[1]李霞,史惠蓉#,邓佑兴,等.沉默MACC1的表达对卵巢癌细胞系SKOV-3顺铂化疗敏感性的影响[J].郑州大学学报(医学版),2016,(01):92-94.
 LI Xia,SHI Huirong,DENG Youxing,et al.Effect of silencing MACC1 gene on cisplatin chemosensibility of ovarian cancer cell line SKOV3[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(01):92-94.
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沉默MACC1的表达对卵巢癌细胞系SKOV-3顺铂化疗敏感性的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年01期
页码:
92-94
栏目:
应用研究
出版日期:
2016-01-20

文章信息/Info

Title:
Effect of silencing MACC1 gene on cisplatin chemosensibility of ovarian cancer cell line SKOV3
作者:
李霞史惠蓉#邓佑兴张瑞涛
郑州大学第一附属医院妇产科;河南省高等学校临床医学重点学科开放实验室 郑州 450052
Author(s):
LI XiaSHI HuirongDENG YouxingZHANG Ruitao
KeyDisciplines Laboratory of ClinicalMedicine of Henan;Department of Gynecology and Obstetrics, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
结肠癌转移相关因子1RNA干扰顺铂敏感性卵巢癌SKOV3细胞
Keywords:
metastasisassociated in colon cancer 1RNA interferencecisplatin sensibilityovarian cancerSKOV3 cell
分类号:
R737.3
文献标志码:
A
摘要:
目的:探讨沉默结肠癌转移相关基因1(MACC1)对卵巢癌上皮性癌细胞SKOV3顺铂化疗敏感性的影响。方法:SKOV3细胞分为shRNA转染组(重组质粒psuperEGFPs3转染)、空质粒转染组(psuperEGFPneo转染)和对照组(未作转染),采用RTPCR法和Western blot法检测各组细胞MACC1 mRNA和蛋白的表达,MTT法检测细胞对顺铂的耐药性。结果:3组SKOV3细胞MACC1 mRNA和蛋白表达比较,差异有统计学意义(P<0.05),shRNA转染组较未转染组和空质粒组下降。shRNA转染组对顺铂的IC50较对照组和空质粒转染组下降(P<0.05)。结论:沉默MACC1的表达能增加卵巢癌SKOV3细胞对顺铂的敏感性。
Abstract:
Aim: To investigate the relationship of RNA interfering MACC1 and cisplatin sensibility of ovarian cancer cell line SKOV3.Methods: SKOV3 cells were divided into 3 groups:shRNA group(recombinant plasmid psuperEGFPs3 transfected SKOV3 cells), empty plasmid transfection group(psuperEGFPneo transfected SKOV3 cells) and control group (non transfected SKOV3 cells). RTPCR and Western blot were used to detect the expressions of MACC1 mRNA and protein. IC50 of cisplatin in different groups was determined by MTT assay.Results: The expressions of MACC1 mRNA and protein in the 3 groups were significantly different(P<0.05), and those of the shRNA transfection group were lower than those in the control group and empty plasmid group. The shRNA transfection group had lower IC50 than the control group and empty plasmid group(P<0.05).Conclusion: The sensitivity of SKOV3 cells to cisplatin could be enhanced by RNA interfering MACC1 gene.

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更新日期/Last Update: 1900-01-01