[1]王碧君,郭艺红#,张慧红,等.PPARγ上调对PCOS患者卵巢颗粒细胞P450arom mRNA的表达及T转化效应的影响[J].郑州大学学报(医学版),2015,(06):790-793.
 WANG Bijun,GUO Yihong,ZHANG Huihong,et al.Effects of upregulation of PPARγ on expression of P450arom mRNA and T transforming effects in ovarian granulosa cells from patients with PCOS[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(06):790-793.
点击复制

PPARγ上调对PCOS患者卵巢颗粒细胞P450arom mRNA的表达及T转化效应的影响()
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年06期
页码:
790-793
栏目:
论著
出版日期:
2015-12-20

文章信息/Info

Title:
Effects of upregulation of PPARγ on expression of P450arom mRNA and T transforming effects in ovarian granulosa cells from patients with PCOS
作者:
王碧君郭艺红#张慧红李婧杨新红
郑州大学第一附属医院生殖医学中心 郑州 450052
Author(s):
WANG Bijun GUO Yihong ZHANG Huihong LI Jing YANG Xinhong
Reproductive Medicine Center, the First Affiliated Hospital, Zhengzhou University,Zhengzhou 450052
关键词:
多囊卵巢综合征卵巢颗粒细胞过氧化物酶体增殖物激活受体γ细胞色素P450芳香化酶T转化效应
Keywords:
polycystic ovarian syndromeovarian granulosa cellperoxisome proliferator activated receptor γcytochrome P450 aromataseT transforming effect
分类号:
R711.6
摘要:
目的:探讨PPARγ上调对PCOS患者卵巢颗粒细胞P450arom mRNA表达的调控作用及对T转化效应的影响。方法:提取10例雄激素正常的PCOS 患者(NAPCOS组)、10例伴高雄激素血症的PCOS患者(HAPCOS组)及10例仅因输卵管因素不孕患者(对照组)的卵巢颗粒细胞,分别给予0、1、10 nmol/L的PPARγ激动剂罗格列酮培养48 h后,采用RTPCR测定PPARγ、P450arom mRNA的表达,采用 ELISA法测定培养液中E2值,计算T转化率。结果:PPARγ上调后,3组患者P450arom mRNA的表达均降低,T转化率下降,尤其是HAPCOS组(P<0.05)。PPARγ上调(10 nmol/L罗格列酮处理)后,HAPCOS组卵巢颗粒细胞中PPARγ mRNA的表达水平和T转化率负相关,P450arom mRNA的表达水平和T转化率正相关(r=-0.633、0.829,P<0.05)。结论:PPARγ可能通过抑制P450arom参与PCOS高雄激素血症的发生。
Abstract:
Aim: To explore the effects of upregulation of PPARγ on expression of P450arom mRNA and T transforming effects in the ovarian granulosa cells from patients with polycystic ovarian syndrome

参考文献/References:


[1]易思思,陈薪,刘玉东,等.多囊卵巢综合征患者反复种植失败后成功妊娠3例及其文献复习[J].南方医科大学学报,2014,34(9):1329
[2]王丽,吕淑兰,曹缵孙.青春期与育龄期多囊卵巢综合征患者的临床对照研究[J].西安交通大学学报:医学版,2010,31(6):745
[3]Lebbe M,Woodruff TK.Involvement of androgens in ovarian health and disease[J].Mol Hum Reprod,2013,19(12):828
[4]García Barrado MJ,Blanco EJ,Carretero Hernández M,et al.Local transformations of androgens into estradiol by aromatase P450 is involved in the regulation of prolactin and the proliferation of pituitary prolactinpositive cells[J].PLoS One,2014,9(6):e101403
[5]王芳芳,曲凡,黄荷凤,等.多囊卵巢综合征患者卵巢局部高雄与颗粒细胞PPARγ的表达[C].中国杭州生殖健康学术论坛暨浙江省计划生育学与生殖医学分会学术年会,2009.
[6]孙莹璞,苏迎春,郭艺红,等.体外受精胚胎移植技术在常规超排卵304个治疗周期中的应用[J].郑州大学学报:医学版,2002,37(1):1
[7]胡卫红,陈琳,同军,等.PPARγ mRNA在卵巢颗粒细胞的表达调节及与多囊卵巢综合征的相关性[J].北京大学学报:医学版,2013,45(6):859
[8]Hao CF,Zhang N,Qu Q,et al.Evaluation of the association between the CYP19 Tetranucleotide(TTTA)n polymorphism and polycystic ovarian syndrome(PCOS) in Han Chinese women[J].Neuro Endocrinol Lett,2010,31(3):370
[9]Barroso G,Menocal G,Felix H,et al.Comparison of the efficacy of the aromatase inhibitor letrozole and clomiphene citrate as adjuvants to recombinant folliclestimulating hormone in controlled ovarian hyperstimulation:a prospective,randomized,blinded clinical trial[J].Fertil Steril,2006,86(5):1428
[10]Maliqueo M,Sun M,Johansson J,et al.Continuous administration of a P450 aromatase inhibitor induces polycystic ovary syndrome with a metabolic and endocrine phenotype in female rats at adult age[J].Endocrinology,2013,154(1):434
[11]Faut M,Elia EM,Parborell F,et al.Peroxisome proliferatoractivated receptor gamma and early folliculogenesis during an acute hyperandrogenism condition[J].Fertil Steril,2011,95(1):333
[12]Lazaros L,Xita N,Hatzi E,et al.CYP19 gene variants affect the assisted reproduction outcome of women with polycystic ovary syndrome[J].Gynecol Endocrinol,2013,29(5):478
[13]SanMillán JL,EscobarMorreale HF.The role of genetic variation in peroxisome proliferatoractivated receptors in the polycystic ovary syndrome(PCOS):an original casecontrol study followed by systematic review and metaanalysis of existing evidence[J].Clin Endocrinol(Oxf),2010,72(3):383
[14]Koika V,Marioli DJ,Saltamavros AD,et al.Association of the Pro12Ala polymorphism in peroxisome proliferatoractivated receptor gamma2 with decreased basic metabolic rate in women with polycystic ovary syndrome[J].Eur J Endocrinol,2009,161(2):317

相似文献/References:

[1]陈志敏,黄华,史惠蓉#.二甲双胍与罗格列酮治疗多囊卵巢综合征临床效果评价[J].郑州大学学报(医学版),2009,(01):164.
 CHEN Zhimin,HUANG Hua,SHI Huirong.Effects of metformin and roslglitazoneon in the treatment of polycystic ovarian syndrome[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(06):164.
[2]孙莹璞△,王芳,孔慧娟,等.多囊卵巢综合征来源人胚胎干细胞系的建立及成脂分化[J].郑州大学学报(医学版),2010,(04):549.
[3]谷保霞,张翠莲,李杭生.胰岛素增敏剂联合来曲唑治疗多囊卵巢综合征84例[J].郑州大学学报(医学版),2010,(04):675.
 [J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(06):675.
[4]管一春)△,杨雪峰),王兴玲),等. 多囊卵巢综合征患者子宫内膜组织中同源框基因A10蛋白的表达[J].郑州大学学报(医学版),2011,(03):422.
 GUAN Yichun,YANG Xuefeng,WANG Xingling,et al. Expression of homeobox gene A10 in endometria of polycystic ovary syndrome patients[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(06):422.
[5]翟军),孙莹璞)#. 二甲双胍对PCOS患者血清TNFα水平及内分泌代谢的影响[J].郑州大学学报(医学版),2011,(02):235.
 ZHAI Jun),SUN Yingpu). Effect of metformin on serum TNFα levels,endocrine and metabolic festures of PCOS patients[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(06):235.
[6]贾莉婷△,刘艳丽,马葆靖,等.两种多囊卵巢综合征大鼠模型造模方法的比较*[J].郑州大学学报(医学版),2011,(04):538.
 JIA Liting,LIU Yanli,MA Baojing,et al.Comparison of two kinds of polycystic ovary syndrome rat models[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(06):538.
[7]李根霞△,李巍巍.多囊卵巢综合征患者胰岛素抵抗和氧化应激的关系[J].郑州大学学报(医学版),2011,(05):759.
 LI Genxia,LI Weiwei.Relationship between insulin resistance and oxidative stress in patients with PCOS[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(06):759.
[8]梁琳琳△,李杭生,何巧花,等.IVF周期中不同亚型多囊卵巢综合征不孕患者低剂量递增促排卵效果*[J].郑州大学学报(医学版),2012,(04):530.
 LIANG Linlin,LI Hangsheng,HE Qiaohua,et al.Comparison of clinical results of lowdose increasing ovarian stimulation in IVF patients with different subtype of polycystic ovary syndrome[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(06):530.
[9]马丽影,项云改,李艳,等.体外受精胚胎移植治疗多囊卵巢综合征不孕患者临床效果[J].郑州大学学报(医学版),2012,(05):701.
 MA Liying,XIANG Yungai,LI Yan,et al.Clinical analysis of in vitro fertilization and embryo transfer in infertility women with polycystic ovary syndrome[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(06):701.
[10]杨菲),邓琳琳).氟他胺联合二甲双胍治疗难治性多囊卵巢综合征疗效观察[J].郑州大学学报(医学版),2013,(04):562.

更新日期/Last Update: 1900-01-01