[1]许雅娟△,张莹莹,罗晓华,等.孕妇血浆中胎儿特异性C21orf105、PLAC4 mRNA的检测*[J].郑州大学学报(医学版),2016,(02):200-204.
 XU Yajuan,ZHANG Yingying,LUO Xiaohua,et al.Plasma levels of fetal-specific C21orf105 and PLAC4 mRNA of pregnant women with trisomy 21 syndrome fetus[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(02):200-204.
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孕妇血浆中胎儿特异性C21orf105、PLAC4 mRNA的检测*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年02期
页码:
200-204
栏目:
应用研究
出版日期:
2016-03-20

文章信息/Info

Title:
Plasma levels of fetal-specific C21orf105 and PLAC4 mRNA of pregnant women with trisomy 21 syndrome fetus
作者:
许雅娟张莹莹罗晓华翟闪闪冉利敏任利单洪 腾
郑州大学第三附属医院产科 郑州 450052
Author(s):
XU YajuanZHANG YingyingLUO XiaohuaZHAI ShanshanRAN LiminREN LidanHONG Teng
Department of Obstetrics,the Third Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
21-三体综合征 C21orf105 PLAC4 血浆
Keywords:
trisomy 21 syndrome C21orf105 PLAC4 plasma
分类号:
R714.5
摘要:
目的:探讨孕妇血浆中胎儿特异性C21orf105、PLAC4 mRNA的稳定性及其表达量检测的临床价值。方法:①收集30例健康单胎妊娠妇女的血样,分为8组,其中4组分别在室温中放置0、6、24、72 h,余4组分别于4 ℃条件下放置0、6、24、72 h,然后采用实时荧光定量RT-PCR法检测C21orf105、PLAC4 mRNA。②收集健康单胎妊娠妇女的血样,其中经染色体核型分析证实胎儿为21-三体综合征的孕妇38例,胎儿染色体正常的孕妇40例,同法检测血浆C21orf105、PLAC4 mRNA。结果:30例孕妇血浆中均能检测到C21orf105、PLAC4 mRNA,孕妇血浆室温放置72 h后,C21orf105、PLAC4 mRNA无明显降解(P>0.05)。21-三体综合征胎儿孕妇血浆中C21orf105、PLACA4 mRNA表达量高于正常胎儿孕妇(P<0.001)。结论:母体血浆中C21orf105、PLAC4 mRNA很稳定,其检测有望成为21-三体综合征产前筛查的有效指标。
Abstract:
Aim: To explore the stability of the fetal-specific C21orf105 and PLAC4 mRNA and their expressions in the maternal plasma of pregnant women with trisomy 21 syndrome fetus.Methods: The plasma of 30 healthy and singleton pregnant women were collected and allocated in 8 groups, 4 groups were stored for 0,6,24 and 72 h at 4 ℃, and the other 4 groups were stored for 0,6,24 and 72 h at room temperature,respectively, then the expressions of C21orf105 and PLAC4 mRNA were detected using real-time RT-PCR.A total of 40 blood samples from normal pregnant women and 38 samples from trisomy 21 syndrome fetus pregnant women were collected to detect C21orf105 and PLAC4 mRNA in maternal plasma.Results: C21orf105 and PLAC4 mRNA could be detected in all the maternal plasma samples, even in maternal plasma samples stored at room temperature for 72 h, and there was no significant differences among the 8 groups(P>0.05).The mRNA expression levels of C21orf105 and PLAC4 in maternal plasma of the trisomy 21 syndrome pregnant women were higher than those of the normal controls(P<0.001).Conclusion: C21orf105 and PLAC4 mRNA in maternal plasma is stable enough to be amplified,and they are potential markers for prenatal screening of trisomy 21 syndrome.

参考文献/References:

[1] ZHANG YC,LIU HQ,CHEN XL,et al.Noninvasive prenatal diagnosis of down syndrome in samples from southwest chinese gravidas using pregnant plasma placental RNA allelic ratio[J].Genet Test Mol Biomarkers,2012,16(9):1051
[2] LI PQ,ZHANG J,FAN JH,et al.Development of noninvasive prenatal diagnosis of trisomy 21 by RT-MLPA with a new set of SNP markers[J].Arch Gynecol Obstet,2014,289(1):67
[3] NG EK,TSUI NB,LAM NY,et al.Presence of filterable and nonfilterable mRNA in the plasma of cancer patients and healthy individuals[J].Clin Chem,2002,48(8):1212
[4] GO AT,VISSER A,MULDERS MA,et al.C21ORF105,a chromosome 21-encoded mRNA,is not a discriminative marker gene for prediction of Down syndrome in maternal plasma[J].Prenat Diagn,2007,27(2):146
[5] 刘红英,杨利丽,厉锋,等.孕妇外周血中胎儿特异性mRNA的筛选及其临床意义[J].中华妇产科杂志,2011,46(9):655
[6] 程蔚蔚,赵欣荣,张华,等.孕妇血浆中胎儿游离mRNA检测在唐氏综合征产前诊断中的应用[J].中国实用妇科与产科杂志,2010(1):53
[7] BANZOLA I,RUSTERHOLZ C,ZANNONI L,et al.PLAC4 and β-HCG mRNA levels are not altered in the maternal circulation of pregnancies with trisomy 21[J].Prenat Diagn,2008,28(13):1262
[8] TSUI NB,AKOLEKAR R,CHIU RW,et al.Synergy of total PLAC4 RNA concentration and measurement of the RNA single-nucleotide polymorphism allelic ratio for the noninvasive prenatal detection of trisomy 21[J].Clin Chem,2010,56(1):73
[9] TSUI NB,WONG CS,CHOW KC,et al.Investigation of biological factors influencing the placental mRNA profile in maternal plasma[J].Prenat Diagn,2014,34(3):251
[10]OKAZAKI S,SEKIZAWA A,PURWOSUNU Y,et al.Measurement of mRNA of trophoblast-specific genes in cellular and plasma components of maternal blood[J].J Med Genet,2006,43(9):e47
[11]HEUNG MM,JIN SN,TSUI NB,et al.Placenta-derived fetal specific mRNA is more readily detectable in maternal plasma than in whole blood[J].PLoS One,2009,4(6):e5858
[12]NG EK,TSUI NB,LAU TK,et al.mRNA of placental origin is readily detectable in maternal plasma[J].Proc Natl Acad Sci U S A,2003,100(8):4748

备注/Memo

备注/Memo:
*河南省基础与前沿技术研究计划项目 132300410197
△女,1963年4月生,本科,主任医师,研究方向:围产医学,E-mail:cnzzzsl@163.com
更新日期/Last Update: 2016-03-20