[1]崔瑞新,吕风华#,岳 莹,等.microRNA-499靶控的PI3K/Akt通路在大鼠缺血-再灌注心肌损伤中的作用*[J].郑州大学学报(医学版),2016,(06):740-743.
 CUI Ruixin,LYU Fenghua,YUE Ying,et al.Effect of microRNA-499 targeted at PI3K/Akt signaling pathway on myocardial ischemia-reperfusion in rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(06):740-743.
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microRNA-499靶控的PI3K/Akt通路在大鼠缺血-再灌注心肌损伤中的作用*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年06期
页码:
740-743
栏目:
论著
出版日期:
2016-11-20

文章信息/Info

Title:
Effect of microRNA-499 targeted at PI3K/Akt signaling pathway on myocardial ischemia-reperfusion in rats
作者:
崔瑞新吕风华#岳 莹刘亚坤高建芝
新乡医学院第一附属医院心内一科 新乡 453100
Author(s):
CUI RuixinLYU FenghuaYUE YingLIU YakunGAO Jianzhi
First Department of Cardiovascular Medicine,the First Clinical College,Xinxiang Medical University,Xinxiang 453100
关键词:
心肌 缺血-再灌注损伤 microRNA-499 PI3K Akt 大鼠
Keywords:
myocardium ischemia-reperfusion injury microRNA-499 PI3K Akt rat
分类号:
R542.2
摘要:
目的:探讨microRNA-499在大鼠心肌缺血-再灌注损伤(MIRI)中的作用及其与PI3K/Akt通路的关系。方法:将30只SD大鼠随机分为假手术组、模型组和干预组,每组10只。假手术组仅在冠状动脉的左前降支下穿线但并不结扎,模型组结扎冠状动脉左前降支以建立大鼠MIRI模型(缺血30 min,再灌注120 min),干预组于建立MIRI模型前48 h注射microRNA-499激动剂。采用荧光定量RT-PCR法检测大鼠心肌组织中miRNA-499及PI3K mRNA表达量,Western blot法检测Akt磷酸化活性,采用TTC染色法检测心肌梗死面积。结果:与假手术组相比,模型组miRNA-499及PI3K mRNA表达量减少,Akt磷酸化活性明显降低,心肌梗死面积明显增加(P<0.05)。与模型组比较,干预组miRNA-499及PI3K mRNA表达量、Akt磷酸化活性明显升高,心肌梗死面积明显减少(P<0.05)。心肌组织中PI3K mRNA与microRNA-499的表达量呈正相关(P<0.05)。结论:microRNA-499可能通过激活PI3K/Akt信号通路,减轻大鼠MIRI。
Abstract:
Aim: To investigate the effect of miRNA-499 on myocardial ischemia-reperfusion injury(MIRI)of rats and its relation with PI3K/Akt signaling pathway.Methods: Thirty SD rats were randomly assigned into sham-operation group, model group, and precondition group with 10 rats in each group. In the sham-operation group, the thread was only threaded under left anterior descending coronary artery(LAD)but not ligated; in model group, MIRI models were built by ligating LAD with 30 min ischemia and 120 min reperfusion; in the precondition group, rats were injected miRNA-499 agomir 48 h before MIRI operation. Real Time RT-PCR was adopted to detect the expressions of miRNA-499 and PI3K mRNA, Western blot was adopted to detect Akt phosphorylation level, and the myocardial infarction size were measured using TTC staining.Results: Compared with sham-operation group, the expressions of miRNA-499 and PI3K mRNA, and the Akt phosphorylation level in model group were significantly lower,and the infarction size were larger(P<0.05). Compared with those in MIRI group, the expressions of miRNA-499 and PI3K mRNA, and the Akt phosphorylation level in precondition group were obviously higher,while the infarction size was smaller(P<0.05). The expression of microRNA-499 was positively related to that of PI3K mRNA(P<0.05)。Conclusion: microRNA-499 could reduce MIRI via activating the PI3K/Akt signaling pathway.

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备注/Memo

备注/Memo:
*河南省高等学校青年骨干教师资助计划 2009GGJS-085
#通信作者,女,1970年10月生,博士,教授,主任医师,研究方向:冠心病的基础与临床研究, E-mail:doctorlvfh@163.com
更新日期/Last Update: 2016-11-20