[1]温胜兰,谢诗,王志伟,等.河南汉族男性原发性不育与SHBG基因启动子(TAAAA)n多态性的关联性[J].郑州大学学报(医学版),2016,(01):85-88.
 WEN Shenglan,XIE Shi,WANG Zhiwei,et al.Association of (TAAAA)n polymorphism in SHBG gene promoter with idiopathic male infertility in Henan Han population[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(01):85-88.
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河南汉族男性原发性不育与SHBG基因启动子(TAAAA)n多态性的关联性()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年01期
页码:
85-88
栏目:
应用研究
出版日期:
2016-01-20

文章信息/Info

Title:
Association of (TAAAA)n polymorphism in SHBG gene promoter with idiopathic male infertility in Henan Han population
作者:
温胜兰1谢诗1王志伟1何诺2李兴武3#陈辉4#
1)郑州大学临床医学系 郑州 4500522)郑州市中心医院检验科 郑州 4500073)郑州大学第一附属医院检验科 郑州 4500524)郑州大学基础医学院细胞生物学与医学遗传学教研室 郑州 450001
Author(s):
WEN Shenglan1XIE Shi1WANG Zhiwei1HE Nuo2LI Xingwu3CHEN Hui4
1)Department of Clinical Medicine, Zhengzhou University, Zhengzhou 4500522)Clinical Laboratory, Zhengzhou Central Hospital, Zhengzhou 4500073)Clinical Laboratory, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 4500524)Department of Cell Biology and Medical Genetics, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001
关键词:
性激素结合球蛋白(TAAAA)n多态性男性原发性不育河南汉族
Keywords:
sex hormonebinding globulin(TAAAA)n polymorphismidiopathic male infertilityHenan Han population
分类号:
R394.3
文献标志码:
A
摘要:
目的:探讨河南汉族男性原发性不育与性激素结合球蛋白(SHBG)基因启动子(TAAAA)n多态性的关联性。方法:分别收集288例河南汉族男性原发性不育患者和259例正常男性的精液或血液,酚氯仿法提取DNA,PCR扩增目的片段,检测Y染色体微缺失,对无缺失个体进行变性聚丙烯酰胺凝胶电泳、银染及生物测序分析SHBG基因启动子(TAAAA)n等位基因和基因型。结果:病例组Y染色体微缺失率为12.15%(35/288),对照组未检出。检测到(TAAAA)五核苷酸5~10次重复、6个等位基因及20种基因型。病例组与对照组(TAAAA)n多态性构成比较,差异有统计学意义(P<0.001)。其中,n=5、6、7、10时,2组间比较差异无统计学意义(P均>0.008);n=8为保护因素(P=0.002),n=9为危险因素(P<0.001);n≥9时增加了原发性不育的风险(P<0.001)。结论:河南汉族男性原发性不育与SHBG基因启动子(TAAAA)n多态性可能有一定关联性。
Abstract:
Aim: To investigate the association of the (TAAAA)n polymorphism in the sex hormonebinding globulin (SHBG) gene promoter with idiopathic male infertility in Henan Han population.Methods: Semen samples of 288 men with idiopathic infertility and blood samples of 259 normal controls were collected. Genomic DNA was extracted by phenolchloroform method and target fragment was amplified by PCR. The Ychromosome microdeletion was examined in all samples.The gene polymorphism was obtained by PAGE, silver staining method, and biological sequencing in the group without Ychromosome microdeletion.Results: Ychromosome microdeletion were detected in 12.15%(35/288) infertile men,while not found in controls. Six alleles containing (TAAAA) 5-10 repeats and 20 genotypes were presented in the subjects. The constituent ratio of (TAAAA)n polymorphism between the cases and controls was different(P<0.001).When n=5, 6, 7, 10,there was no significant difference(P>0.008).n=8 was a protective factor(P=0.002),and n=9 was a dangerous factor(P<0.001).n≥9 increased risk of infertility(P<0.001).Conclusion: There may be some correlation between the (TAAAA)n polymorphism in the SHBG gene promoter with idiopathic male infertility.

参考文献/References:

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更新日期/Last Update: 1900-01-01