[1]李玉山,陈河涛,杨夕阳,等.TEKT3在特发性弱精子症患者精子中的表达[J].郑州大学学报(医学版),2015,(06):843-846.
 LI Yushan,CHEN Hetao,YANG Xiyang,et al.Expression of TEKT3 in spermatozoa of idiopathic asthenozoospermia patients[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(06):843-846.
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TEKT3在特发性弱精子症患者精子中的表达()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2015年06期
页码:
843-846
栏目:
应用研究
出版日期:
2015-12-20

文章信息/Info

Title:
Expression of TEKT3 in spermatozoa of idiopathic asthenozoospermia patients
作者:
李玉山陈河涛杨夕阳王全先刘军杰王琳凯苏彦华孙琳
郑州大学第三附属医院人类精子库 郑州 450052
Author(s):
LI YushanCHEN HetaoYANG XiyangWANG QuanxianLIU JunjieWANG LinkaiSU YanhuaSUN Lin
Human Sperm Bank,the Third Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
TEKT3特发性弱精子症精子前向运动能力
Keywords:
TEKT3idiopathic asthenozoospermiasperm progressive motility
分类号:
R698.2
摘要:
目的:探讨特发性弱精子症患者精子中TEKT3表达的特点。方法:采用非连续密度梯度离心法分离、纯化正常对照组和弱精子症组精子标本各35份,分别采用RTPCR和Western blot检测精子中TEKT3 mRNA和蛋白的表达。结果:与正常对照组比较,弱精子症组TEKT3 mRNA和蛋白表达均显著降低(t=9.610、10.090,P<0.001),相关性分析显示弱精子症组精子TEKT3 mRNA和蛋白表达水平与前向运动精子率呈正相关(r=0.684,P=0.001;r=0.483,P=0.013)。结论:TEKT3表达降低可能在特发性弱精子症发病中具有重要作用。
Abstract:
Aim: To investigate the function of TEKT3 protein in the pathogenesis of patients with idiopathic asthenozoospermia.Methods: Semen samples were collected from 35 normal controls and 35 idiopathic asthenozoospermia patients and purified using Percoll discontinuous density gradients, then the expression of TEKT3 mRNA and TEKT3 protein were detected by RTPCR and Western blot. Results: Compared with normal controls, the expressions of TEKT3 mRNA and protein in spermatozoa of idiopathic asthenozoospermia group were obviously decreased(t=9.610,10.090,P<0.001). Correlation analysis revealed that the expressions of TEKT3 mRNA and protein were positively correlated with spermatozoa progressive motility(r=0.684,P=0.001;r=0.483,P=0.013). Conclusion: The reduced expression of TEKT3 may play an important role in the pathogenesis of idiopathic asthenozoospermia.

参考文献/References:


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相似文献/References:

[1]李玉山△,吉晓菲,王全先,等.特发性弱精子症患者精子中TCTE3的表达*[J].郑州大学学报(医学版),2014,(01):83.
 LI Yushan,JI Xiaofei,WANG Quanxian,et al.Expression of TCTE3 in spermatozoa of idiopathic asthenozoospermic patients[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(06):83.

更新日期/Last Update: 1900-01-01