[1]侯 英),白雪娟),梁 艳),等.结核分枝杆菌Rv1242蛋白抗原表位的预测*[J].郑州大学学报(医学版),2016,(03):345-347.
 HOU Ying),BAI Xuejuan),LIANG Yan),et al.Prediction of epitopes of Mycobacterium tuberculosis Rv1242 protein[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(03):345-347.
点击复制

结核分枝杆菌Rv1242蛋白抗原表位的预测*()
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年03期
页码:
345-347
栏目:
应用研究
出版日期:
2016-05-20

文章信息/Info

Title:
Prediction of epitopes of Mycobacterium tuberculosis Rv1242 protein
作者:
侯 英12)白雪娟2)梁 艳2)吴雪琼2)#
1)北京卫生职业学院医学技术系 北京 100053;
2)中国人民解放军第309医院全军结核病研究所; 全军结核病防治重点实验室; 结核病诊疗新技术北京市重点实验室 北京 100091
Author(s):
HOU Ying12)BAI Xuejuan2)LIANG Yan2)WU Xueqiong2)
1)Department of Medical Technology,Beijing Health Vocational College,Beijing 100053;
2)Institute for Tuberculosis Research, the 309th Hospital of Chinese PLA; Army Tuberculosis Prevention and Control Key Laboratory; Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Beijing100091
关键词:
抗原表位 Rv1242蛋白 二级结构 结核分枝杆菌
Keywords:
epitope Rv1242 protein secondary structure Mycobacterium tuberculosis
分类号:
R52
摘要:
目的:预测结核分枝杆菌Rv1242蛋白的抗原表位。方法:利用DNAStar软件包中Protean软件对Rv1242氨基酸序列进行分析,采用包括二级结构、亲水性、抗原性、表面可能性、柔韧性等多参数预测其二级结构及T细胞和B细胞抗原表位。结果:Rv1242蛋白具有丰富的二级结构和多处抗原指数较高的区段,有7个亲水性区域,含有B细胞抗原肽表位(可能在17-38、72-82、84-90、116-130、134-143位氨基酸残基或其附近),这些表位抗原性较好,都含有β转角和不规则卷曲结构,表面可能性和柔韧性都较大。该蛋白还含有T细胞抗原肽表位(可能在24-33、42-59、68-77、90-108位氨基酸残基或其附近)。结论:Rv1242蛋白可能是一个既有T细胞抗原表位、也有B细胞抗原表位的抗原,该研究为进一步研究该蛋白抗原表位及其应用奠定了基础。
Abstract:
Aim: To predict the epitopes of Mycobacterium tuberculosis Rv1242 protein.Methods: The amino acid sequence of Rv1242 protein was input and B cell and T cell epitopes were predicted using multi-parameters such as hydrophilicity,antigenicity,accessibility,flexibility,as well as the secondary structure by Protean software of DNAStar software package.Results: Rv1242 protein had rich secondary structure and multiple sections with higher antigenicity. There were 7 hydrophilic regions. There were potential B cell epitopes at 17-38,72-82,84-90,116-130,134-143 amino acid residues or nearby. These epitopes had better antigenicity, contained beta angle and irregular coil structure, and presented in the surface with larger probability and flexibility. There were also potential T cell epitopes at 24-33,42-59,68-77,90-108 amino acid residues or nearby.Conclusion: Mycobacterium tuberculosis Rv1242 is probably a protein antigen with both T and B cell epitopes, which will lay the foundation for its further study and application.

参考文献/References:

[1] DE SOUZA GA, LEVERSEN NA, MALEN H.Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway[J].J Proteomics,2011,75(2):502
[2] HIWA MÅLEN,TINASØFTELAND,TINASØFTELAND,et al.Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv[J].BMC Microbiol,2010,10:132
[3] 朱育菁,刘波,郑伟文,等.基因序列蛋白质结构分析软件Protean使用技术[J].福建农业生物技术通讯,2004,6(4):8
[4] 吴雪琼.新型结核病疫苗的研究现状与发展趋势[J].中国防痨杂志,2012,34(3):133
[5] 李松,王英.生物信息学在生命科学研究中的应用[J].热带医学杂志,2009,9(10):1218
[6] 黎明,于天飞.DNAStar软件在动物病毒研究中的应用实例[J].高师理科学刊,2010,30(3):61
[7] 白雪娟,赵亚静,梁艳,等.结核潜伏感染蛋白Rv1737c B细胞、CTL及Th表位预测与分析[J].实用临床医药杂志,2015,19(3):5
[8] 李海侠,毛旭虎.蛋白质抗原表位研究进展[J].微生物学免疫学进展,2007,35(1):54
[9] MCMURRY J,SBAI H,GENNARO ML,et al.Analyzing Mycobacterium tuberculosis proteomes for candidate vaccine epitopes[J].Tuberculosis(Edinb),2005,85(1/2):95

相似文献/References:

[1]李江英),白雪娟),梁 艳),等.应用DNAStar软件预测结核分枝杆菌Rv3812的抗原表位*[J].郑州大学学报(医学版),2016,(02):166.
 LI Jiangying),BAI Xuejuan),LIANG Yan),et al.Prediction of epitopes of Mycobacterium tuberculosis Rv3812 protein using DNAStar software[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(03):166.

备注/Memo

备注/Memo:
*国家重大传染病防治科技重大专项基金资助项目 2012ZX10003008002; 军队医学科技“十二·五”重点项目 BWS11J050; 北京市科技创新基地培育与发展工程专项 Z141107004414021; 北京高等学校青年英才计划项目 YETP1999
#通信作者,女,1963年7月生,博士,研究员,研究方向:新型疫苗、诊断技术和新药的研究,E-mail:wu-xueqiong@263.net
更新日期/Last Update: 2016-05-20