[1]李银英),陈成群),张振中)#.2-甲氧基雌二醇联合冬凌草甲素对胃癌SGC-7901细胞增殖和凋亡的影响*[J].郑州大学学报(医学版),2016,(05):602-606.
 LI Yinying),CHEN Chengqun),ZHANG Zhenzhong).Effect of 2-methoxy estradiol and oridonin on proliferation and apoptosis of gastric cancer SGC-7901 cells in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(05):602-606.
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2-甲氧基雌二醇联合冬凌草甲素对胃癌SGC-7901细胞增殖和凋亡的影响*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年05期
页码:
602-606
栏目:
论著
出版日期:
2016-08-20

文章信息/Info

Title:
Effect of 2-methoxy estradiol and oridonin on proliferation and apoptosis of gastric cancer SGC-7901 cells in vitro
作者:
李银英1)陈成群2)张振中3)#
1)郑州大学第二附属医院药学部 郑州 450014;2)郑州大学第一附属医院药学部 郑州 450052;3)郑州大学药学院 郑州450001
Author(s):
LI Yinying1)CHEN Chengqun2)ZHANG Zhenzhong3)
1)Department of Pharmacology,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014;2)Department of Pharmacology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052;3)College of Pharmacology,Zhengzhou University,Zhengzhou 450001
关键词:
2-甲氧基雌二醇 增殖 凋亡 胃癌 联合用药 SGC-7901细胞
Keywords:
2-methoxy estradiol proliferation apoptosis gastric cancer SGC-7901 cell
分类号:
R735
摘要:
目的:研究2-甲氧基雌二醇(2-ME)单独和联合冬凌草甲素对胃癌SGC-7901细胞增殖和凋亡的影响。方法:用0.125、0.250、0.500、1.000、2.000、4.000、8.000、16.000 μmol/L 2-ME处理SGC-7901细胞24、48和72 h后,观察细胞形态变化,SRB法检测2-ME对SGC-7901细胞增殖的抑制作用; 用0.625、2.500、10.000、20.000 μmol/L 2-ME以及相同浓度的冬凌草甲素单独或联合应用处理SGC-7901细胞48 h后,计算结合指数,用流式细胞术和荧光染色法观察细胞凋亡情况。结果:2-ME作用于SGC-7901细胞48 h的IC50值为5.31 μmol/L,对细胞SGC-7901的抑制作用呈时间依赖性,与冬凌草甲素联合应用的IC50值为8.46 μmol/L。不同浓度2-ME作用48 h,随着剂量增加,SGC-7901细胞凋亡率增加,联合应用冬凌草甲素对细胞凋亡的影响未表现出协同作用。结论:2-ME可以抑制SGC-7901细胞的增殖,诱导其凋亡,与冬凌草甲素联合应用时对肿瘤细胞增殖的抑制表现为相加作用,对凋亡未表现出协同作用。
Abstract:
Aim: To study the effect of 2-methoxy estradiol(2-ME)separate and combined with oridonin treatment on proliferation and apoptosis of gastric cancer strain SGC-7901 in vitro.Methods: SGC-7901 cells were treated with 0.125,0.250,0.500,1.000,2.000,4.000,8.000,16.000 μmol/L 2-ME for 24, 48 and 72 h, cells morphological changes were observed,and SRB assay was applied to detect the SGC-7901 cell growth; with different concentration(0.625,2.500,10.000,20.000 μmol/L)2-ME and the same concentration oridonin separately treatment on SGC-7901 cells for 48 h, the combination index was calculated,and the apoptosis of the cells by flow cytometry and fluorescent staining was observed.Results: The IC50 of 2-ME acted on SGC-7901 cells was 5.31 μmol/L, and the inhibitory effect of 2-ME on SGC-7901 cells was concentration- and time- dependent. After being treated by different concentrations of 2-ME for 48 h, the apoptosis rate of SGC-7901 cells was increased with the increase of the dose, and the effect of 2-ME on apoptosis had no synergistic.Conclusion: 2-ME can inhibit the proliferation of SGC-7901 cells, induce apoptosis, and enhance the effect of oridonin.

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目 81573364
#通信作者,男,1964年11月生,博士,教授,研究方向:肿瘤靶向治疗,E-mail:zhangzz08@126.com
更新日期/Last Update: 2016-08-20