[1]晁宏图,李晓凤,寇馨歆,等.LBH589对OVCAR3细胞增殖、侵袭和血管内皮生长因子表达的影响[J].郑州大学学报(医学版),2015,(06):769-772.
 CHAO Hongtu,LI Xiaofeng,KOU Xinxin,et al.Effects of LBH589 on proliferation,invasion,and VEGF expression of OVCAR3 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(06):769-772.
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LBH589对OVCAR3细胞增殖、侵袭和血管内皮生长因子表达的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年06期
页码:
769-772
栏目:
论著
出版日期:
2015-12-20

文章信息/Info

Title:
Effects of LBH589 on proliferation,invasion,and VEGF expression of OVCAR3 cells
作者:
晁宏图李晓凤寇馨歆李雷杨晓霞王莉英王莉#
河南省肿瘤医院(郑州大学附属肿瘤医院妇瘤科 郑州 450003
Author(s):
CHAO HongtuLI XiaofengKOU XinxinLI LeiYANG XiaoxiaWANG LiyingWANG Li
Department of Gynecologic Oncology,Henan Cancer Hospital(the Affiliated Cancer Hospital,Zhengzhou University,Zhengzhou 450003
关键词:
组蛋白去乙酰化酶抑制剂OVCAR3细胞增殖侵袭VEGFPI3K/Akt通路
Keywords:
histone deacetylase inhibitorOVCAR3 cellproliferationinvasionVEGFPI3K/Akt pathway
分类号:
R737.31
摘要:
目的:观察LBH589对上皮性卵巢癌OVCAR3细胞增殖、侵袭和VEGF表达的影响。方法:采用MTT法和Transwell实验分别检测1、5、10 μmol/L的LBH589对OVCAR3细胞活性和侵袭细胞数的影响,采用Western blot法检测Ki67、MMP2、VEGF、pPI3K、pAkt蛋白的表达水平。采用MTT和Western blot法分别检测PI3K抑制剂LY294002对细胞活性以及MMP2、VEGF表达水平的影响。结果:LBH589降低OVCAR3细胞活性(F=5.324,P<0.001),减少穿过Transwell基质膜的细胞数量(F=84.325,P<0.001),Ki67、MMP2、VEGF、pPI3K、pAkt蛋白的表达水平亦降低(F=9.191、7.623、6.340、6.221、4.690,P均<0.001)。与对照组相比,LY294002抑制MMP2和VEGF蛋白的表达,并降低OVCAR3细胞活性(P均<0.05)。结论:LBH589可能通过调控PI3K/Akt信号通路下调MMP2和VEGF表达水平,继而抑制上皮性卵巢癌OVCAR3细胞增殖、侵袭和血管生成。
Abstract:
Aim: To observe the effects of LBH589 on the proliferation, invasion and VEGF expression of OVCAR3 cells.Methods: The effects of 1, 5, and 10 μmol/L LBH589 on the relative viability and invasion of OVCAR3 cells were determined using MTT and Transwell assay, respectively. The effects of LBH589 on the expression levels of Ki67, MMP2, VEGF, pPI3K, and pAkt were measured using Western blot analysis. Finally, the effects of PI3K inhibitor LY294002 on the relative viability and the expression levels of MMP2 and VEGF were detected using MTT assay and Western blot analysis, respectively.Results: LBH589 lowered the relative viability of OVCAR3 cells(F=5.324,P<0.001), reduced the number of transferred cells(F=84.325,P<0.001). Also, the expression levels of Ki67, MMP2, VEGF, pPI3K, and pAkt decreased(F=9.191,7.623,6.340,6.221,4.690,P<0.001). Compared with the control group, LY294002 downregulated the expressions of MMP2 and VEGF, and lowered the relative viability of OVCAR3 cells(P<0.05).Conclusion: LBH589 downregulates the expressions of MMP2 and VEGF by mediating the PI3K/Akt signaling pathway, resulting in the inhibition of proliferation, invasion and angiogenesis of OVCAR3 cells.

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更新日期/Last Update: 1900-01-01