[1]谢 毅△,张 超.精氨酸联合加味大柴胡汤对急性胆管炎大鼠肝脏功能的保护作用[J].郑州大学学报(医学版),2015,(05):706-709.
 XIE Yi,ZHANG Chao.Protective effects of arginine and modified major decoction of bupleurum on liver of rats with acute cholangitis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(05):706-709.
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精氨酸联合加味大柴胡汤对急性胆管炎大鼠肝脏功能的保护作用()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年05期
页码:
706-709
栏目:
应用研究
出版日期:
2015-09-20

文章信息/Info

Title:
Protective effects of arginine and modified major decoction of bupleurum on liver of rats with acute cholangitis
作者:
谢 毅张 超
河南省人民医院腹腔镜治疗研究中心 郑州 450003
Author(s):
XIE YiZHANG Chao
Department of Endoscopic Treatment and Research Center,Henan Provincial People's Hospital, Zhengzhou 450003
关键词:
急性胆管炎 加味大柴胡汤 精氨酸 TLR4 大鼠
Keywords:
acute cholangitis modified major decoction of bupleurum arginine TLR4 rat
分类号:
R656
文献标志码:
A
摘要:
目的:探讨加味大柴胡汤联合精氨酸对急性胆管炎大鼠肝脏功能的保护作用。方法:90只大鼠分为对照组、西药组、中药组、联合组和假手术组,每组18只。前4组建立胆道急性感染模型后,对照组给予生理盐水灌胃、西药组给予300 mg/kg精氨酸、中药组给予加味大柴胡汤(2 mL/次,2 次/d)灌胃、联合组同上给予精氨酸和加味大柴胡汤; 假手术组给予生理盐水灌胃。分别于处理后24、48及72 h取6只大鼠,取血测血浆内毒素与血清TB; 同时取肝组织,分别采用RT-PCR及免疫组化法检测TLR4 mRNA及蛋白的表达。结果:与假手术组相比,处理后24、48及72 h,对照组、西药组、中药组和联合组造模后血清TB水平、血浆内毒素水平及肝组织TLR4 mRNA表达水平升高,TLR4蛋白的表达增强; 与对照组相比,西药组、中药组及联合组以上指标均降低,其中联合组以上各指标最低(P<0.05)。结论:精氨酸联合加味大柴胡汤可能通过下调TLR4来降低内毒素水平从而减轻急性胆管炎大鼠的肝脏损害。
Abstract:
Aim: To explore the influence and mechanism of modified major decoction of bupleurum(MMDB)and arginine(Arg)on liver function of rats with acute cholangitis.Methods: A total of 90 rats were allocated into control group, western medicine group, medical herbs group, western medicine plus medical herbs group, and sham operation group(18 rats in each group). The first 4 groups were made acute cholangitis model. The rats in control group, western medicine group, medical herbs group, western medicine plus medical herbs group were given normal saline, Arg at 300 mg/kg,MMDB(2 mL per time and twice per day), and Arg plus MMDB,respectively. At 24,48 and 72 h after the above treatment, 6 rats were taken and the blood samples were prepared for determining plasma endotoxin and serum TB; the liver samples were prepared for detecting the expressions of TLR mRNA and protein by RT-PCR and immunohistochemical method,respectively.Results: Compared with sham operation group, serum TB and plasma endotoxin and the expressions of TLR mRNA and protein in control group, western medicine group, medical herbs group, western medicine plus medical herbs group were increased; compared with control group, the above indexes in western medicine group, medical herbs group, and western medicine plus medical herbs group were decreased, among which, those in western medicine plus medical herbs group were the lowest(P<0.05).Conclusion: MMDB plus Arg could reduce the level of plasma endotoxin by down-regulating the level of TLR4, thus alleviate the liver injury in rats with acute cholangitis.

参考文献/References:

[1] 郭丽娜,张文青,安阳,等.不同剂量精氨酸强化的肠内营养对创伤危重患者的支持作用[J].中华临床医师杂志:电子版,2015,9(7):1093
[2] 林木生,王三明,包仕廷,等.加味大柴胡汤加用L-精氨酸对阻黄大鼠血浆内毒素的影响[J].中华实验外科杂志,2004,21(10):1202
[3] 段吉明,李文星,张毅,等.内毒素对大鼠淋巴细胞表达TLR4及NF-κB 与分泌 IL-6 的影响[J].中华临床医师杂志:电子版,2014,8(18):3319
[4] 黄继汉,黄晓晖,陈志扬,等.药理试验中动物间和动物与人体间的等效剂量换算[J].中国临床药理学与治疗学,2004,9(9):1069
[5] Wang H,Ma S.The cytokine storm and factors determining the sequence and severity of organ dysfunction in multiple organ dysfunction syndrome[J].Am J Emerg Med,2008,26(6):711
[6] Szabo C,Dolganiuc A,Mandrekar P.Pattern recognition receptors:a contemporary view on liver diseases[J].Hepatology,2006,44(2):287
[7] Tsujimoto H,Ono S,Efron PA,et al.Role of Toll-like receptors in the development of sepsis[J].Shock,2008,29(3):315
[8] Hisaeda K,Inokuchi A,Nakamura T,et al.Interleukin-1beta represses MRP2 gene expression through inactivation of interferon regulatory factor 3 in HepG2 cells[J].Hepatology,2004,39(6):1574
[9] 杨错,邢立国,刘玉兰.柴胡对大鼠实验性肝纤维化的预防作用[J].实用药物与临床,2005,8(5):12
[10]李立萍,张建新,董淑婷,等.L-硝基精氨酸对大鼠LPS性肺损伤的实验治疗研究[J].中国病理生理杂志,2004,20(5):769

更新日期/Last Update: 1900-01-01