[1]吴诗文),张自森),刘 谦),等.二甲双胍联合化疗药物对人胃癌AGS细胞的作用*[J].郑州大学学报(医学版),2017,(01):37-41.[doi:10.13705/j.issn.1671-6825.2017.01.010]
 WU Shiwen),ZHANG Zisen),LIU Qian),et al.Effect of metformin combined with chemotherapeutic agents on gastric cancer cell line AGS[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(01):37-41.[doi:10.13705/j.issn.1671-6825.2017.01.010]
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二甲双胍联合化疗药物对人胃癌AGS细胞的作用*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年01期
页码:
37-41
栏目:
应用研究
出版日期:
2017-01-15

文章信息/Info

Title:
Effect of metformin combined with chemotherapeutic agents on gastric cancer cell line AGS
作者:
吴诗文1)张自森2)刘 谦1)王世超1)司远方1)赵胜男1)夏兴洲1)#
1)郑州大学第五附属医院消化内科 郑州 450052;
2)郑州大学第五附属医院肿瘤科 郑州 450052
Author(s):
WU Shiwen1) ZHANG Zisen2) LIU Qian1) WANG Shichao1) SI Yuanfang1) ZHAO Shengnan1) XIA Xingzhou1)
1)Department of Gastroenterology,the Fifth Affiliated Hospital,Zhengzhou University,Zhengzhou 450052;
2)Department of Oncology,the Fifth Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
二甲双胍 胃癌 顺铂 阿霉素 紫杉醇
Keywords:
metformin gastric cancer cisplatin doxorubicin paclitaxel
分类号:
R735.2
DOI:
10.13705/j.issn.1671-6825.2017.01.010
摘要:
目的:探讨二甲双胍(Met)联合化疗药物对人胃癌AGS细胞的影响。方法:应用Met及其分别联合顺铂(DDP)、阿霉素(ADM)、紫杉醇(PTX)处理人胃癌AGS细胞,应用CCK-8、Transwell模型、流式细胞术检测细胞增殖、迁移能力、侵袭能力及细胞凋亡。结果:Met可呈时间-剂量依赖性抑制AGS细胞增殖(P均<0.001),IC50为10 mmol/L。10 mmol/L Met、2 mg/L的DDP、0.02 mg/L的ADM、0.02 mg/L的PTX单用均可降低AGS细胞的迁移、侵袭能力,促其凋亡(P均<0.05),Met可协同3种化疗药物促进AGS细胞凋亡(P<0.05)。结论:Met有望用于胃癌的辅助化疗。
Abstract:
Aim: To investigate the effect of metformin(Met)combined with chemotherapeutic agents on human gastric cancer cell line AGS.Methods: AGS cells were treated with Met alone or in combination of cisplatin(DDP), doxorubicin(ADM)or paclitaxel(PTX).CCK-8, Transwell, and flow cytometry were used to detect cell proliferation, migration, invasion and apoptosis.Results: Met could inhibit cell proliferation in a dose- and time-dependent manner(P<0.001), and IC50 was 10 mmol/L. 10 mmol/L Met,2 mg/L DDP,0.02 mg/L ADM,0.02 mg/L PTX separate application could decrease the migration, invasion capabilities and prompt apoptosis of AGS cells(P<0.05), and Met had synergistic effected when being used with DDP, ADM or PTX in cell apoptosis(P<0.05).Conclusion: Met might be used for adjuvant chemotherapy for gastric cancer.

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备注/Memo

备注/Memo:
*河南省医学科技攻关计划项目 201403126
#通信作者,男,1964年9月生,硕士,主任医师,研究方向:胃癌的临床基础研究,E-mail:Zhougxia@163.com
更新日期/Last Update: 2017-01-20