[1]王慧丽△,谢冰,李欣,等.人参皂苷Rd对高糖致人牙周膜干细胞成骨分化抑制作用的影响[J].郑州大学学报(医学版),2015,(04):542-545.
 WANG Huili,XIE Bing,LI Xin,et al.Effects of ginsenoside Rd on osteogenic differentiation of high glucoseinduced human periodontal ligament stem cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(04):542-545.
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人参皂苷Rd对高糖致人牙周膜干细胞成骨分化抑制作用的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年04期
页码:
542-545
栏目:
应用研究
出版日期:
2015-07-20

文章信息/Info

Title:
Effects of ginsenoside Rd on osteogenic differentiation of high glucoseinduced human periodontal ligament stem cells
作者:
王慧丽1谢冰1李欣1蔺燕萍1张朋2
1)郑州大学附属郑州中心医院口腔科 郑州 4500072)郑州大学口腔医学院修复科 郑州 450052
Author(s):
WANG Huili1XIE Bing1LI Xin1LIN Yanping1ZHANG Peng2
1)Department of Stomatology, the Affiliated Central Hospital of Zhengzhou, Zhengzhou University, Zhengzhou 4500072)Department of Prosthodontics, College of Stomatology, Zhengzhou University, Zhengzhou 450052
关键词:
人参皂苷Rd牙周膜干细胞成骨分化氧化应激
Keywords:
ginsenoside Rdperiodontal ligament stem cellosteogenic differentiationoxidative stress
分类号:
R781.4
文献标志码:
A
摘要:
目的:探讨高糖诱导条件对人牙周膜干细胞(PDLSCs)增殖及分化的影响,并探讨人参皂苷Rd(GSRd)对高糖诱导PDLSCs成骨分化的影响。方法:PDLSCs经高糖(30 mmol/L D葡萄糖)或对照(5 mmol/L D葡萄糖)处理后,通过测定PDLSCs的碱性磷酸酶(ALP)活性、骨钙蛋白(OCN)和ALP的mRNA表达及骨桥蛋白(OPN)的蛋白表达,以评估PDLSCs成骨分化水平。同时通过检测GSRd对PDLSCs经高糖处理后超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的mRNA及蛋白表达水平,以评估GSRd对高糖诱导PDLSCs成骨分化中氧化应激的影响。结果:高糖处理可减低PDLSCs中ALP的活性(P<0.05),而10和40 μmol/L 的GSRd可提高正常和高糖培养条件下PDLSCs中ALP的活性(P<0.05);并且,GSRd可上调ALP、OCN mRNA表达及OPN蛋白表达(F=102.893、56.061、60.296, P<0.05),上调CAT、SOD的mRNA及蛋白表达水平(F=48.393、67.134、29.837、7.539,P<0.05)。结论:GSRd对体外培养的PDLSCs的成骨分化有一定的促进作用;在高糖条件,GSRd可以减轻氧化应激对PDLSCs成骨的抑制作用,促进PDLSCs的成骨作用。
Abstract:
Aim: To explore the effects of ginsenoside Rd(GSRd) on osteogenic differentiation of high glucoseinduced human periodontal ligament stem cells(PDLSCs). Methods: PDLSCs were pretreated with 30 mmol/L Dglucose and 5 mmol/L Dglucose, then the osteogenic differentiation indexes of PDLSCs such as the ALP activity, mRNA expressions of ALP, OCN, and protein expression of OPN were detected. To evaluate the effects of GSRd on oxidative stress of high glucoseinduced PDLSCs, the protein and mRNA expressions of SOD and CAT were detected.Results: High glucose treating decreased the ALP activity of PDLSCs(P<0.05), but 10 and 40 μmol/L GSRd could improve the ALP activity of PDLSCs significantly(P<0.05).Compared with the control group, GSRd upregulated the mRNA expressions of ALP, OCN, and protein expression of OPN(F=102.893, 56.061, 60.296, P<0.05). GSRd also upregulated the mRNA and protein expressions of CAT and SOD(F=48.393, 67.134, 29.837, 7.539,P<0.05).Conclusion: GSRd could improve the osteogenic differentiation of PDLSCs and reduce the oxidative stress of PDLSCs with high glucose treatment, and then improve the osteogenic differentiation of PDLSCs.

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更新日期/Last Update: 1900-01-01