[1]张 敏,赵书君,朱 海,等.HDAC2 siRNA转染对卵巢癌ovcar3细胞中p53和乙酰化p53k320表达的影响*[J].郑州大学学报(医学版),2016,(03):372-375.
 ZHANG Min,ZHAO Shujun,ZHU Hai,et al.Effect of HDAC2 siRNA transfection on expression of p53 and acetylated p53k320 in ovarian cancer ovcar3 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(03):372-375.
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HDAC2 siRNA转染对卵巢癌ovcar3细胞中p53和乙酰化p53k320表达的影响*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年03期
页码:
372-375
栏目:
应用研究
出版日期:
2016-05-20

文章信息/Info

Title:
Effect of HDAC2 siRNA transfection on expression of p53 and acetylated p53k320 in ovarian cancer ovcar3 cells
作者:
张 敏赵书君朱 海胡晓静余亚南韩会会曹 蒙李红雨#
郑州大学第三附属医院妇产科 郑州 450052
Author(s):
ZHANG Min ZHAO Shujun ZHU Hai HU Xiaojing YU Yanan HAN HuihuiCAO MengLI Hongyu
Department of Gynecology and Obstetrics,the Third Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
组蛋白去乙酰化酶2 p53 乙酰化p53k320 ovcar3细胞株 卵巢肿瘤
Keywords:
histone deacetylase 2 p53 acetylated p53k320 ovcar3 cells line ovarian neoplasm
分类号:
R737.31
摘要:
目的:探讨靶向沉默组蛋白去乙酰化酶2(HDAC2)对人卵巢癌ovcar3细胞中p53乙酰化位点k320表达的影响。方法:将ovcar3细胞随机分为空白组、对照组和实验组3组,实验组以HDAC2 siRNA转染细胞,对照组转染对照siRNA,空白组不处理,分别采用实时荧光定量PCR和Western blot方法检测3组细胞中HDAC2 mRNA和蛋白的表达,Western blot 检测3组细胞中p53、乙酰化p53k320蛋白的表达。结果:与空白组及对照组相比,实验组HDAC2 mRNA和蛋白的表达降低(P<0.05),p53蛋白和乙酰化p53k320蛋白表达均升高(P<0.05)。结论:下调HDAC2的表达可使p53和乙酰化p53k320表达增加。
Abstract:
Aim: To investigate the effect of targeted silencing histone deacetylase 2(HDAC2)on expression of p53 and acetylation p53k320 in human ovarian cancer cell line ovcar3.Methods: ovcar3 cells were divided into 3 groups. HDAC2 siRNA was used to transfected into the ovcar3 cells in experimental group, and the cells transfected with control siRNA(control siRNA group)and the cells without treatment(control group)were the control. Real Time-PCR and Western blot was used to detect the expression of HDAC2 mRNA and protein; the expression of p53 and acethylased p53k320 were examined by Western blot.Results: Compared with control group and control siRNA group,the expressions of HDAC2 mRNA and protein in experimental group were decreased(P<0.05), and the expressions of p53 and acethylased p53k320 protein were increased(P<0.05).Conclusion: Downregulating the expression of HDAC2 could increase the expressions of p53 and acethylased p53k320.

参考文献/References:

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备注/Memo

备注/Memo:
*郑州市科学技术局2015普通科研项目 153PKJGG165
#通信作者,女,1964年9月生,博士,教授,研究方向:妇科肿瘤,E-mail:lihongyu99@qq.com
更新日期/Last Update: 2016-05-20