校临床医学重点学科开放实验室 郑州 450052
Author(s):
HAN Liping12YU Haiyang12ZHU Keke12SUN Xiaohui12ZHANG Xiaoxue12
1)Department of Obstetrics and Gynecology,the First Affiliated Hospital, Zhengzhou University,Zhengzhou 4500522)KeyDisciplines Laboratory of ClinicalMedicine of Henan, Zhengzhou 450052
关键词:
IEX1紫外线B照射宫颈癌HeLa细胞
Keywords:
IEX1ultraviolet B irradiationcervical cancerHeLa cell
DOI:
10.13705/j.issn.1671.6825.2015.04.021
文献标志码:
A
摘要:
目的:观察紫外线B(UVB)照射HeLa细胞后IEX1 mRNA表达的变化。方法:常规培养人宫颈癌HeLa细胞,采用RTPCR法检测剂量为0、2、4、8、16、32 mJ/cm2的UVB照射30 s后HeLa细胞中IEX1 mRNA 的表达情况,同法检测剂量为32 mJ/cm2的UVB照射后即刻及培养1、2、4、8、16 h时IEX1 mRNA 的表达情况。结果:随UVB剂量的升高,HeLa细胞IEX1 mRNA的表达量呈升高的趋势(F=644.725,P<0.001)。32 mJ/cm2的UVB照射HeLa细胞后即刻及培养1、2、4、8、16 h,IEX1 mRNA的表达呈升高的趋势(F=56.249,P<0.001),培养4 h后IEX1 mRNA表达水平达峰值。结论:HeLa细胞中IEX1 mRNA的表达可被UVB迅速、短暂地诱导,且具有剂量依赖性。
Abstract:
Aim: To detect the effect of ultraviolet B(UVB) irradiation on the expression of IEX1 mRNA in HeLa cells. Methods: HeLa cells were irradiated with 315 nmUVBray at doses of 0,2,4,8,16,32 mJ/cm2 for 30 s and then the level of IEX1 mRNA expression was detected by RTPCR. HeLa cells were irradiated with UVBray at the dose of 32 mJ/cm2 for 30 s and the level of IEX1 mRNA expression was detected by RTPCR after being cultured for 0,1,2,4,8,16 h,respectively. Results: After being irradiated by different doses of UVBray, the expression of IEX1 mRNA increased(F=644.725,P<0.001), especially at 32 mJ/cm2. After being irradiated by UVBray at the dose of 32 mJ/cm2, the ex

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