[1]杨再刚△,陈玉娟,赵 倩,等.不同胰岛素水平和脂类饮食对小鼠肥胖和脂肪细胞的影响*[J].郑州大学学报(医学版),2016,(05):627-630.
 YANG Zaigang,CHEN Yujuan,ZHAO Qian,et al.Effects of different insulin levels and lipid diet on obesity and fat cells in mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(05):627-630.
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不同胰岛素水平和脂类饮食对小鼠肥胖和脂肪细胞的影响* ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年05期
页码:
627-630
栏目:
应用研究
出版日期:
2016-08-20

文章信息/Info

Title:
Effects of different insulin levels and lipid diet on obesity and fat cells in mice
作者:
杨再刚陈玉娟赵 倩母金娣陈 旭
郑州大学第一附属医院老年内分泌科 郑州 450052
Author(s):
YANG ZaigangCHEN YujuanZHAO QianMU JindiCHEN Xu
Department of Geriatric Endocrinology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
胰岛素 肥胖 过氧化物酶增殖物活化受体γ2 小鼠
Keywords:
insulin obesity peroxisome proliferator-activated receptor-γ2 mouse
分类号:
R589.9
摘要:
目的:研究不同胰岛素水平和脂类饮食对小鼠肥胖的影响。方法:60只小鼠根据空腹胰岛素水平及饮食状况分为高胰岛素高脂饮食组(HH组)、高胰岛素普通饮食组(HN组)、低胰岛素高脂饮食组(LH组)、低胰岛素普通饮食组(LN组),每组15只。12周后称量小鼠体重并计算体重增加量,分离肾周及肠系膜周围脂肪组织并称量,荧光定量PCR及Western blot法测定各组脂肪组织过氧化物酶增殖物活化受体γ2(PPARγ2)mRNA及蛋白的表达水平。结果:12周末,HH组体重增加量、脂肪组织质量、PPARγ2 mRNA及蛋白表达水平均高于其他3组(P<0.05)。结论:高胰岛素水平促进高脂饮食介导的肥胖,其机制可能与PPARγ2表达有关。
Abstract:
Aim: To investigate the effects of different insulin levels and lipid diet on obesity and fat cells in mice.Methods: According to the fasting insulin level and diet,60 mice were allocated into high insulin level-high fat diet group(HH group,n=15), high insulin level-normal fat diet group(HN group,n=15), low insulin level-high fat diet group(LH group,n=15),and low insulin level-normal fat diet group(LN group,n=15). After 12 weeks, the mice weight and the adipose tissue around the kidney and the mesentery were weighed, the mRNA and protein expressions of peroxisome proliferator-activated receptor-γ2(PPARγ2)were detected by fluorescent quantitative PCR and Western blot, respectively.Results: After 12 weeks, the growth of mice weight and adipose tissue weight and the mRNA and protein expressions of PPARγ2 of HH group were significantly higher than those of the other 3 groups(P<0.05).Conclusion: High insulin level could promote obesity which caused by high fat diet,and its mechanism may be related to the expression of PPARγ2.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省医学科技攻关计划项目 201403043
△男,1971年10月生,博士,副主任医师,研究方向:代谢疾病,E-mail:446957419@qq.com
更新日期/Last Update: 2016-08-20