[1]弓园芳),施梦丽),张 勤),等.血管性痴呆患者MTHFR基因多态性和血浆Hcy水平检测*[J].郑州大学学报(医学版),2016,(05):647-650.
 GONG Yuanfang),SHI Mengli),ZHANG Qin),et al.Detection of MTHFR gene polymorphism and plasma homocysteine levels in patients with vascular dementia[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(05):647-650.
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血管性痴呆患者MTHFR基因多态性和血浆Hcy水平检测*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年05期
页码:
647-650
栏目:
应用研究
出版日期:
2016-08-20

文章信息/Info

Title:
Detection of MTHFR gene polymorphism and plasma homocysteine levels in patients with vascular dementia
作者:
弓园芳1)施梦丽2)张 勤1)崔 凯1)陈淅泠1)#
1)郑州大学第二附属医院老年医学科 郑州 450014;2)郑州大学第二附属医院核医学科 郑州 450014
Author(s):
GONG Yuanfang1)SHI Mengli2)ZHANG Qin1)CUI Kai1)CHEN Xiling1)
1)Department of Geriatrics,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014; 2)Department of Nuclear Medicine,the Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450014
关键词:
血管性痴呆 基因多态性 同型半胱氨酸 叶酸 维生素B12
Keywords:
vascular dementia gene polymorphism homocysteine folate Vitamin B12
分类号:
R743
摘要:
目的:探讨血管性痴呆(VaD)与MTHFR基因多态性和血浆同型半胱氨酸(Hcy)水平的关系。方法:选取73例VaD患者和75例非痴呆脑梗死患者,使用简易精神状态检查量表(MMSE)评定VaD患者痴呆严重程度。应用PCR-芯片杂交法测定其MTHFR基因多态性,酶循环法测定血浆Hcy水平,免疫分析法测定叶酸、维生素B12水平。结果:VaD组MTHFR基因TT基因型及T等位基因频率高于非痴呆脑梗死组(P<0.05),MTHFR基因TT、CT型血浆Hcy水平高于CC型(P<0.001); VaD组血浆Hcy水平显著高于非痴呆脑梗死组(P<0.001),痴呆程度与血浆Hcy水平呈正相关(r=0.227,P=0.018); 非痴呆脑梗死组叶酸、维生素B12水平高于VaD组(P<0.05)。结论:MTHFR基因C677T位点突变及高Hcy血症可能和VaD的发生相关。
Abstract:
Aim: To investigate the association between MTHFR gene polymorphism and plasma homocysteine(Hcy)levels in patients with vascular dementia(VaD).Methods: A total of 73 patients with VaD and 75 patients with nondemented cerebral infarction were recruited in the study. PCR-RFLP was used to analyze the polymorphism of MTHFR gene. The folic acid and vitamin B12 levels were measured using immunoassay method. And circulating enzymatic method was empolyed to test the Hcy concentration in plasma. The degree of dementia was classified according to the MMSE for patients with VaD.Results: The genotype TT and T allele frequency of MTHFR gene in VaD group were significantly higher than that in nondementia cerebral infarction group(P<0.05). The concentration of plasma Hcy was higher in patients with TT and CT type than those with CC genotype(P<0.001). Mean total plasma Hcy concentration was significantly higher in VaD patients than that in the patients with nondemented cerebral infarction(P<0.001), but the levels of folate and vitamin B12 were significantly lower in VaD patients than in the patients with nondemented cerebral infarction(P<0.05). The degree of dementia was positively related with plasma Hcy level(r=0.227,P=0.018).Conclusion: Genetic mutations of MTHFR C677T and the hyperhomocysteinemia may closely related to the occurrence of VaD.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省医学科技攻关计划项目 201403095
#通信作者,女,1964年4月生,硕士,主任医师,研究方向:老年痴呆,E-mail:13938289530@126.com
更新日期/Last Update: 2016-08-20