[1]蒋清虎)△,马吉安),谭孝华),等.siRNA沉默ARK5基因对SMMC-7721细胞侵袭及MMP-2蛋白分泌的影响[J].郑州大学学报(医学版),2016,(06):723-726.
 JIANG Qinghu),MA Ji'an),TAN Xiaohua),et al.Effect of siRNA silencing ARK5 gene on invasion and secretion of MMP-2 of SMMC-7721 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(06):723-726.
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siRNA沉默ARK5基因对SMMC-7721细胞侵袭及MMP-2蛋白分泌的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年06期
页码:
723-726
栏目:
论著
出版日期:
2016-11-20

文章信息/Info

Title:
Effect of siRNA silencing ARK5 gene on invasion and secretion of MMP-2 of SMMC-7721 cells
作者:
蒋清虎1)△马吉安1)谭孝华1)吴忠均2)
1)达州市中西医结合医院肝胆外科 达州 635000;
2)重庆医科大学附属第一医院肝胆外科 重庆 400016
Author(s):
JIANG Qinghu1)MA Ji'an1) TAN Xiaohua1) WU Zhongjun2)
1)Department of Hepatobiliary Surgery,Dazhou Chinese Integrative Medicine Hospital,Dazhou 635000;
2)Department of Hepatobiliary Surgery,the First Affiliated Hospital,Chongqing Medical University,Chongqing 400016
关键词:
肝癌 侵袭 ARK5基因 RNA干扰 SMMC-7721细胞
Keywords:
hepatocellular carcinoma invasion ARK5 RNA interference SMMC-7721 cell
分类号:
R735.7
摘要:
目的:观察siRNA沉默ARK5基因的表达对肝癌SMMC-7721细胞侵袭及MMP-2蛋白分泌的影响。方法:采用qRT-PCR和Western blot法检测SMMC-7721细胞和正常肝细胞LO2中ARK5的表达。体外合成靶向ARK5基因的siRNA,转染肝癌SMMC-7721细胞,并设阴性对照和空白对照,采用Western blot方法检测3组细胞中ARK5蛋白的表达,通过Transwell 小室模型检测细胞的体外侵袭能力,采用ELISA法检测细胞培养基中MMP-2蛋白的含量。结果:SMMC-7721细胞中ARK5 mRNA和蛋白的表达水平高于LO2细胞(P<0.05); 沉默ARK5基因后,SMMC-7721细胞的侵袭受到抑制,且MMP-2蛋白的分泌水平低于阴性对照和空白对照(P<0.05)。结论:沉默ARK5基因可能通过MMP-2途径抑制SMMC-7721细胞的侵袭。
Abstract:
Aim: To study the effect of ARK5 gene silencing on the invasion of SMMC-7721 cells.Methods: The expression of ARK5 was determined by qRT-PCR and Western blot in SMMC-7721 and LO2 cells,respectively. ARK5 siRNA was constructed and transfected into SMMC-7721 cells, and negative group and blank group were set up; the expression of ARK5 protein was determined by Western blot,and the invasion of cells in 3 groups were examined by Transwell assay. MMP-2 protein in culture medium was determined by ELISA.Results: The mRNA and protein expression levels of ARK5 in SMMC-7721 cells were significantly higher than those in LO2 cells(P<0.05). After ARK5 siRNA transfection, the invasion of SMMC-7721 cells was significantly inhibited,and MMP-2 protein secreted by the cells was significantly lower than those in negative group and blank group(P<0.05).Conclusion: ARK5 silencing could effectively inhibit invasion of SMMC-7721 cells via MMP-2 pathway.

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备注/Memo

备注/Memo:
△男,1987年8月生,硕士,医师,研究方向:肝癌及其机制,E-mail:qinghucqmu@163.com
更新日期/Last Update: 2016-11-20