[1]田甜,孙雅菲,于淼,等.脑动静脉畸形病变血管组织学变化和血管内皮生长因子的表达[J].郑州大学学报(医学版),2015,(06):831-833.
 TIAN Tian,SUN Yafei,YU Miao,et al.Histological changes and expression of VEGF in cerebral arteriovenous malformation vascular tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(06):831-833.
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脑动静脉畸形病变血管组织学变化和血管内皮生长因子的表达()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年06期
页码:
831-833
栏目:
应用研究
出版日期:
2015-12-20

文章信息/Info

Title:
Histological changes and expression of VEGF in cerebral arteriovenous malformation vascular tissue
作者:
田甜1孙雅菲2于淼1张继伟1呼铁民1王维兴1王广1#
1承德医学院附属医院神经外科 承德 0670002河北医科大学第二医院神经内科 石家庄 050000
Author(s):
TIAN Tian1SUN Yafei2YU Miao1ZHANG Jiwei1HU Tiemin1WANG Weixing1WANG Guang1
1Department of Neurosurgery,the Affiliated Hospital,Chengde Medical College,Chengde 067000 2Department of Internal Medicine, the Second Hospital,Hebei Medical University,Shijiazhuang 050000
关键词:
脑动静脉畸形组织学变化血管内皮生长因子
Keywords:
cerebral arteriovenous malformationhistological changevascular endothelial growth factor
分类号:
R743.4
摘要:
目的:探索脑动静脉畸形(CAVM)与脑血管内皮生长因子(VEGF)之间的关系。方法:对30例CAVM病变血管及病变周围血管组织标本行HE染色,观察血管的组织学改变。采用免疫组化法和Western blot法检测血管中VEGF蛋白的表达,采用荧光定量PCR法检测血管中VEGF mRNA的表达。以32例正常脑组织血管标本作对照。结果:CAVM病变血管和病变周围血管较正常脑血管发生明显改变。荧光定量PCR和Western blot检测结果显示,CAVM病变血管和病变周围血管组织中VEGF mRNA和蛋白表达水平均高于正常脑血管(P均<0.05),CAVM病变血管中VEGF mRNA和蛋白表达水平高于病变周围血管(t配对=19.095,3.216,P<0.05)。结论:VEGF可能对CAVM的发生和发展起着重要作用。
Abstract:
Aim: To explore the pathological characteristics of cerebral arteriovenous malformation(CAVM) and the relationship between CAVM and vascular endothelial growth factor(VEGF). Methods: A total of 30 CAVM and the peripheral vascular tissue were collected. Histological changes in vascular tissues were observed using HE staining.The expression of VEGF proteins were detected by immunohistochemistry and Western Blot. The expression of VEGF mRNA were detected by Realtime PCR.32 normal brain vascular tissue were the control. Results: The histological characteristics of CAVM and peripheral vascular tissue were obviously different from the normal brain vascular tissue. The results of Realtime PCR and Western blot showed that the expression of VEGF protein and mRNA in CAVM and peripheral vascular tissue were significantly higher than those in normal brain vascular tissue(P<0.05), and the expression of VEGF protein and mRNA in CAVM were higher than those in peripheral vascular tissue(tmatch=19.095,3.216,P<0.05). Conclusion: VEGF may play an important role in the occurrence and development of CAVM.

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更新日期/Last Update: 1900-01-01