[1]方 莉),王赛琪),陈雪梅),等.冬凌草活性成分KY3对食管癌EC109 细胞增殖的抑制作用*[J].郑州大学学报(医学版),2017,(01):1-4.[doi:10.13705/j.issn.1671-6825.2017.01.001]
 FANG Li),WANG Saiqi),CHEN Xuemei),et al.Inhibitory effect of active ingredient of Rabdosia rubescens KY3 on proliferation of esophageal carcinoma EC109 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(01):1-4.[doi:10.13705/j.issn.1671-6825.2017.01.001]
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冬凌草活性成分KY3对食管癌EC109 细胞增殖的抑制作用*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年01期
页码:
1-4
栏目:
食管癌研究
出版日期:
2017-01-15

文章信息/Info

Title:
Inhibitory effect of active ingredient of Rabdosia rubescens KY3 on proliferation of esophageal carcinoma EC109 cells
作者:
方 莉1)王赛琪2)陈雪梅3)薛博涵3)王冠涛3)李玉萍3)刘宏民2)#
1)郑州大学医院检验科 郑州 450001;
2)郑州大学药学院 郑州 450001;
3)郑州大学基础医学院人体解剖学系 郑州 450001
Author(s):
FANG Li1) WANG Saiqi2) CHEN Xuemei3) XUE Bohan3) WANG Guantao3) LI Yuping3) LIU Hongmin2)
1)Clinical Laboratory,Hospital of Zhengzhou University, Zhengzhou 450001;
2)School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001;
3)Department of Human Anatomy, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001
关键词:
EC109细胞 凋亡 周期阻滞 KY3
Keywords:
EC109 cell apoptosis cycle arrest KY3
分类号:
R979.1
DOI:
10.13705/j.issn.1671-6825.2017.01.001
摘要:
目的:探讨冬凌草活性成分KY3对食管癌细胞EC109增殖的抑制作用及其机制。方法:MTT法测定0、10、20、30、40、60、80、100 μmol/L KY3对EC109细胞增殖的影响; 流式细胞术检测0、10、20、30 μmol/L KY3对EC109细胞凋亡和细胞周期分布的影响,免疫荧光法观察KY3对EC109细胞中微管结构的影响,Hoechst 33258染色观察KY3作用后EC109细胞核的形态。结果:KY3能够抑制EC109细胞的体外增殖; KY3处理后EC109细胞周期阻滞在G2/M期并发生凋亡(P<0.05); KY3作用于EC109细胞后,微管遭到破坏,随着KY3剂量的增加,α-微管蛋白逐渐发生降解并出现细胞核固缩、浓染等典型的凋亡形态。结论:冬凌草活性成分KY3在体外对EC109细胞具有增殖抑制作用,其机制可能与破坏细胞微管结构、G2/M期细胞周期阻滞及诱导细胞凋亡有关。
Abstract:
Aim: To investigate the proliferation inhibition and the mechanism of active ingredients of Rabdosia rubescens KY3 on esophageal carcinoma EC109 cells.Methods: MTT assay was performed to measure the effect of 0,10,20,30,40,60,80,100 μmol/L KY3 on EC109 cell proliferation. Flow cytometry assay was conducted to measure the effect of 0,10,20,30 μmol/L KY3 on apoptosis and cell cycle distribution of EC109 cells, immunofluorescence was used to determine the effect of KY3 on microtubule structure of EC109 cells, and Hoechst 33258 staining was used to detect the morphological changes of the nucleus of EC109 cells after the treatment of KY3.Results: KY3 could inhibit the proliferation of EC109 cells in vitro,and induce cell cycle arrest in G2/M phase as well as apoptosis of EC109 cells(P<0.05). After being treated by KY3, the microtubule of EC109 cells was destroyed. After the dose of KY3 increasing, α-microtubulin degradated gradually and had a typical morphological characteristic of apoptosis such as nucleus pycnosis and bright nuclei.Conclusion: The active ingredient of Rabdosia rubescens KY3 could inhibit the proliferation of EC109 cells in vitro, and the underlying mechanisms may be related to the destruction of microtubule, the cell cycle arrest at G2/M phase as well as the induction of the apoptosis of EC109 cells.

参考文献/References:

[1] OKA M,YAMAMOTO K,TAKAHASHI M,et al.Relationship between serum levels of interleukin 6, various disease parameters and malnutrition in patients with esophageal squamous cell carcinoma[J].Cancer Res,1996,56(12):2776
[2] 刘净,梁敬钰,谢韬.冬凌草研究进展[J].海峡药学,2004,16(2):1
[3] LIU Z,OUYANG L,PENG H,et al.Oridonin: targeting programmed cell death pathways as an anti-tumour agent[J].Cell Prolif,2012,45(6):499
[4] LI CY,WANG EQ,CHENG Y,et al.Oridonin:an active diterpenoid targeting cell cycle arrest, apoptotic and autophagic pathways for cancer therapeutics[J]. Int J Biochem Cell Biol,2011,43(5):701
[5] CHEN SS,MICHAEL A,BUTLER-MANUEL SA.Advances in the treatment of ovarian cancer: a potential role of antiinflammatory phytochemicals[J].Discov Med,2012,13(68):7
[6] 崔侨,田代真一,小野寺敏,等.冬凌草甲素通过诱导人宫颈癌HeLa细胞自噬下调凋亡的机制[J].药学学报,2007,42(1):35
[7] 潘剑,李绍平,李建雄,等.羟基红花黄色素A对MPP+诱导的SH-SY5Y细胞凋亡的保护作用[J].西安交通大学学报(医学版),2014,35(5):690
[8] 王光兰,崔俊生,王心蕊,等.20(S)-人参皂苷Rg3诱导肺癌细胞NCI-H460凋亡的机制[J].吉林大学学报(医学版),2008,34(6):980
[9] 刘亮,左连富,王静.青蒿琥酯对食管癌Ec9706细胞线粒体膜电位及凋亡的影响[J].解放军医学杂志,2014,39(1):25
[10]李银英,陈成群,张振中.2-甲氧基雌二醇联合冬凌草甲素对胃癌 SGC-7901细胞增殖和凋亡的影响[J].郑州大学学报(医学版),2016,51(5):602
[11]DUMONTET C, JORDAN MA. Microtubule-binding agents: a dynamic field of cancer therapeutics[J]. Nat Rev Drug Discov,2010,9(10):790
[12]尚海,潘莉,杨澍,等.微管蛋白抑制剂的研究进展[J].药学学报,2010,45(9):1078
[13]PASQUIER E,KAVALLARIS M.Microtubules: a dynamic target in cancer therapy[J].IUBMB Life,2008,60(3):165
[14]徐铮奎.冬凌草有望成为“紫杉醇第二”[J].中国现代中药,2007,9(8):43

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备注/Memo

备注/Memo:
*国家自然科学基金面上项目 81430085,21372206,81172937; 河南省科技厅基础与前沿项目 142300410038; 河南省教育厅基金资助项目 17A310006; 第59批国家博士后基金面上资助项目 2016M592314
#通信作者,男,1960年3月生,博士,教授,研究方向:药物化学,E-mail:liuhm@zzu.edu.cn
更新日期/Last Update: 2017-01-20