[1]王勇,赵伟,马骥#.Notch1和HES1在骨肉瘤组织中的表达及其对U2OS细胞侵袭能力的影响[J].郑州大学学报(医学版),2015,(06):777-780.
 WANG Yong,ZHAO Wei,MA Ji.Expressions of Notch1 and HES1 in osteosarcoma tissue and their effects on invasion ability of osteosarcoma cell U2OS[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(06):777-780.
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Notch1和HES1在骨肉瘤组织中的表达及其对U2OS细胞侵袭能力的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年06期
页码:
777-780
栏目:
论著
出版日期:
2015-12-20

文章信息/Info

Title:
Expressions of Notch1 and HES1 in osteosarcoma tissue and their effects on invasion ability of osteosarcoma cell U2OS
作者:
王勇赵伟马骥#
沈阳医学院附属中心医院骨四科 沈阳 110024
Author(s):
WANG YongZHAO WeiMA Ji
The 4th Department of Orthopaedics,the Affiliated Central Hospital,Shenyang Medical College,Shenyang 110024
关键词:
Notch1HES1骨肉瘤侵袭
Keywords:
Notch1HES1osteosarcomainvasion
分类号:
R738.7
摘要:
目的:研究Notch1和HES1基因在骨肉瘤组织及骨肉瘤细胞系U2OS中的表达及其对U2OS侵袭能力的影响。方法:组织学水平研究中,配对选取23例骨肉瘤及临近瘤旁正常骨骼肌组织标本;细胞学水平研究中,选取骨肉瘤细胞系U2OS及人正常成骨细胞系hFOB1.19;采用免疫组化法检测组织学水平Notch1及HES1蛋白的表达,采用Western blot及Realtime PCR法检测组织学及细胞学水平Notch1、HES1蛋白及mRNA的表达。用Notch1siRNA沉默U2OS细胞中Notch1基因后,应用Western blot及Realtime PCR法检测U2OS细胞中Notch1及HES1的表达,Transwell法检测U2OS侵袭能力的变化。结果:相比于正常组织,骨肉瘤组织中Notch1、HES1 mRNA和蛋白呈高表达(t配对=22.567,14.830和8.439,7.100,P<0.001)。U2OS细胞中Notch1、HES1 mRNA和蛋白表达强于hFOB1.19细胞(t=11.053,20.482和28.845,34.681,P<0.001)。沉默Notch1基因后,U2OS细胞Notch1、HES1 mRNA和蛋白表达下降(t=6.294,9.209和9.151,13.953,P<0.05),侵袭能力下降(t=71.387,P<0.001)。 结论:Notch1和HES1在骨肉瘤组织及骨肉瘤细胞系中呈高表达,沉默Notch1基因可降低U2OS细胞侵袭能力。
Abstract:
Aim: To investigate the expressions of Notch1 and HES1 gene in osteosarcoma tissue and their effects on invasion ability of osteosarcoma cell U2OS.Methods: Twentythree matched tissue specimens of osteosarcoma and matched paratumor normal tissue, and osteosarcoma cell line U2OS and human osteoblast cell line hFOB1.19 were chosen. The expressions of Notch1 and HES1 mRNA and protein in tissue specimens were detected by immunohistochemistry,Realtime PCR and Western blot. The expressions of Notch1 and HES1 mRNA and protein in hFOB1.19,U2OS, and U2OS transfected with Notch1siRNA were detected by Realtime PCR and Western blot, and the cell invasion ability was detected by Transwell chambers.Results: Compared with paratumor normal tissue, the Notch1 and HES1 mRNA and protein expressions in osteosarcoma tissue were higher(tmatch=22.567,14.830 and 8.439,7.100,P<0.001), and those in U2OS were higher than in hFOB1.19(t=11.053,20.482 and 28.845,34.681,P<0.001). Compared with U2OS, the Notch1 and HES1 mRNA and protein expressions in U2OS transfected with Notch1siRNA were lower(t=6.294,9.209 and 9.151,13.953,P<0.05), and the invasion ability decreased(t=71.387,P<0.001). Conclusion: Notch1 and HES1 gene show high expression in both tissue and cellular level in osteosarcoma; Notch1 silence can reduce the invasion ability of U2OS.

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更新日期/Last Update: 1900-01-01