[1]周文平)△,张 辉),张金盈).MEF2A RNA干扰对小鼠主动脉内皮细胞MEF2A表达及细胞间黏附分子-1、血管细胞黏附分子-1表达的 影响[J].郑州大学学报(医学版),2016,(04):502-505.
 ZHOU Wenping),ZHANG Hui),ZHANG Jinying).Effects of RNA interference of myocyte enhancer factor 2A on expressions of MEF2A, ICAM-1, and VCAM-1 in mice aortic endothelial cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(04):502-505.
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MEF2A RNA干扰对小鼠主动脉内皮细胞MEF2A表达及细胞间黏附分子-1、血管细胞黏附分子-1表达的 影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年04期
页码:
502-505
栏目:
应用研究
出版日期:
2016-07-20

文章信息/Info

Title:
Effects of RNA interference of myocyte enhancer factor 2A on expressions of MEF2A, ICAM-1, and VCAM-1 in mice aortic endothelial cells
作者:
周文平1)△张 辉2)张金盈2)
1)郑州大学第三附属医院心内科 郑州 450052;
2)郑州大学第一附属医院心内科 郑州 450052
Author(s):
ZHOU Wenping1)ZHANG Hui2)ZHANG Jinying2)
1)Department of Cardiovascular Medicine, the Third Affiliated Hospital, Zhengzhou University, Zhengzhou 450052;
2)Department of Cardiovascular Medicine, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
小鼠 肌细胞增强因子2A RNA干扰 ICAM-1 VCAM-1
Keywords:
mouse myocyte enhancer factor 2A RNA interference ICAM-1 VCAM-1
分类号:
R541.4
摘要:
目的:观察肌细胞增强因子2A(MEF2A)RNA干扰对小鼠主动脉内皮细胞MEF2A及细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)表达的影响。方法:培养小鼠主动脉内皮细胞,构建MEF2A RNA干扰慢病毒或者阴性对照慢病毒(NC)并转染小鼠主动脉内皮细胞。实验设对照组(不加慢病毒)、NC组和MEF2A RNA干扰组3组,应用ELISA和RT-PCR法分别检测MEF2A及ICAM-1、VCAM-1蛋白及mRNA的表达水平。结果:ICAM-1在对照组、NC组和MEF2A RNA干扰组上清液中的表达量分别为(1.133±0.182)、(1.267±0.115)、(2.249±0.185)μg/L; VCAM-1在对照组、NC组和MEF2A RNA干扰组上清液中的表达量分别为(2.382±0.204)、(2.253±0.281)、(5.077±0.198)μg/L。ICAM-1、VCAM-1在上清液中的水平及mRNA表达水平,NC组与对照组相比,差异无统计学意义(P>0.05); MEF2A RNA干扰组与对照组和NC组相比均升高(P<0.05)。结论:慢病毒介导的MEF2A RNA干扰可抑制小鼠主动脉内皮细胞MEF2A活性及mRNA的表达,并可促进血管内ICAM-1、VCAM-1蛋白及mRNA的表达。
Abstract:
Aim: To identify the effects of RNA interference of myocyte enhancer factor 2A(MEF2A)on the expressions of MEF2A and relative inflammatory genes ICAM-1 and VCAM-1 in mice aortic endothelial cells.Methods: The aortic endothelial cells in mice were cultivated, then MEF2A lentivirus RNA interference or negative control lentivirus(NC)were built and transfected aortic endothelial cells in mice. The cells were allocated into control group(without lentivirus), NC group, and MEF2A RNA interference group. MEF2A,ICAM-1, and VCAM-1 expressions were detected by ELISA, and mRNA levels of MEF2A,ICAM-1, and VCAM-1 were further examined by RT-PCR.Results: In the control group, NC group, and MEF2A RNA interference group,ICAM-1 expression in the supernatant fluid were(1.133±0.182),(1.267±0.115),and(2.249±0.185)μg/L, VCAM-1 expression in the supernatant fluid were(2.382±0.204),(2.253±0.281),and(5.077±0.198)μg/L. There was no significant difference in ICAM-1 or VCAM-1 expression in the supernatant fluid between NC group and the control group(P>0.05).When MEF2A RNA interference group was compared with the control group and NC group, there were significant differences(P<0.05).Conclusion: Lentivirus-mediated RNA interference of MEF2A could inhibit the expression of MEF2A and promote intravascular ICAM-1, VCAM-1 activity mRNA expression.

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备注/Memo

备注/Memo:
*河南省高校科技创新团队基金资助项目 14IRTSTHN018
△男,1967年12月生,博士,副主任医师,研究方向:冠心病,E-mail:wpzhou1212@126.com
更新日期/Last Update: 2016-07-20