[1]王兴玲△,张文娟,蔡鹏飞,等.卵泡液中游离DNA含量与卵母细胞成熟度及胚胎质量的关系[J].郑州大学学报(医学版),2016,(03):375-378.
 WANG Xingling,ZHANG Wenjuan,CAI Pengfei,et al.Relationship between content of cell-free DNA and oocyte maturation and embryo quality in follicular fluid[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(03):375-378.
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卵泡液中游离DNA含量与卵母细胞成熟度及胚胎质量的关系()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年03期
页码:
375-378
栏目:
应用研究
出版日期:
2016-05-20

文章信息/Info

Title:
Relationship between content of cell-free DNA and oocyte maturation and embryo quality in follicular fluid
作者:
王兴玲张文娟 蔡鹏飞肖雅琳
郑州大学第三附属医院生殖中心 郑州 450052
Author(s):
WANG XinglingZHANG WenjuanCAI PengfeiXIAO Yalin
Reproductive Center, the Third Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
游离DNA 成熟卵泡液 未成熟卵泡液 卵母细胞成熟度 胚胎质量
Keywords:
cell-free DNA mature follicular fluid immature follicular fluid oocyte maturation embryo quality
分类号:
R711.6
摘要:
目的:探讨卵泡液中游离DNA含量与卵母细胞成熟度及胚胎质量的关系。方法:共收集了87位患者的成熟与未成熟卵泡液。以年龄30岁为界,定义年龄<30岁的为A组,年龄≥30岁的为B组。使用DNA手工提取法(改良酚-氯仿-异丙醇法)进行卵泡液中游离DNA的提取并检测。结果:成熟卵泡液组中游离DNA含量[(1 168.4±81.1)μg/L]低于未成熟卵泡液组[(1 875.6±95.7)μg/L],差异有统计学意义(P<0.001)。A组可移植胚胎数目[(5.6±2.7)个]、优质胚胎数目[(3.9±1.8)个]高于B组[(4.1±2.5)和(2.5±1.6)个],A组成熟卵泡液中游离DNA含量[(1 129.4±78.6)μg/L]低于B组[(1 214.2±56.8)μg/L],差异均有统计学意义(P<0.001)。结论:游离DNA含量可能是评价卵泡细胞成熟度以及预测胚胎质量的指标。
Abstract:
Aim: To explore the relationship between the content of cell-free DNA and oocyte maturation and embryo quality in follicular fluid.Methods: The mature and immature follicular fluid of 87 patients were collected. Patients with age<30 years were group A(n=47), and patients with age≥30 years were group B(n=40). The concentration of cell-free DNA was detected by manual extraction(modified phenol-chloroform-isoamyl alcohol extraction).Results: The content of cell-free DNA in the mature follicular fluid group[(1 168.4±81.1)μg/L)] was significantly lower than that in immature follicular fluid group [(1 875.6±95.7)μg/L)](P<0.001). The patients' partial parameters such as the number of embryo numbers(5.6±2.7), good quality embryo number(3.9±1.8)in group A were significantly higher than the patients in group B[(4.1±2.5)and(2.5±1.6)]. But the content of cell-free DNA in group A [(1 129.4±78.6)μg/L)] was lower than that in group B[(1 214.2±56.8)μg/L)](P<0.001).Conclusion: The content of cell-free DNA can be used as a biological indicator to evaluate oocyte maturation, and it may also be an indicator to predict the quality of embryos.

参考文献/References:

[1] SCALICI E,TRAVER S,MOLINARI N,et al.Cell-free DNA in human follicular fluid as a biomarker of embryo quality[J].Hum Reprod,2014,29(12):2661
[2] 张丽丽,王海英,张仁礼,等.人卵泡液hCG水平与卵母细胞成熟及早期胚胎发育的关系[J].南方医科大学学报,2014,34(2):260
[3] 汪淑娟,高志英,卢彦平,等.高龄孕妇胎儿染色体非整倍体异常的早孕期检测[J].南方医科大学学报,2014,34(5):655
[4] CHEN K,ZHANG H,ZHANG LN,et al.Value of circulating cell-free DNA in diagnosis of hepatocelluar carcinoma[J].World J Gastroenterol,2013,19(20):3143
[5] LAMBERT-MESSERLIAN G,KLOZA EM,WILLIAMS IJ,et al.Maternal plasma DNA testing for aneuploidy in pregnancies achieved by assisted reproductive technologies[J].Genet Med,2014,16(5):419
[6] TRAVER S,ASSOU S,SCALICI E,et al.Cell-free nucleic acids as non-invasive biomarkers of gynecological cancers, ovarian, endometrial and obstetric disorders and fetal aneuploidy[J].Hum Reprod Update,2014,20(6):905
[7] 谈际范,何思锐,罗璐,等.既往自然流产次数与患者第3日胚胎质量的关系研究[J].生殖与避孕,2015,35(11):811
[8] LIU L,ZHOU F,LIN XN,et al.Recurrent IVF failure is associated with elevated progesterone on the day of hCG administration[J].Eur J Obstet Gynecol Reprod Biol,2013,171(1):78
[9] 刘晓莉,邹丽颖,陈奕,等.孕产妇年龄对妊娠及分娩结局的影响[J].中华医学杂志,2014,94(25):1984
[10]张静,孙博,卜志勤,等.年龄对长方案体外受精-胚胎移植助孕的影响[J].国际生殖健康/计划生育杂志,2014,33(3):239
[11]KOSTYUK S,SMIRNOVA T,KAMENEVA L,et al.GC-rich extracellular DNA induces oxidative stress, double-strand DNA breaks, and DNA damage response in human adipose-derived mesenchymal stem cells[J].Oxid Med Cell Longev,2015,2015:782123
[12]HAZOUT A. Cell-free DNA as a therapeutic target for female infertility and diagnostic marker:WO2014/020564 A1[P].2014-06-02.

备注/Memo

备注/Memo:
△女,1962年2月生,博士,教授,主任医师,研究方向:生殖内分泌与辅助生殖技术,E-mail:wangxingling2013@163.com
更新日期/Last Update: 2016-05-20