[1]苗金红),朱海洋),钟 斌),等.褪黑素对急性脊髓损伤大鼠脊髓组织中MPO及CINC-1表达的影响*[J].郑州大学学报(医学版),2016,(06):757-761.
 MIAO Jinhong),ZHU Haiyang),ZHONG Bin),et al.Effects of melatonin on expression of MPO and CINC-1 in rats with acute spinal cord injury[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(06):757-761.
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褪黑素对急性脊髓损伤大鼠脊髓组织中MPO及CINC-1表达的影响*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年06期
页码:
757-761
栏目:
应用研究
出版日期:
2016-11-20

文章信息/Info

Title:
Effects of melatonin on expression of MPO and CINC-1 in rats with acute spinal cord injury
作者:
苗金红1)朱海洋2)钟 斌2)崔 浩2)汪 鑫2)理 阳2)徐玉生2)#
1)郑州大学第一附属医院 郑州 450052;
2)郑州大学第一附属医院骨科 郑州 450052
Author(s):
MIAO Jinhong1) ZHU Haiyang2) ZHONG Bin2) CUI Hao2) WANG Xin2)LI Yang2) XU Yusheng2)
1)The First Affiliated Hospital,Zhengzhou 450052;
2)Department of Orthopedics,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
褪黑素:脊髓损伤:中性粒细胞趋化因子 髓过氧化物酶 大鼠
Keywords:
melatonin spinal cord injury cytokine-induced neutrophil chemoattractant myeloperoxidase rat
分类号:
R681.5+4
摘要:
目的:探讨褪黑素(MT)对大鼠急性脊髓损伤后髓过氧化物酶(MPO)及中性粒细胞趋化因子(CINC)-1表达的影响。方法:108只SD大鼠分为假手术组、脊髓损伤组、褪黑素治疗组。手术组大鼠仅切除T11椎板,脊髓损伤组、褪黑素治疗组建立T11脊髓损伤模型。脊髓损伤组、褪黑素治疗组在脊髓损伤后10 min内分别腹腔注射含体积分数5%乙醇的生理盐水和100 mg/kg的褪黑素制剂。于脊髓损伤后1 h、6 h、12 h、1 d、3 d、5 d分别取脊髓组织,化学法测定MPO活力变化,HE染色法观察脊髓组织结构,RT-PCR法观察CINC-1 mRNA表达情况。结果:脊髓内CINC-1水平和MPO活性有相似的变化规律, 且MPO在CINC-1表达达峰之后12 h达高峰; 除1 h外各时间点各组相比较,B组最高、C组次之、A组最低,其中B组与A组、C组差异具有统计学意义(P<0.05)。结论:褪黑素可通过抑制CINC-1的表达减少中性粒细胞的浸润,从而减轻炎症反应,起到一定的神经保护作用。
Abstract:
Aim: To investigate the effects of melatonin(MT)on expression of myeloperoxidase(MPO)and cytokine-induced neutrophil chemoattractant(CINC)-1 in rats after acute spinal cord injury.Methods: Totally 108 SD rats were randomly allocated into 3 groups:sham operation group(group A,n=36),spinal cord injury group(group B,n=36), and MT treatment group(group C,n=36). Group B and C were established SCI animal models at T11 level. Lamina of vertebra was cut without spinal cord injury in the sham operation group. The rats of group C were injected 5%(V/V)alcohol into abdominal cavity after spinal cord injury while the rats of group B were injected MT(100 mg/kg). Fetching the spinal tissue at 1 h, 6 h, 12 h, 1 d, 3 d and 5 d after spinal cord injury. Chemical method was used to detect the expression of MPO. HE staining was performed to observe the pathological changes of the spinal cord in the 3 groups respectively. RT-PCRwas used to detect the expression of CINC-1 mRNA in spinal cord tissue.Results: There were similar changing patterns of contents of CINC-1 and MPO, and the peak time of MPO was 12 h later than CINC-1. Compared the three groups except 1 h,group B had the highest level and group A had the lowest level regarding to the contents of CINC-1 and MPO, and the differences among them were statistical(P<0.05).Conclusion: MT could inhibit the expression of CINC-1 and reduce neutrophil infiltration,thus relieve inflammation reaction and protect spinal cord from damage.

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备注/Memo

备注/Memo:
*河南省国际科技合作项目 134300510005
#通信作者,男,1969 年 3 月生,博士,主任医师,研究方向:骨科疾病的基础与临床,E-mail:ysxu@zzu.edu.cn
更新日期/Last Update: 2016-11-20