[1]赵小建,张洁钰,杨 帆,等.柯萨奇病毒B3诱导的病毒性心肌炎C57BL/6J小鼠模型的建立[J].郑州大学学报(医学版),2016,(02):212-217.
 ZHAO Xiaojian,ZHANG Jieyu,YANG Fan,et al.Establishment of C57BL/6J mouse myocarditis model induced by coxsackievirus B3[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(02):212-217.
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柯萨奇病毒B3诱导的病毒性心肌炎C57BL/6J小鼠模型的建立()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年02期
页码:
212-217
栏目:
应用研究
出版日期:
2016-03-20

文章信息/Info

Title:
Establishment of C57BL/6J mouse myocarditis model induced by coxsackievirus B3
作者:
赵小建张洁钰杨 帆王 丽张真真唐 琳赵洛沙#
郑州大学第一附属医院心内科 郑州 450052
Author(s):
ZHAO XiaojianZHANG JieyuYANG FanWANG LiZHANG ZhenzhenTANG LinZHAO Luosha
Department of Cardiology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
柯萨奇病毒B3 病毒性心肌炎 C57BL/6J小鼠 动物模型
Keywords:
coxsackievirus B3 viral myocarditis C57BL/6J mouse animal model
分类号:
R373.2+3
摘要:
目的:建立由柯萨奇病毒B3(CVB3)Nancy株诱导的病毒性心肌炎(VMC)C57BL/6J小鼠模型。方法:4周龄、6周龄C57BL/6J小鼠各50只,每个年龄组随机选取15只作为对照(腹腔注射PBS),剩余35只腹腔接种含CVB3 Nancy株的病毒悬液(1×105 PFU/只),于感染后第14天取心脏,行 HE染色和CD3、CD45免疫组化染色,进行病理学评价,检测血清白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)水平,并做心肌组织中病毒滴度检测,采用Western blot法检测磷酸化AKT、IkBα、GSK3β的表达。结果:4周龄小鼠造模过程中有19只(54.3%)死亡; 与对照小鼠比较,模型小鼠心肌呈现弥漫性炎性细胞浸润和片状心肌细胞变性坏死, CD3、CD45阳性细胞弥漫浸润于心肌间质,血清IL-1β、TNF-α水平明显升高(P<0.05),心肌组织中磷酸化AKT、IkBα、GSK3β表达增高(P<0.05)。6周龄小鼠造模过程中有3只(8.6%)死亡,模型小鼠心肌组织中炎症反应轻于4周龄模型小鼠(P<0.05)。结论:成功建立了 VMC 的C57BL/6J小鼠模型, 4周龄C57BL/6J小鼠更适用于模型的建立。
Abstract:
Aim: To establish a C57BL/6J mouse model of myocarditis induced by coxsackievirus B3(CVB3).Methods: A total of 50 male mice with 4-week-age or 6-week-age were divided into two groups: the control(n=15)were injected via intraperiton with PBS, and the model group(n=35)were injected via intraperiton with 1×105 PFU CVB3. After 14 d, mice hearts were harvested for HE staining and CD3,CD45 immunohistochemistry staining,the serum levels of IL-1β,TNF-α were assayed by ELISA, the expressions of p-AKT,p-IkBα and p-GSK3β were detected by Western blot.Results: 4-week-age model group came out with a higher mortality(19/35,54.3%), HE staining showed that a great deal of inflammatory cells concentrated in the adjacent area around the myocardiocytes,which was consistent with the finding that many CD3,CD45 positive macrophages gathered in the necrosis area,TNF-α and IL-6 level were increased(P<0.05),p-AKT,p-IκBα and p-GSK3β protein expression increased(P<0.05). While the 6-week-age model group showed a lower mortality(3/35,8.6%),and the immune response to CVB3 infection was milder than 4-week-age model group(P<0.05).Conclusion: A C57BL/6J mouse model of myocarditis induced by CVB3 is successfully established,and 4-week-age mice are recommended for this model.

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备注/Memo

备注/Memo:
#通信作者,女,1956年4月生,硕士,主任医师,研究方向:心血管内科疾病,E-mail:zhaoluosha@126.com
更新日期/Last Update: 2016-03-20