[1]朱相展),张彦婷),杨 露),等.β-胡萝卜素对食管鳞癌EC1细胞增殖、凋亡、迁移及细胞周期的影响[J].郑州大学学报(医学版),2016,(04):437-441.
 ZHU Xiangzhan),ZHANG Yanting),YANG Lu),et al.Effects of β-carotene on proliferation, apoptosis, migration and cell cycle of EC1 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(04):437-441.
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β-胡萝卜素对食管鳞癌EC1细胞增殖、凋亡、迁移及细胞周期的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年04期
页码:
437-441
栏目:
食管癌研究
出版日期:
2016-07-20

文章信息/Info

Title:
Effects of β-carotene on proliferation, apoptosis, migration and cell cycle of EC1 cells
作者:
朱相展1)张彦婷1)杨 露1)张楠楠2)李庆华1)马珊珊1)张 琨1)薛乐勋1)关方霞12)#
1)郑州大学生命科学学院 郑州 450001;
2)郑州大学第一附属医院干细胞实验室 郑州 450052
Author(s):
ZHU Xiangzhan1)ZHANG Yanting1)YANG Lu1) ZHANG Nannan2)LI Qinghua1)MA Shanshan1)ZHANG Kun1)XUE Lexun1)GUAN Fangxia12)
1)College of Life Sciences, Zhengzhou University, Zhengzhou 450001;
2)Stem Cell Laboratory,the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
β-胡萝卜素 食管鳞癌 Cav-1/AKT/NF-κB 凋亡
Keywords:
β-carotene esophageal squamous cell carcinoma Cav-1/AKT/NF-κB apoptosis
分类号:
R735.1
摘要:
目的:观察β-胡萝卜素对食管鳞癌EC1细胞增殖、凋亡、迁移及细胞周期的影响。方法:用不同浓度(1、5、10、20、30、50 μmol/L)β-胡萝卜素分别处理EC1细胞12、24、36、48、60、72 h后,CCK-8法检测β-胡萝卜素对EC1细胞增殖率的影响,筛选β-胡萝卜素的最佳处理时间及浓度; 随后,在此条件下将EC1细胞分为3组(阴性对照组、空白对照组和实验组),采用Annexin V-FITC/PI双染法检测细胞凋亡,流式细胞仪检测细胞周期的变化,Transwell迁移实验检测细胞迁移能力的变化,Western blot检测小窝蛋白1(Cav-1)、p-Akt、p-NF-κB、Bcl-2、Caspase-3、E-cadherin蛋白表达水平的变化。结果:经筛选,选用50 μmol/L β-胡萝卜素作用EC1细胞48 h进行后续实验。与阴性对照组和空白对照组相比,实验组EC1细胞凋亡率显著提高(P<0.001),G0/G1期细胞比例增加(P<0.001),而S期细胞比例降低(P<0.001),发生迁移的细胞数量减少(P<0.001),且细胞黏附因子E-cadherin的表达显著增加(P<0.001),Cav-1及AKT信号通路关键蛋白p-Akt、p-NF-κB、Bcl-2表达量明显下调,而Caspase-3的表达被激活(P<0.001)。结论:β-胡萝卜素能够有效促进EC1细胞的凋亡,推测可能是通过抑制Cav-1介导的AKT/NF-κB信号通路发挥作用。
Abstract:
Aim: To investigate the effects of β-carotene on the proliferation, apoptosis, migration and cell cycle of human esophageal cancer EC1 cells and to study its related molecular mechanism.Methods: After treatment with different concentrations(1, 5, 10, 20, 30 and 50 μmol/L)of β-carotene for 12, 24, 36, 48, 60 and 72 h, the proliferation rate was tested by CCK-8 assay to determine the optimal time and concentration,EC1 cells were allocated into 3 groups: negative control group, blank control group and treatment group. Then the cell cycle and apoptosis were detected by FCM and Annexin V-FITC/PI staining respectively; the cell migration was measured by Transwell assay; effects of the β-carotene on protein expressions of Cav-1, p-Akt, p-NF-κB, Bcl-2, Caspase-3 and E-cadherin were detected by Western blot.Results: After screening, the optimal dose of β-carotene and treatment time for EC1 cells were determined as 50 μmol/L and 48 h. After treatment with β-carotene,compared with negative control group and blank control group,EC1 cells in treatment group had a higher apoptosis rate(P<0.001),the cell cycle changed significantly with an increase in G0/G1 phase cells(P<0.001)and a decrease in S phase cells(P<0.001),and the number of migrating cells was significantly less(P<0.001). Meanwhile, the expression of E-cadherin in the treatment group was increased significantly(P<0.001),p-Akt, p-NF-κB and Bcl-2 decreased,and Caspase-3 was activated(P<0.001).Conclusion: β-carotene can effectively induce apoptosis of EC1 cells, which may be mediated by Cav-1 via AKT/NF-κB signaling pathway.

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目 81071008,81471306,U1404313; 河南省科技创新人才计划 154200510008; 河南省高校科技创新团队支持计划 15IRTSTHN022; 河南省产学研合作项目 142107000008
#通信作者,女,1969年2月生,博士,教授,研究方向:生物医学,E-mail:guanfangxia@126.com
更新日期/Last Update: 2016-07-20