[1]宋及时),马珊珊)#,孟 楠),等.rhMG53蛋白联合hUC-MSCs移植对阿尔茨海默鼠的治疗作用[J].郑州大学学报(医学版),2016,(04):442-446.
 SONG Jishi),MA Shanshan),MENG Nan),et al.Therapeutic effects of rhMG53 combination with hUC-MSCs transplantation on Alzheimer's disease mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(04):442-446.
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rhMG53蛋白联合hUC-MSCs移植对阿尔茨海默鼠的治疗作用()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年04期
页码:
442-446
栏目:
论著
出版日期:
2016-07-20

文章信息/Info

Title:
Therapeutic effects of rhMG53 combination with hUC-MSCs transplantation on Alzheimer's disease mice
作者:
宋及时1)马珊珊1)#孟 楠2)王欣欣2)姚 宁3)朱相展3)程 康1)杨 波2)关方霞12)#
1)郑州大学生命科学学院干细胞研究室 郑州 450001;
2)郑州大学第一附属医院干细胞研究室 郑州 450052;
3)中美(河南)荷美尔肿瘤研究院 郑州 450000
Author(s):
SONG Jishi1) MA Shanshan1) MENG Nan2) WANG Xinxin2) YAO Ning3) ZHU Xiangzhan3) CHENG Kang1) YANG Bo2) GUAN Fangxia12)
1)Stem Cell Laboratory, School of Life Sciences, Zhengzhou University, Zhengzhou 450001;
2)Stem Cell Laboratory, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052;
3)China-US(Henan)Hormel Cancer Institute,Zhengzhou 450000
关键词:
rhMG53 阿尔茨海默病 间充质干细胞 干细胞移植 小鼠
Keywords:
rhMG53 Alzheimer's disease mesenchymal stem cell stem cell transplantation mouse
分类号:
R592
摘要:
目的:研究rhMG53蛋白联合人脐带间充质干细胞(hUC-MSCs)移植对阿尔茨海默模型鼠(APP转基因小鼠)的治疗作用。方法:40只APP+小鼠随机分为APP+组、hUC-MSCs组、rhMG53组和rhMG53联合hUC-MSCs组(联合组),分组处理1周后采用免疫组化法检测小鼠脑内hUC-MSCs的迁移率,3周后采用Morris水迷宫测试各组小鼠的学习和记忆能力,同时检测小鼠血清中SOD的活性和MDA的含量,并采用qRT-PCR和Western blot检测小鼠脑组织中衰老相关基因(sirt2、pCNA、p16、p53)的表达。结果:APP+组和rhMG53组未见hUC-MSCs迁移,hUC-MSCs组和联合组存在hUC-MSCs迁移,且联合组迁移细胞更多(P<0.05)。hUC-MSCs移植可以使APP+小鼠的逃避潜伏期缩短,穿越平台次数增加以及平台所在象限停留时间增加(P<0.05); hUC-MSCs移植和rhMG53均可使APP+小鼠血清SOD活性增加,MDA含量下降,且二者联用对MDA的影响有协同作用(P<0.05); hUC-MSCs移植和rhMG53均可使APP+小鼠脑组织sirt2和pCNA mRNA和蛋白的相对表达量升高,且二者联用对多数衰老相关基因表达的影响有交互作用(P<0.05)。结论:外源rhMG53蛋白可以提高小鼠脑内hUC-MSCs的迁移,促进hUC-MSCs对阿尔茨海默鼠的治疗作用。
Abstract:
Aim: To research the effects of rhMG53 combination with hUC-MSCs transplantation on Alzheimer's disease mice.Methods: Forty APP+ mice were randomly allocated into four groups: APP+ group, hUC-MSCs group, rhMG53 group and rhMG53 combined with hUC-MSCs group. The migration of hUC-MSCs was quantified by immunohistochemistry at one week after transplantation. And the learning and memory ability was measured by Morris water maze test at three weeks after transplantation. The activity of superoxide dismutase(SOD)and the concentration of malonaldehyde(MDA)in the serum were also quantified. qRT-PCR and Western blot were used to detect the expressions of senescence related factors(sirt2,pCNA,p16,p53).Results: No migration of hUC-MSCs was found in APP+ group or rhMG53 group, while it was observed in the hUC-MSCs group and the combined group, and the amount of migration cells was higher in the combined group(P<0.05). hUC-MSCs transplantation decreased the escaping latency, increased the times of crossing platform and the time spent in the platform quadrant(P<0.05). hUC-MSCs transplantation and rhMG53 could both increase the activity of SOD and decrease the content of MDA in the serum of APP+ mice, and rhMG53 combined with hUC-MSCs transplantation had synergistic effect on MDA(P<0.05). hUC-MSCs transplantation and rhMG53 could both increase the expressions of sirt2 and pCNA mRNA and protein in the brain tissue of APP+ mice, and rhMG53 combined with hUC-MSCs transplantation had synergistic effect on the expression of most aging genes(P<0.05).Conclusion: rhMG53 can improve the migration of hUC-MSCs in brain tissue of APP+ mice, and promote the therapeutic effect of hUC-MSCs on APP+ mice.

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目 81471306,U1404313; 河南省科技创新人才计划 154200510008; 河南省高校科技创新团队支持计划 15IRTSTHN022; 河南省产学研合作项目 142107000008
#通信作者:关方霞,女,1969年2月生,博士,教授,研究方向:干细胞与再生医学, E-mail: guanfangxia@126.com; 马珊珊,女,1984年5月生,博士,副教授,研究方向:干细胞生物学特性分析,E-mail: mashanshan84@163.com
更新日期/Last Update: 2016-07-20