[1]刘雪亚),王 平),李 杰),等.大气细颗粒物不同成分对A549细胞遗传毒性的影响[J].郑州大学学报(医学版),2016,(04):482-486.
 LIU Xueya),WANG Ping),LI Jie),et al.Effects of different fractions of air particulate matter on genotoxicity in A549 cell line[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(04):482-486.
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大气细颗粒物不同成分对A549细胞遗传毒性的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年04期
页码:
482-486
栏目:
论著
出版日期:
2016-07-20

文章信息/Info

Title:
Effects of different fractions of air particulate matter on genotoxicity in A549 cell line
作者:
刘雪亚1)王 平2)李 杰2)李 萍1)卫军华3)高祎楠3)张国俊1)#
1)郑州大学第一附属医院呼吸内科 郑州 450052;
2)河南省职业病防治研究院毒理室 郑州 450052;
3)郑州大学化学与分子工程学院 郑州 450001
Author(s):
LIU Xueya1)WANG Ping2)LI Jie2)LI Ping1)WEI Junhua3)GAO Yinan3)ZHANG Guojun1)
1)Department of Respiration Diseases,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052;
2)Department of Toxicology, Henan Institute of Occupational Diseases Prevention and Control,Zhengzhou 450052;
3)College of Chemistry and Molecular Engineering,Zhengzhou University,Zhengzhou 450001
关键词:
细颗粒物 A549细胞 增殖 微核试验
Keywords:
particulate matter A549 cell proliferation micronuclei test
分类号:
R563.9
摘要:
目的:研究大气细颗粒物(PM2.5)不同成分对人肺腺癌Ⅱ型肺泡上皮细胞(A549)遗传毒性的影响,探索PM2.5暴露的细胞毒性机制及发病机制, 为开展河南地区PM2.5的健康危害评估提供实验依据。方法:于郑州市采集并处理PM2.5非水溶性成分和水溶性成分,分为PM2.5非水溶性和水溶性成分不同浓度(50、100、200、400、800 mg/L)染毒组和对照组,采用CCK-8比色法测量A549细胞在染毒后6、12、24、48和72 h的细胞存活率,微核实验检测各个浓度染毒24 h后细胞染色体损伤情况。结果:PM2.5非水溶性和水溶性成分均能抑制A549细胞增殖(P<0.05)。染毒24 h 时800 mg/L的大气PM2.5非水溶性成分或染毒48 h 时800 mg/L的大气PM2.5水溶性成分作用下细胞存活率最低。PM2.5非水溶性成分和水溶性成分均能诱导A549细胞染色体损伤,与对照组相比,实验组微核率明显升高(P<0.05),非水溶成分致细胞微核率较高(P<0.05)。结论:PM2.5能抑制A549细胞增殖,导致A549细胞染色体损伤。非水溶成分的影响可能更大。
Abstract:
Aim: To explore the effects of different fractions of air particulate matter(PM2.5)on genotoxicity in A549 cell line,to investigate the mechanism of cytotoxic and pathogenesis induced by particulate matter, and to provide experimental basis for the health hazard assessment of PM2.5 in Henan.Methods: Water-insoluble fractions and water-soluble fractions of PM2.5 were collected and processed in Zhengzhou. Then divided into PM2.5(50,100,200,400,800 mg /L)group(experimental groups)and control group. Finally the CCK-8 colorimetric method was used to measure the cytotoxicity in A549 cells after 6, 12, 24, 48 and 72 h, while micronucli test was adopted to detect the chromosome damage after 24 h.Results: Both water-insoluble fractions and water-soluble fractions of PM2.5 could inhibit the proliferation of A549 cells(P<0.05).And cell viability of A549 cells was the lowest after being treated by 800 mg/L PM2.5 water-insoluble fractions for 24 h,and for 800 mg/L PM2.5 water-soluble fractions treated for 48 h were lowest. Both water-insoluble fractions and water-soluble fractions of PM2.5 could induce chromosome damage of A549 cells. The micronucleus rate of experimental groups was higher than that of control groups(P<0.05). The micronucleus rate in A549 cells treated by water-soluble fractions was lower than that in A549 cells treated by water-insoluble fractions at the same concentration(P<0.05).Conclusion: PM2.5 could inhibit A549 cells proliferation, and result in chromosome damage; the water-insoluble fractions of PM2.5 may have greater effects.

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备注/Memo

备注/Memo:
*河南省医学科技攻关计划项目 201401005
#通信作者,男,1966年10月生,博士,主任医师,教授,研究方向:呼吸系统疾病基础与临床,E-mail:zlgj-001@126.com
更新日期/Last Update: 2016-07-20