[1]刘待见),冯慧芬),刘剑波)#.慢性阻塞性肺疾病模型大鼠骨骼肌中Calpains的表达[J].郑州大学学报(医学版),2016,(04):534-538.
 LIU Daijian),FENG Huifen),LIU Jianbo).Expression of Calpains in skeletal muscle of rat COPD model[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(04):534-538.
点击复制

慢性阻塞性肺疾病模型大鼠骨骼肌中Calpains的表达()
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年04期
页码:
534-538
栏目:
应用研究
出版日期:
2016-07-20

文章信息/Info

Title:
Expression of Calpains in skeletal muscle of rat COPD model
作者:
刘待见1)冯慧芬2)刘剑波1)#
1)郑州大学第二附属医院呼吸内科 郑州 450014;
2)郑州大学第五附属医院感染科 郑州 450052
Author(s):
LIU Daijian1)FENG Huifen2)LIU Jianbo1)
1)Department of Respiratory Diseases,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014;
2)Department of Infectious Diseases,the Fifth Affiliated Hospital,Zhengzhou University, Zhengzhou 450052
关键词:
慢性阻塞性肺疾病 骨骼肌萎缩 Calpain 大鼠
Keywords:
COPD skeletal muscle atrophy Calpain rat
分类号:
R365
摘要:
目的:探讨Calpains在慢性阻塞性肺疾病(COPD)大鼠骨骼肌萎缩中的作用。方法:40只健康雄性Wistar大鼠随机分为COPD模型组及对照组,模型组采用气管内注入脂多糖加香烟烟雾染毒法建立大鼠COPD模型,HE染色法观察肺组织及骨骼肌(趾长伸肌、膈肌)组织的病理学改变; 分别采用免疫组化和RT-PCR法测定骨骼肌组织中Calpains(μ-Calpain和m-Calpain)蛋白及mRNA的表达。结果:在COPD模型组大鼠观察到骨骼肌萎缩,对照组大鼠未见骨骼肌萎缩。与对照组比较,COPD模型组大鼠趾长伸肌及膈肌中μ-Calpain和m-Calpain蛋白及m-Calpain mRNA表达增强(P<0.05),而μ-Calpain mRNA的表达差异无统计学意义(P>0.05)。结论:Calpains在COPD模型大鼠骨骼肌中表达上调,可能参与COPD模型大鼠骨骼肌萎缩。
Abstract:
Aim: To study the role of Calpains in rat skeletal muscle atrophy of chronic obstructive pulmonary disease(COPD).Methods: Forty healthy male Wistar rats were randomly allocated into COPD group and control group. COPD model was established by dripping lipopolysacharide(LPS)into trachea and exposing to cigarettes smoking.The pathological changes of lung tissue and skeletal muscle were observed by HE staining. Immunohistochemistry and RT-PCR were performed respectively to determine protein and mRNA expression of Calpain in skeletal muscle(μ-Calpain and m-Calpain).Results: Skeletal muscle atrophy was observed in COPD group instead of control group.Compared with control group,the expression of μ-Calpain and m-Calpain protein in extensor digitorum longus muscle and diaphragm of COPD group was enhanced(P<0.05), as well as m-Calpain mRNA expression(P<0.05).Conclusion: The expression of Calpains in skeletal muscle of rats COPD model upregulated. Calpains may be involved in COPD skeletal muscle atrophy.

参考文献/References:

[1] 中华医学会呼吸病学分会慢性阻塞性肺疾病学组.2013年慢性阻塞性肺疾病诊治指南(修订版)[J].中华结核和呼吸杂志,2013,36(4):255
[2] 赵磊,姜东林,吕广明,等.单纯运动或感觉神经损伤在骨骼肌萎缩中的致凋亡作用[J].解放军医学杂志,2011,26(9):923
[3] PHELPS KJ,DROUILLARD JS,SILVA MB,et al.Effect of extended postmortem aging and steak location on myofibrillar protein degradation and Warner-Bratzler shear force of beef M. semitendinosus steaks[J].J Anim Sci,2016,94(1):412
[4] MATHUR S,BROOKS D,CARVALHO CR.Structural alterations of skeletal muscle in copd[J].Front Physiol,2014,5:104
[5] YU Y,CHU W,CHAI J,et al.Critical role of miRNAs in mediating skeletal muscle atrophy(Review)[J].Mol Med Rep,2016,13(2):1470
[6] LANGEN RC,GOSKER HR,REMELS AH,et al.The molecular basis of skeletal muscle atrophy[J].Int J Biochem Cell Biol,2013,45(10):2245
[7] KOOHMARAIE M.Ovine skeletal muscle multicatalytic proteinase complex(proteasome): purification, characterization, and comparison of its effects on myofibrils with mu-calpains[J].J Anim Sci,1992,70(12):3697
[8] KENT MP,SPENCER MJ,KOOHMARAIE M.Postmortem proteolysis is reduced in transgenic mice overexpressing calpastatin[J].J Anim Sci,2004,82(3):794
[9] PURINTRAPIBAN J,WANG MC,FORSBERG NE.Degradation of sarcomeric and cytoskeletal proteins in cultured skeletal muscle cells[J].Comp Biochem Physiol B Biochem Mol Biol,2003,136(3):393
[10]BELOZEROVA I,SHENKMAN B,MAZIN M,et al.Effects of long-duration bed rest on structural compartments of m. soleus in man[J].J Gravit Physiol,2001,8(1):P71
[11]FITTS RH,RILEY DR,WIDRICK JJ.Physiology of a microgravity environment invited review:microgravity and skeletal muscle[J].J Appl Physiol(1985),2000,89(2):823
[12]王富霞,夏熙郑,刘待见.慢性阻塞性肺疾病模型大鼠骨骼肌组织中Caspase-12和m-Calpain的表达[J].郑州大学学报(医学版),2014,49(4):508
[13]KRAMEROVA I,KUDRYASHOVA E,VENKATRAMAN G,et al.Calpain 3 participates in sarcomere remodeling by acting upstream of the ubiquitin-proteasome pathway[J].Hum Mol Genet,2005,14(15):2125
[14]TEIXEIRA VDE O,FILIPPIN LI,XAVIER RM.Mechanisms of muscle wasting in sarcopenia[J].Rev Bras Reumatol,2012,52(2):252
[15]DARGELOS E,POUSSARD S,BRULÉ C,et al.Calcium-dependent proteolytic system and muscle dysfunctions: a possible role of calpains in sarcopenia[J].Biochimie,2008,90(2):359

相似文献/References:

[1]冯青青,夏熙郑#.慢性阻塞性肺疾病患者肺组织中Bach1和γGCS的表达[J].郑州大学学报(医学版),2013,(04):474.
 FENG Qingqing,XIA Xizheng.Expressions of Bach1 and γGCS in lung tissue of patients with chronic obstructive pulmonary disease[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(04):474.
[2]王富霞,夏熙郑#,刘待见.慢性阻塞性肺疾病模型大鼠骨骼肌组织中Caspase12和mCalpain的表达[J].郑州大学学报(医学版),2014,(04):508.
 WANG Fuxia,XIA Xizheng,LIU Daijian.Expression of Caspase12 and mCalpain in rat skeletal muscle in the COPD model[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(04):508.
[3]蒋 明,王昌明#,韩旭惠,等.COPD大鼠原代肺动脉平滑肌细胞中MCP-1、TGF-β1和TLR4蛋白的检测*[J].郑州大学学报(医学版),2017,(05):641.[doi:10.13705/j.issn.1671-6825.2017.05.031]
 JIANG Ming,WANG Changming,HAN Xuhui,et al.Detection of MCP-1,TGF-β1 and TLR4 protein in primary pulmonary artery smooth muscle cells of COPD rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(04):641.[doi:10.13705/j.issn.1671-6825.2017.05.031]

备注/Memo

备注/Memo:
#通信作者,男,1964年2月生,博士,教授,主任医师,研究方向:慢性气道炎症性疾病,E-mail:jbliuzz@126.com
更新日期/Last Update: 2016-07-20