[1]苏 纯)△,李巧云),范 梅),等.microRNA-151检测用于评价卵巢癌铂类药物疗效的临床价值[J].郑州大学学报(医学版),2016,(02):223-227.
 SU Chun),LI Qiaoyun),FAN Mei),et al.Clinical value of microRNA-151 in evaluating effect of platinum drugs on patients with ovarian cancer[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(02):223-227.
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microRNA-151检测用于评价卵巢癌铂类药物疗效的临床价值()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年02期
页码:
223-227
栏目:
应用研究
出版日期:
2016-03-20

文章信息/Info

Title:
Clinical value of microRNA-151 in evaluating effect of platinum drugs on patients with ovarian cancer
作者:
苏 纯1)△李巧云1)范 梅2)陆 林3)
1)郑州大学第五附属医院妇产科 郑州 450052
2)郑州大学第一附属医院超声科 郑州 450052
3)郑州大学第三附属医院放射科 郑州 450052
Author(s):
SU Chun1) LI Qiaoyun1) FAN Mei2) LU Lin3)
1)Department of Gynecology and Obstetrics, the Fifth Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
2)Department of Ultrasound, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
3)Department of Radiology, the Third Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
卵巢癌 化疗 microRNA Bcl-2
Keywords:
ovarian cancer chemotherapy microRNA Bcl-2
分类号:
R737.31
摘要:
目的:探讨microRNA检测用于评价卵巢癌患者铂类药物疗效的临床价值。方法:研究纳入接受顺铂化疗的初治卵巢癌患者88例,根据ATP-TCA结果分为敏感组(45例)和耐药组(43例)。microRNA微阵列分析2组患者血清microRNA表达的差异,并使用qRT-PCR加以验证。绘制ROC曲线,分析microRNA-151高表达与卵巢癌化疗不敏感现象的关系。体外培养SKOV3细胞并转染pcDNA3.1-microRNA-151或pcDNA3.1空质粒,使用3 μmol/L顺铂处理细胞48 h,计算细胞存活率,同时采用qRT-PCR及Western blot检测microRNA-151对细胞Bcl-2表达的影响。结果:与敏感组患者相比,耐药组患者中有5种microRNA表达上调,5种microRNA表达下调。microRNA-151水平的检测对于评价卵巢癌患者铂类药物疗效有临床价值(AUC=0.719, 95%CI=0.607~0.831),高microRNA-151水平的患者发生对铂类药物不敏感的风险较高。高表达microRNA-151可减弱SKOV3细胞对铂类药物的敏感性,提高Bcl-2的表达(P均<0.05)。结论:检测microRNA-151水平可以评估卵巢癌患者对铂类药物的敏感性。
Abstract:
Aim: To investigate the clinical value of microRNA-151 in evaluating effect of platinum drugs on patients with ovarian cancer.Methods: Eighty-eight ovarian cancer patients treated with platinum drugs were allocated into sensitive group and insensitive group according to the result of ATP-TCA test. MicroRNA microarray was used to detect the change of microRNA expression between two groups and the results was proven by qRT-PCR. The clinical value of detecting microRNA-151 was estimated by ROC curves. Furthermore, SKOV3 cells were cultured in vitro and transfected with pcDNA3.1-microRNA-151 or empty pcDNA3.1. Non-transfected SKOV3 cells and transfected SKOV3 cells were treated with 3 μmol/L cisplatin for 48 h and CCK-8 assay was used to calculate the viability of different cells. Finally, the Bcl-2 expression change induced by microRNA-151 was confirmed by qRT-PCR and Western blot.Results: Compared with sensitive group, in insensitive group,5 kinds of microRNA expression were decreased and 5 were increased.ROC curves supported that microRNA-151 had effectively clinical value to assess the sensitivity of ovarian cancer patients to platinum drugs(AUC=0.719, 95%CI=0.607-0.831). High-expressed microRNA-151 was a risk factor for ovarian cancer patients to induce resistance to platinum drugs; moreover, high-expressed microRNA-151 could induce the resistance of SKOV3 cells to platinum drugs and increase Bcl-2 expression(P<0.05).Conclusion: MicroRNA-151 could be used to evaluate the sensitivity of ovarian cancer patients to platinum drugs.

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备注/Memo

备注/Memo:
△女,1971年6月生,本科,副主任医师,研究方向:妇科肿瘤,E-mail:suchun5h@163.com
更新日期/Last Update: 2016-03-20