[1]葛丽丽,朴军颜,杨小昂#,等.沉默FATE/BJHCC2基因的表达对肝癌细胞迁移及侵袭的影响[J].郑州大学学报(医学版),2015,(06):805-808.
 GE Lili,PIAO Junyan,YANG Xiaoang,et al.Effects of silencing FATE/BJHCC2 gene on migration and invasion of hepatocellular carcinoma cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(06):805-808.
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沉默FATE/BJHCC2基因的表达对肝癌细胞迁移及侵袭的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年06期
页码:
805-808
栏目:
论著
出版日期:
2015-12-20

文章信息/Info

Title:
Effects of silencing FATE/BJHCC2 gene on migration and invasion of hepatocellular carcinoma cells
作者:
葛丽丽12朴军颜1杨小昂1# 尹艳慧3张毓3
1郑州大学医药科学研究院 郑州 4500522郑州市儿童医院检验科 郑州 4500533北京大学免疫学系T细胞实验室 北京 100083
Author(s):
GE Lili12PIAO Junyan1YANG Xiaoang1YIN Yanhui3ZHANG Yu3
1Academy of Medical and Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 4500522Clinical Laboratory,Zhengzhou Children′s Hospital,Zhengzhou 4500533Department of Immunology,Health Sciences Center,Peking University,Beijing 100083
关键词:
FATE/BJHCC2RNA干扰肝癌转移
Keywords:
FATE/BJHCC2RNA interferenceliver cancermetastasis
分类号:
R735.7
摘要:
目的:研究FATE/BJHCC2基因表达与肝癌细胞迁移及侵袭的关系。方法:体外合成FATE/BJHCC2基因序列特异性小干扰RNA(siRNA),转染肝癌细胞系克隆株5B4,并设非特异性siRNA转染和空白对照组。采用RTPCR及Western blot方法检测各组细胞中 FATE/BJHCC2 mRNA和蛋白的表达;通过Transwell小室模型检测各组细胞体外迁移及侵袭的能力;激光共聚焦显微镜下观察5B4及空质粒转染的对照细胞(Mock)的骨架结构。结果:各组细胞中FATE/BJHCC2 mRNA和蛋白表达差异有统计学意义(F=15.321,P=0.020),特异性siRNA转染组细胞低于其他2组(P<0.05)。抑制FATE/BJHCC2基因表达后,细胞体外迁移及侵袭的能力降低(F=6.171和10.109,P<0.05)。5B4细胞形态拉长,蝌蚪状,呈现迁移、游走的状态;微丝丰富,形态规则,平行贯穿于细胞全长,细胞膜周围有较多丝状伪足伸出;微管减少,分布不规则。结论:FATE/BJHCC2基因表达促进肝癌细胞的迁移及侵袭。
Abstract:
Aim: To study the effects of FATE/BJHCC2 gene expression on the migration and invasion of hepatocellular carcinoma(HCC) cells.Methods: The HCC cell clone line 5B4 cells was transfected with FATE/BJHCC2 gene sequencespecific small interference RNA(siRNA) synthesised in vitro.The expressions of FATE/BJHCC2 mRNA and protein were detected by RTPCR and Western blot,respectively.The migration and invasion ability of cells were examined by Transwell assay. The cytoskeleton of 5B4 cells transfected FATE/BJHCC2 and empty vector control(Mock) cells was observed by laser scanning confocal microscope.The 5B4 cells not treated were used as blank control.Results: Both of mRNA and protein expressions declined in the group of cells transfected with FATE/BJHCC2 sequencespecific siRNA(F=15.321,P=0.020). The migration and invasion ability of 5B4 cells obviously reduced after FATE/BJHCC2 gene was silenced (F=6.171 and 10.109,P<0.05). Compared with that of the mock cells, the morphological and cytoskeleton structure of 5B4 cells transfected with FATE/BJHCC2 changed obviously. Conclusion: FATE/BJHCC2 gene expression promotes migration and invasion of hepatocellular carcinoma cells.

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