[1]陈 伟,马 磊,杨立山#.甘草次酸对哮喘大鼠的抗氧化应激作用及NF-κB信号通路的调控*[J].郑州大学学报(医学版),2016,(06):762-765.
 CHEN Wei,MA Lei,YANG Lishan.Effects of glycyrrhetinic acid on oxidative stress and NF-κB signal pathway in bronchial asthma rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2016,(06):762-765.
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甘草次酸对哮喘大鼠的抗氧化应激作用及NF-κB信号通路的调控*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2016年06期
页码:
762-765
栏目:
应用研究
出版日期:
2016-11-20

文章信息/Info

Title:
Effects of glycyrrhetinic acid on oxidative stress and NF-κB signal pathway in bronchial asthma rats
作者:
陈 伟马 磊杨立山#
宁夏医科大学总医院急诊科 银川 750004
Author(s):
CHEN Wei MA Lei YANG Lishan
Department of Emergency, General Hospital of Ningxia Medical University, Yinchuan 750004
关键词:
甘草次酸 支气管哮喘 NF-κB 氧化应激 大鼠
Keywords:
glycyrrhetinic acid bronchial asthma NF-κB oxidative stress rat
分类号:
R285.5
摘要:
目的:探讨甘草次酸对哮喘大鼠肺组织NF-κB、IL-6、IL-5蛋白表达及抗氧化应激能力的影响。方法:采用卵清白蛋白致敏法制备大鼠支气管哮喘模型,并给予甘草次酸高、中、低(200、100、50 mg/kg)剂量进行干预; 同时设正常对照和地塞米松对照; 每组10只大鼠。干预7 d后处死大鼠,用生化法测大鼠血清超氧化物歧化酶(SOD)、丙二醛(MDA)、总抗氧化能力(T-AOC)及谷胱甘肽过氧化物酶(GSH-Px),用RT-PCR和Western-blot法测大鼠肺组织NF-κB、IL-6及IL-5 mRNA及蛋白表达水平。结果:与正常对照比较,模型组和GA各剂量组大鼠血清SOD、GSH-Px及T-AOC水平降低,MDA水平升高,肺组织中NF-κB、IL-6及IL-5 mRNA和蛋白表达水平升高(P<0.05)。与模型组比较,甘草次酸高、中剂量组大鼠血清SOD、GSH-Px水平升高, MDA水平降低(P<0.05),肺组织中NF-κB、IL-6及IL-5 mRNA和蛋白表达水平降低(P<0.05),其中甘草次酸高剂量组变化更显著。结论:甘草次酸对哮喘大鼠氧化应激有拮抗作用,其机制与调控NF-κB信号通路有关。
Abstract:
Aim: To investigate the effects of glycyrrhetinic acid(GA)on the NF-κB signal pathway and anti-oxidative stress capability in experimental asthma rats.Methods: Ovalbumin allergization was used to establish bronchial asthma rat model, and the model rats were given GA(200, 100, 50 mg/kg)through intragastric administration once a day for 7 days, respectively,blank control, asthma model control and dexamethasone control were employed in the experiment,and 10 rats were in each group. The levels of SOD, MDA, GSH-Px and T-AOC in serum were determined by biochemical methods. The mRNA and protein expressions of NF-κB, IL-6 and IL-5 in lung tissue were determined by RT-PCR and Western blot,respectively.Results: Compared with the blank control group, the activity of SOD, GSH-Px and T-AOC in serum of asthma model control group and 3 GA groups decreased, the serum level of MDA was higher, and the mRNA and protein expressions of NF-κB, IL-6 and IL-5 in lung tissue were higher(P<0.05). Compared with the asthma model control group, the activity of SOD and GSH-Px in serum of 200 and 100 mg/kg GA group were higher, the serum level of MDA was lower, the mRNA and protein expressions of NF-κB, IL-6 and IL-5 in lung tissue were lower(P<0.05),and the changes of 200 mg/kg GA group were more obvious.Conclusion: GA has the capability to against oxidative stress in asthma rats, which may be related to the NF-κ B signal pathway.

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备注/Memo

备注/Memo:
*宁夏科技支撑计划项目(2015)
#通信作者,男,1965年4月生,硕士,教授,主任医师,研究方向:临床药理学,E-mail:nyfyyls@126.com
更新日期/Last Update: 2016-11-20