[1]赵莘瑜△,苏 刚.早期生长反应因子对U87MG细胞Aβ40表达的影响[J].郑州大学学报(医学版),2016,(04):513-516.
 ZHAO Xinyu,SU Gang.Effect of early growth response 1 on expression of Αβ40 in U87MG cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(04):513-516.
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早期生长反应因子对U87MG细胞Aβ40表达的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年04期
页码:
513-516
栏目:
应用研究
出版日期:
2016-07-20

文章信息/Info

Title:
Effect of early growth response 1 on expression of Αβ40 in U87MG cells
作者:
赵莘瑜苏 刚
郑州大学第一附属医院神经内科 郑州 450052
Author(s):
ZHAO XinyuSU Gang
Department of Neurology, the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
阿尔茨海默病 Aβ40 EGR1 U87MG细胞
Keywords:
Alzheimer's disease beta amyloid protein 40 early growth response 1 U87MG cells
分类号:
R742
摘要:
目的:研究早期生长因子EGR1对U87MG细胞Aβ40表达的影响。方法:构建EGR1表达质粒并转染U87MG(实验组),以转染空白质粒的U87MG细胞作为对照。采用qRT-PCR法检测两组EGR1 mRNA的表达; 收集细胞培养液用ELISA法检测Aβ40的水平; 提取总蛋白用蛋白印迹法检测BACE1和PS1蛋白的表达。结果:质粒成功构建并表达。实验组EGR1 mRNA表达水平较对照组明显增高(P<0.001); 实验组细胞培养液中Aβ40水平较对照组增高(P<0.001); 实验组BACE1蛋白的表达较对照组增加(P<0.001); 两组PS1蛋白的表达差异无统计学意义(P=0.367)。结论:EGR1在体外可能使Aβ40表达增加,且可能与上调BACE1有关。
Abstract:
Aim: To research the effect of early growth response 1(EGR1)on the expression of beta amyloid protein 40(Aβ40)U87MG cells.Methods: To construct overexpression plasmid of CMV-EGFP-EGR1-Kanamycin and transfect U87MG cells as the experimental group; the control group cells were transfected by the plasmids of CMV-EGFP-Kanamycin. The expression of EGR1 mRNA was detected by qRT-PCR. The culture media was collected and Aβ40 was detect by ELISA. The total proteins of U87MG cells from the 2 groups was extractedand BACE1 and PS1 was measure by Western blot.Results: The experimental group and the control group were transfected by plasmids successfully. The expression of EGR1 mRNA increased significantly in the experimental group compared with the control group(P<0.001). Average concentration of Aβ40 was significantly higher in the experimental group than that in the control group(P<0.001). There was a significant increase of BACE1 protein level in the experimental group than that in the control group(P<0.001).There was no significant difference in PS1 protein level between the two groups(P=0.367).Conclusion: EGR1 can increase the expression of Aβ40 in vitro, which may associate with up-regulating the expression of BACE1 protein.

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备注/Memo

备注/Memo:
*河南省科技厅基础与前沿项目 340600531605; 河南省教育厅自然科学研究计划 12A320072
△女,1972年2月生,博士,主任医师,教授,研究方向:神经系统变性病和脑血管病,E-mail:cindy_zhaoxinyu@163.com
更新日期/Last Update: 2016-07-20