[1]赵华,王兴玲,李丽,等.人工干预对反复种植失败患者着床窗口期子宫内膜组织中LPAR3和HOXA11表达的影响[J].郑州大学学报(医学版),2015,(06):814-817.
 ZHAO Hua,WANG Xingling,LI Li,et al.Influence of manual intervention on LPAR3 and HOXA11 expressions at the endometrial implantation period in RIF patients[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(06):814-817.
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人工干预对反复种植失败患者着床窗口期子宫内膜组织中LPAR3和HOXA11表达的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年06期
页码:
814-817
栏目:
应用研究
出版日期:
2015-12-20

文章信息/Info

Title:
Influence of manual intervention on LPAR3 and HOXA11 expressions at the endometrial implantation period in RIF patients
作者:
赵华1王兴玲2李丽2 李豫峰1#
1华中科技大学附属同济医院生殖中心 武汉 4300002郑州大学第三附属医院生殖中心 郑州 450052
Author(s):
ZHAO Hua1WANG Xingling2LI Li2LI Yufeng1
1The Reproduction Center, Tongji Hospital,Huazhong University of Science and Technology, Wuhan 4300002The Reproduction Center, the Third Affiliated Hospital, Zhengzhou University,Zhengzhou 450052
关键词:
子宫内膜容受性子宫内膜搔刮术同源框基因11溶血磷脂酸受体3调经促孕丸反复种植失败
Keywords:
uterine endometrial receptivityuterine endometrial scratchingHOXA11LPAR3progestational pillrepeated implantation failure
分类号:
R711
摘要:
目的:探讨对反复种植失败(RIF)患者进行人工干预后,着床窗口期子宫内膜组织中溶血磷脂酸受体3(LPAR3)和同源框基因11(HOXA11)表达变化对临床结局的影响。方法:将92例因输卵管因素不孕的RIF患者分为搔刮组(子宫内膜搔刮,n=31)、中成药组(调经促孕丸,n=31)、未干预组(n=30)。采用RTPCR和Western blot方法检测3组患者窗口期子宫内膜组织中LPAR3和HOXA11 mRNA和蛋白表达的情况,并观察3组患者临床妊娠率的差异。结果:搔刮组、中成药组患者窗口期子宫内膜组织中LPAR3和HOXA11 mRNA及蛋白表达量高于未干预组,差异有统计学意义(F=57.463和60.771,P<0.001;F=48.816和40.313,P<0.001),而前两组比较差异无统计学意义(P>0.05)。搔刮组、中成药组复苏周期临床妊娠率分别是48.4%和32.3%,均高于未干预组(16.7%),差异有统计学意义(χ2=6.908,P=0.032),但前两组比较差异无统计学意义(P>0.05)。结论:子宫内膜搔刮术和口服调经促孕丸2种干预措施均可以改善子宫内膜容受性,提高RIF患者的临床妊娠率。
Abstract:
Aim: To investigate the expressions and significance of LPAR3 and HOXA11 in the mRNA and protein level at the endometrial implantation phase after the manual intervention in the patients with repeated implantation failure(RIF).Methods: According to the RIF patients′ willingness of receiving manual intervention and the different intervention methods, the 92 RIF patients with fallopian tuberelated infertility were allocated into scratching group (n=31), Chinese patent medicine group (n=31) and nonintervention group (n=30).RTPCR and Western blot were adopted to detect the expressions of LPAR3 and HOXA11 mRNA and protein at the endometrial implantation phase after the ovulation of the RIF patients. Results: The results of RTPCR and Western blot showed that the expressions of LPAR3 and HOXA11 mRNA and protein in the scratching group and Chinese patent medicine group at the implantation window phase noticeably higher than those in the nonintervention group(F=57.463 and 60.771,P<0.001;F=48.816 and 40.313,P<0.001),however, there was no statistical difference between the former two groups. The clinical pregnancy rate(48.4%) in the scratching group and Chinese patent medicine group(32.3%) was higher than that in the nonintervention group(16.7%)(χ2=6.908, P<0.032),however, the comparison of the former 2 groups revealed no statistical difference (P>0.05).Conclusion: Two intervention measures, including the endometrial scratching and taking progestational pills, could improve the endometrial receptivity and enhance the clinical pregnancy rate of the RIF patients.

参考文献/References:


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更新日期/Last Update: 1900-01-01