[1]王金兰,乐婷#,闫莹莹,等.阿托伐他汀对局灶性脑缺血大鼠脑组织中自噬及血管新生的影响[J].郑州大学学报(医学版),2016,(01):114-118.
 WANG Jinlan,YUE Ting,YAN Yingying,et al.Effect of atorvastatin on autophagy and angiogenesis in brain tissue of focal cerebral ischemia rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(01):114-118.
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阿托伐他汀对局灶性脑缺血大鼠脑组织中自噬及血管新生的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年01期
页码:
114-118
栏目:
应用研究
出版日期:
2016-01-20

文章信息/Info

Title:
Effect of atorvastatin on autophagy and angiogenesis in brain tissue of focal cerebral ischemia rats
作者:
王金兰乐婷#闫莹莹刘海燕
郑州大学第二附属医院神经内科 郑州450014
Author(s):
WANG JinlanYUE TingYAN YingyingLIU Haiyan
Department of Neurology,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014
关键词:
阿托伐他汀局灶性脑缺血大鼠自噬肌醇酶1LC3Ⅱ血管新生
Keywords:
atorvastatinfocal cerebral ischemiaratautophagyinositolrequiring enzyme 1LC3Ⅱangiogenesis
分类号:
R743
文献标志码:
A
摘要:
目的:观察阿托伐他汀对局灶性脑缺血大鼠脑组织中自噬及血管新生的影响。方法:雄性SD大鼠63只,随机分为假手术组、模型组、他汀组,每组21只。模型组和他汀组采用大脑中动脉栓塞法造模,假手术组不栓塞。术前1周,他汀组给予阿托伐他汀钙片4 mg/(kg·d)灌胃,其余两组给予等量生理盐水灌胃。术后24 h处死大鼠,TTC染色观察脑梗死面积,免疫组化法检测脑组织中CD105、IRE1的表达,RTPCR检测血管内皮细胞生长因子(VEGF) mRNA, Western blot法检测自噬蛋白LC3Ⅱ。结果:与假手术组相比,模型组及他汀组脑组织皮层及皮层下有明显梗死灶,缺血半暗带区CD105、IRE1、VEGF mRNA、LC3Ⅱ表达升高(P<0.05);他汀组上述变化较模型组更显著(P<0.05)。模型组、他汀组脑组织中VEGF mRNA与LC3Ⅱ表达有明显线性正相关关系(r=0.837、0.850,P均<0.05)。结论:阿托伐他汀可通过增强自噬,促进局灶性脑缺血血管新生。
Abstract:
Aim: To observe the effect of atorvastatin on autophagy and angiogenesis in brain tissue of focal cerebral ischemia rats.Methods: A total of 63 male SD rats were randomly allocated into sham operation group, model group and treatment group, and each group contained 21 rats.One week before surgery, the treatment group received a gavage of atorvastatin at 4 mg/(kg·d), and the other 2 groups were given the same amount of normal saline. All rats were killed 24 h after operation. The area of cerebral infarction was measured by TTC staining. The expressions of CD105 and IRE1 protein in brain tissue were detected by immunohistochemistry. The expression of VEGF mRNA was detected by RTPCR. The expression of LC3Ⅱprotein was detected by Western blot.Results: Compared with those of sham operation group, the cortex and subcortical brain tissue of model group and treatment group showed obvious infarction.The expressions of CD105,IRE1,VEGF mRNA and LC3Ⅱ in ischemic penumbra area in model group and treatment group were higher. Compared with those of the model group,the indexes mentioned above in treatment group were much higher(P<0.05). In model group and treatment group, the expression of LC3Ⅱ and that of VEGF mRNA had positive linear relationship (r=0.837,0.850,P<0.05).Conclusion: Atorvastatin can promote angiogenesis in focal cerebral ischemia tissue by enhancing autophagy.

参考文献/References:

[1]ZAITONE SA,ABOGRESHA NM.Rosuvastatin promotes angiogenesis and reverses isoproterenolinduced acute myocardial infarction in rats: role of iNOS and VEGF[J].Eur J Pharmacol,2012,691(1/3):134
[2]SABE AA,ELMADHUN NY,SADEK AA,et al.Differential effects of atorvastatin on autophagy in ischemic and nonischemic myocardium in Ossabaw swine with metabolic syndrome[J].J Thorac Cardiovasc Surg,2014,148(6):3172
[3]ZHANG J,YANG Z,XIE L,et al.Statins, autophagy and cancer metastasis[J].Int J Biochem Cell Biol,2013,45(3):745
[4]PARIHAR SP,GULER R,KHUTLANG R,et al.Statin therapy reduces the mycobacterium tuberculosis burden in human macrophages and in mice by enhancing autophagy and phagosome maturation[J].J Infect Dis,2014,209(5):754
[5]WEI YM,LI X,XU M,et al.Enhancement of autophagy by simvastatin through inhibition of Rac1mTOR signaling pathway in coronary arterial myocytes[J].Cell Physiol Biochem,2013,31(6):925
[6]肖卉.自噬及内质网应激在内皮细胞管型形成中的作用[D].长沙:中南大学,2013.
[7]付裕,李滢,杨方万,等.内质网应激诱导自噬的分子机制及意义[J].现代生物医学进展,2013,13(12):2393
[8]张爱梅.脑缺血后血管内皮生长因子的表达及保护作用研究[D].天津:天津医科大学,2004.
[9]张圣雪,邱龄,肖传实,等.阿托伐他汀对血管内皮细胞自噬作用中Beclin1和Map1lc3基因mRNA表达的影响[J].中西医结合心脑血管病杂志,2009,7(6):696
[10]张立沙.自噬和内质网应激在大鼠脑缺血预适应中的作用和药物干预[D].苏州:苏州大学,2009.

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更新日期/Last Update: 1900-01-01