[1]孙 艳),张 华),封青川),等.Spink8基因对EC9706细胞增殖、凋亡及迁移能力的影响*[J].郑州大学学报(医学版),2016,(05):568-571.
 SUN Yan),ZHANG Hua),FENG Qingchuan),et al.Effects of Spink8 gene on proliferation,apoptosis and migration of EC9706 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(05):568-571.
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Spink8基因对EC9706细胞增殖、凋亡及迁移能力的影响*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年05期
页码:
568-571
栏目:
食管癌研究
出版日期:
2016-08-20

文章信息/Info

Title:
Effects of Spink8 gene on proliferation,apoptosis and migration of EC9706 cells
作者:
孙 艳12)张 华3)封青川1)范玉佳1)李 涛1)张玉超1)贺 颖1)郑 红1)#
1)郑州大学基础医学院医学遗传学与细胞生物学系 郑州 450001;2)河南省中医药大学第一附属医院生殖医学科 郑州 450003;3)郑州市妇幼保健院优生科 郑州 450012
Author(s):
SUN Yan12) ZHANG Hua3) FENG Qingchuan1) FAN Yujia1) LI Tao1) ZHANG Yuchao1) HE Ying1) ZHENG Hong1)
1)Department of Medical Genetics and Cell Biology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001;2)Department of Reproductive Medicine,the First Affiliated Hospital,Henan University of Traditional Chinese Medicine,Zhengzhou 450003;3)Prepotency Division,Women and Infants Hospital of Zhengzhou,Zhengzhou 450012
关键词:
Spink8基因 食管癌细胞 增殖 凋亡 细胞迁移
Keywords:
Spink8 gene esophageal cancer cell proliferation apoptosis cell migration
分类号:
R735.1
摘要:
目的:探讨Spink8基因对人食管癌EC9706细胞增殖、凋亡及迁移能力的影响。方法:利用DNA重组技术构建Spink8真核表达重组质粒并转染EC9706细胞,构建稳定表达Spink8的EC9706细胞。取未转染、转染空质粒和稳定表达Spink8的EC9706细胞,采用RT-PCR和Western blot 法检测Spink8 mRNA和蛋白,MTT法检测细胞增殖,Annexin V-APC/7-AAD法检测细胞凋亡,并进行Transwell细胞迁移实验。结果:与未转染和转染空质粒组细胞比较,稳定表达Spink8的EC9706细胞增殖受到抑制(P<0.05),增殖抑制率为24.5%; 细胞凋亡率增加(P<0.05); Transwell小室穿膜细胞数减少(P<0.05)。结论:Spink8可能是一个新的抑癌基因。
Abstract:
Aim: To investigate the effects of Spink8 gene on proliferation, apoptosis and migration of human esophageal cancer cell EC9706.Methods: Eukaryotic expression plasmid pEGFP-Spink8 was established by DNA recombination in vitro, and transfected into EC9706 cells. The expressions of Spink8 mRNA and protein were examined by RT-PCR and Western blot. MTT method was used to detect cell proliferation, Annexin V-APC/7-AAD method was used to detect cell apoptosis, and migration ability was detected by Transwell assay.Results: The plasmid of pEGFP-Spink8 was successfully established. Compared with the EC9706 cells without transfection or transfected with null plasmid, the number of surviving cells transfected with pEGFP-Spink8 decreased(P<0.05), and proliferation inhibition rate was 24.5%; the apoptosis rate was significantly increased(P<0.05); the number of cells which passed Transwell chamber decreased(P<0.05).Conclusion: Spink8 may be a new tumor suppressor gene.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省医学科技攻关计划 201303001
#通信作者,女,1966年3月生,博士,教授,研究方向:遗传性疾病的致病基因的筛选与研究,E-mail:hzheng@zzu.edu.cn
更新日期/Last Update: 2016-08-20