[1]张卫芳),孙 燕),张伟杰),等.TM4SF1在人乳腺癌细胞MCF-7和MDA-MB-231中的表达及其对细胞增殖、迁移能力的影响[J].郑州大学学报(医学版),2016,(02):240-244.
 ZHANG Weifang),SUN Yan),ZHANG Weijie),et al.Expression of TM4SF1 and its effect on proliferation and migration of human breast cancer cells MCF-7 and MDA-MB-231[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(02):240-244.
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TM4SF1在人乳腺癌细胞MCF-7和MDA-MB-231中的表达及其对细胞增殖、迁移能力的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年02期
页码:
240-244
栏目:
应用研究
出版日期:
2016-03-20

文章信息/Info

Title:
Expression of TM4SF1 and its effect on proliferation and migration of human breast cancer cells MCF-7 and MDA-MB-231
作者:
张卫芳1)孙 燕2)张伟杰1)朱 换1)王留兴1)#
1)郑州大学第一附属医院肿瘤科一病区 郑州 450052
2)中国医学科学院肿瘤医院肿瘤内科 北京 100021
Author(s):
ZHANG Weifang1) SUN Yan2) ZHANG Weijie1) ZHU Huan1) WANG Liuxing1)
1)Ward One of Oncology, the First Affiliated Hospital, Zhengzhou University,Zhengzhou 450052
2)Department of Oncology, Cancer Hospital, Chinese Academy of Medical Sciences, Beijing 100021
关键词:
TM4SF1 乳腺癌 转染 细胞增殖 迁移
Keywords:
TM4SF1 breast cancer transfection cell proliferation migration
分类号:
R737.9
摘要:
目的:探讨TM4SF1在人乳腺癌细胞MCF-7和MDA-MB-231中的表达情况以及靶向抑制TM4SF1基因后2种细胞的增殖、迁移能力的变化。方法:采用RT-PCR检测MCF-7和MDA-MB-231细胞中TM4SF1 mRNA表达情况,以正常乳腺上皮细胞MCF-10A的TM4SF1 mRNA表达作为参照; 应用TM4SF1 siRNA瞬时转染MCF-7、MDA-MB-231细胞,应用Western blot方法分析转染效果和TM4SF1蛋白表达情况; CCK-8法检测沉默TM4SF1前后2种细胞增殖能力的变化; 划痕实验检测细胞的迁移能力。结果:MCF-7细胞中TM4SF1 mRNA表达量为(1.159±0.218),MDA-MB-231细胞中表达量为(1.396±0.199),均高于MCF-10A细胞中的表达量(0.016±0.004)(P均<0.05); 且恶性程度较高的细胞MDA-MB-231高于低度恶性的MCF-7细胞(P<0.05)。特异性转染TM4SF1 siRNA后,该基因表达被抑制,同时MCF-7和MDA-MB-231细胞的体外迁移能力下降(P均<0.05),而细胞的增殖能力变化差异无统计学意义(P>0.05)。结论:TM4SF1在人乳腺癌细胞MCF-7和MDA-MB-231中高表达,并增强乳腺癌细胞的迁移能力。
Abstract:
Aim: To explore the expression of TM4SF1 mRNA in MCF-7 and MDA-MB-231 cells, and investigate its effect on the proliferation and migration when TM4SF1 was silenced.Methods: The expression of TM4SF1 mRNA in MCF-7 and MDA-MB-231 cells was determined by RT-PCR, and the results were compared with that of MCF-10A cells. RNA interference method was applied to transiently transfect siRNA targeting at TM4SF1 into MCF-7 and MDA-MB-231 cells, the transfection efficiency and the protein expression of TM4SF1 in MCF-7 and MDA-MB-231 cells were analyzed by Western blot, CCK-8 was used to measure the difference of cell proliferation when TM4SF1 was silenced,and the mobility was determined by the scratch test.Results: The expression of TM4SF1 mRNA in MCF-7 cells(1.159±0.218)and in MDA-MB-231 cells(1.396±0.199)were both significantly higher than the expression in MCF-10A cells(0.016±0.004)(P<0.05), and the expression of TM4SF1 mRNA in MDA-MB-231 cells was higher than that in MCF-7 cells(P<0.05). After the TM4SF1 siRNA was transfected into the cells,the TM4SF1 mRNA expression were significantly inhibited, while the capability of migration of the two cells were decreased(P<0.05), and the difference of cell proliferation had no significance(P>0.05).Conclusion: TM4SF1 is highly expressed in MCF-7 and MDA-MB-231 cells, and enhances the migration of the cells.

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备注/Memo

备注/Memo:
#通信作者,男,1952年2月生,教授,主任医师,研究方向:乳腺癌和前列腺癌的基础与临床,E-mail:wlx2246@126.com
更新日期/Last Update: 2016-03-20