[1]王兴玲△,蔡鹏飞,张文娟,等.不同年龄IVF-ET患者卵丘颗粒细胞端粒长度与卵母细胞成熟度及妊娠结局的关系[J].郑州大学学报(医学版),2016,(02):233-236.
 WANG Xingling,CAI Pengfei,ZHANG Wenjuan,et al.Relationship of telomere length in cumulus cells with oocytes maturation and outcome of IVF-ET in patients with different ages[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(02):233-236.
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不同年龄IVF-ET患者卵丘颗粒细胞端粒长度与卵母细胞成熟度及妊娠结局的关系()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年02期
页码:
233-236
栏目:
应用研究
出版日期:
2016-03-20

文章信息/Info

Title:
Relationship of telomere length in cumulus cells with oocytes maturation and outcome of IVF-ET in patients with different ages
作者:
王兴玲蔡鹏飞张文娟肖雅琳
郑州大学第三附属医院生殖医学中心 郑州 450052
Author(s):
WANG Xingling CAI Pengfei ZHANG Wenjuan XIAO Yalin
Reproductive Center, the Third Affiliated Hospital,Zhengzhou University, Zhengzhou 450052
关键词:
体外受精-胚胎移植 卵丘颗粒细胞 端粒长度 卵母细胞成熟度
Keywords:
IVF-ET cumulus cell telomere length oocyte maturation
分类号:
R711.6
摘要:
目的:探讨体外受精-胚胎移植(IVF-ET)卵丘颗粒细胞的端粒长度与卵母细胞成熟度及妊娠结局的关系。方法:收集81例行体外受精-胚胎移植患者的卵丘颗粒细胞,根据患者的年龄,分为低龄(<35岁)组42例和高龄(≥35岁)组39例。应用qRT-PCR测量卵丘颗粒细胞端粒长度。结果:卵丘颗粒细胞端粒长度与年龄呈负相关(r=-0.267,P=0.021),低、高龄组内成熟卵母细胞的卵丘颗粒细胞端粒长度明显比未成熟卵母细胞的长(P<0.05)。高龄组妊娠者端粒长度较未妊娠者长(P<0.05)。结论:在IVF-ET中,卵丘颗粒细胞的相对端粒长度随年龄增长逐渐缩短,可反映卵母细胞的成熟度; 端粒长度缩短可能会影响妊娠结局。
Abstract:
Aim: To investigate the relationship of the relative telomere length in cumulus cells(CCs)with oocytes at different mature stages and the outcome of in vitro fertilization and embryo transfer(IVF-ET).Methods: Oocyte-cumulus complex samples were collected from 81 patients undergoing IVF-ET and CCs were manually separated. A total of 42 women(<35 years,the younger group)and 39 women(≥35 years, the older group)were collected. The oocyte maturation and the result of clinical pregnancy were recorded in different groups. DNA was extracted from CCs and assessed for telomere length by real-time quantitative PCR.Results: There were negative correlation between relative telomere length of CCs with the patients' age(r=-0.267, P=0.021). The relative telomere length of CCs was higher in mature oocytes than immature oocytes in both groups(P<0.05). There was a significantly higher telomere length of CCs in the pregnant subgroup than in the non-pregnant subgroup among the older group(P<0.05).Conclusion: In IVF-ET, the relative telomere length of CCs gradually shortened with age. The telomere length of CCs could reflect the degree of oocyte maturation and may be associated with pregnancy outcome in IVF-ET.

参考文献/References:

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备注/Memo

备注/Memo:
△女,1962年2月生,博士,主任医师,教授,研究方向:生殖内分泌与辅助生殖,E-mail:wangxingling123456@163.com
更新日期/Last Update: 2016-03-20