[1]牛朝霞,李宁宁,陈洁,等.RNA干扰沉默survivin基因对Eca109细胞增殖与凋亡的影响[J].郑州大学学报(医学版),2015,(06):737-739.
 NIU Zhaoxia,LI Ningning,CHEN Jie,et al.Effects of silencing survivin gene by RNA interference on proliferation and apoptosis of Eca109 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(06):737-739.
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RNA干扰沉默survivin基因对Eca109细胞增殖与凋亡的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年06期
页码:
737-739
栏目:
食管癌研究
出版日期:
2015-12-20

文章信息/Info

Title:
Effects of silencing survivin gene by RNA interference on proliferation and apoptosis of Eca109 cells
作者:
牛朝霞李宁宁陈洁李宜培彭蕤蕤秦紫芳
河南医学高等专科学校病理生理学教研室 郑州 451191
Author(s):
NIU ZhaoxiaLI NingningCHEN JieLI YipeiPENG RuiruiQIN Zifang
Department of Pathophysiology,Henan Medical College,Zhengzhou 451191
关键词:
RNA干扰survivin细胞增殖细胞凋亡Eca109细胞
Keywords:
RNA interferencesurvivincell proliferationcell apoptosisEca109 cell
分类号:
R735.1
摘要:
目的:通过RNA干扰抑制食管癌细胞Eca109 survivin基因的表达,观察survivin基因沉默对Eca109细胞增殖与凋亡的影响。方法:构建靶向survivin基因的siRNA表达载体并借助脂质体稳定转染Eca109细胞(干扰组),同时设转染无关干扰质粒的Eca109细胞为阴性对照,未转染细胞为空白对照。采用Western blot检测survivin基因沉默效果,MTT、平板克隆形成实验及流式细胞术分析干扰前后Eca109细胞增殖与凋亡的变化。结果:与两对照组相比,干扰组Survivin蛋白的表达降低(F=79.397,P<0.001);MTT检测结果显示,干扰组抑制细胞增殖效果优于两对照组,而平板克隆形成实验显示干扰组克隆形成率低于两对照组(F=162.026,P<0.001);流式细胞术分析显示,干扰组凋亡指数高于两对照组(F=2 284.205,P<0.001)。结论:RNA干扰可特异性沉默survivin基因的表达,抑制Eca109细胞的增殖,诱导细胞凋亡。
Abstract:
To inhibit endogenous survivin expression by RNA interference technology and investigate the effects on proliferation and apoptosis of Eca109 cells.Methods: An siRNA expressing vector targeting survivin was constructed and transfected into Eca109 cells via LipofectamineTM2000 to establish stable transfection cell line.The Eca109 cells transfected nonsense sequence and those untransfected were used as control.The expression level of Survivin protein was detected by Western blot to observe the interference effect; cell proliferation of Eca109 cells was determined by MTT assay and colony formation assay; cell apoptosis of Eca109 cells was measured by flow cytometry analysis.Results: Compared with the 2 control groups, the expression level of Survivin protein in the interference group was significantly reduced(F=79.397,P<0.001); cell proliferation in the interference group was distinctly inhibited(F=162.026,P<0.001); the apoptosis index in the interference group was significantly increased(F=2 284.205,P<0.001). Conclusion: Knockdown of endogenous survivin via specific RNA interference can effectively inhibit cell proliferation and significantly induce cell apoptosis of Eca109 cells.

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更新日期/Last Update: 1900-01-01