[1]雷冬梅),郭瑞霞)#,等.GPR30下调对子宫内膜癌细胞及裸鼠移植瘤组织PI3K/Akt信号通路的影响[J].郑州大学学报(医学版),2015,(05):593-596.
 LEI Dongmei),GUO Ruixia),LIU Jiaxi),et al.Influence of downregulation of GPR30 on PI3K/Akt signaling pathway in endometrial carcinoma cells and tumor tissue of nude mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(05):593-596.
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GPR30下调对子宫内膜癌细胞及裸鼠移植瘤组织PI3K/Akt信号通路的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年05期
页码:
593-596
栏目:
论著
出版日期:
2015-09-20

文章信息/Info

Title:
Influence of downregulation of GPR30 on PI3K/Akt signaling pathway in endometrial carcinoma cells and tumor tissue of nude mice
作者:
雷冬梅1) 郭瑞霞2)#刘泇希1) 葛 新2) 乔玉环2)
1)郑州大学第三附属医院病理科 郑州4500522)郑州大学第一附属医院妇产科 郑州 450052
Author(s):
LEI Dongmei1) GUO Ruixia2)LIU Jiaxi1)GE Xin2)QIAO Yuhuan2)
1)Department of Pathology,the Third Affiliated Hospital,Zhengzhou University, Zhengzhou 450052 2)Department of Obstetrics and Gynecology,the First Affiliated Hospital,Zhengzhou University, Zhengzhou 450052
关键词:
子宫内膜肿瘤受体G蛋白偶联磷脂酰肌醇3激酶AktHEC1A细胞Ishikawa细胞裸鼠
Keywords:
sendometrial neoplasmreceptorG proteincoupledPI3KAktHEC1A cellIshikawa cellnude mouse
分类号:
R737.33
文献标志码:
A
摘要:
目的:探讨G蛋白偶联受体(GPR30)下调对子宫内膜癌细胞及裸鼠移植瘤组织PI3K/Akt信号通路的影响。方法:采用免疫细胞化学SP法观察子宫内膜癌细胞系HEC1A和Ishikawa细胞中GPR30、Akt和磷酸化Akt(pAkt)蛋白的定位。以pGFPVRS(对照)和 pGFPVRSGPR30(干扰)分别转染两种细胞,用蛋白印迹法检测两种细胞中GPR30、Akt及pAkt蛋白的表达水平。构建裸鼠移植瘤模型(分别接种上述4种转染细胞,共4组),用免疫组化SP法检测4组裸鼠移植瘤组织中pAkt蛋白的表达。结果:①HEC1A和Ishikawa细胞中,GPR30、Akt和pAkt蛋白阳性表达均呈棕黄色,定位于细胞质。②稳定转染pGFPVRSGPR30的两种细胞株中GPR30和pAkt的表达均下调(P<0.05)。③与接种转染pGFPVRS细胞的2组裸鼠相比,接种转染pGFPVRSGPR30细胞的2组裸鼠移植瘤组织中pAkt表达降低。结论:在子宫内膜癌细胞及裸鼠移植瘤组织中,GPR30下调可能抑制了PI3K/Akt通路的活化。
Abstract:
To investigate the influence of downregulation of G proteincoupled estrogen receptor 30(GPR30) on activation of PI3K/Akt signaling pathway in endometrial carcinoma cells and tumor tissue of nude mice. Methods: The location of GPR30, Akt and pAkt protein in HEC1A and Ishikawa cells was detected by immunocytochemical SP method. The expression of GPR30 in HEC1A and Ishikawa cells was downregulated by transfection with pGFPVRSGPR30, a GPR30 antisense expression vector, and the cells transfected with pGFPVRS were the control; the levels of GPR30,Akt and pAkt were detected by Western blot. The nude mice were allocated into 4 groups and inoculated the above transfected cells, and immunohistochemistry was performed to observe the changes of the expression level of pAkt in the xenograft tissue.Results: Immunocytochemical SP method showed that GPR30, Akt and pAkt was stained as brown and yellow in cell cytoplasm of HEC1A and Ishikawa cells. Western blot analysis showed that the expressions of GPR30 and pAkt were decreased in tumor cells transfected by pGFPVRSGPR30 compared with the control (P<0.05). The expression of pAkt was decreased in the mice inoculated the tumor cells transfected by pGFPVRSGPR30.Conclusion: Downregulation of GPR30 inhibit the activation of PI3K/Akt signaling pathway in Ishikawa and HEC1A cells and tumor tissue of nude mice.

参考文献/References:

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更新日期/Last Update: 1900-01-01