[1]靳 玫),刘晓利),唐超智),等.Toll样受体在大鼠大脑皮层发育过程中的表达*[J].郑州大学学报(医学版),2017,(01):41-44.[doi:10.13705/j.issn.1671-6825.2017.01.011]
 JIN Mei),LIU Xiaoli),TANG Chaozhi),et al.Expressions of Toll-like receptors in development of rat cerebral cortex[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(01):41-44.[doi:10.13705/j.issn.1671-6825.2017.01.011]
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Toll样受体在大鼠大脑皮层发育过程中的表达*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年01期
页码:
41-44
栏目:
应用研究
出版日期:
2017-01-15

文章信息/Info

Title:
Expressions of Toll-like receptors in development of rat cerebral cortex
作者:
靳 玫1)刘晓利2)唐超智2)宿俊杰2)崔俊伟3)王文晟3)#
1)新乡医学院第三附属医院神经内科 河南新乡 453003;
2)河南师范大学生命科学学院 河南新乡 453007;
3)新乡医学院第一附属医院结核病研究所 河南卫辉 453100
Author(s):
JIN Mei1)LIU Xiaoli2)TANG Chaozhi2)SU Junjie2)CUI Junwei3)WANG Wensheng3)
1)Department of Neurology, the Third Affiliated Hospital, Xinxiang Medical University, Xinxiang,Henan 453003;
2)College of Life Sciences, Henan Normal University, Xinxiang, Henan 453007;
3)Tuberculosis Research Institute,the First Affiliated Hospital, Xinxiang Medical University, Weihui, Henan 453100
关键词:
TLR 大脑皮层 大鼠 大脑发育
Keywords:
Toll-like receptor cerebral cortex rat cerebral development
分类号:
R741.02
DOI:
10.13705/j.issn.1671-6825.2017.01.011
摘要:
目的:观察Toll样受体(TLR)在大鼠大脑分化和成熟过程中表达的变化。方法:取妊娠16、18 d的胎鼠和新生鼠(n=10)的大脑皮层,用抗体荧光染色检测Ki-67 和NeuN阳性细胞百分比,同时用实时荧光定量PCR和Western blot法检测TLR的表达。结果:随发育的进展,大鼠大脑皮层中 NeuN和Ki-67阳性细胞百分比逐渐降低(P<0.05),TLR1、TLR2、TLR3、TLR4、TLR7和TLR9的表达逐渐升高(P<0.05),而TLR5、TLR6和TLR8的表达无明显变化(P>0.05)。结论:TLR可能参与了神经前体细胞的分化和成熟。
Abstract:
Aim: To observe the changes of Toll-like receptor(TLR)expression in the differentiation and maturation of the rat brain.Methods: Cerebral cortex of fetal rats of 16 and 18 days and neonatal rats(n=10)were collected to detect Ki-67 and NeuN by fluorescent antibody, TLR expression by real-time PCR and Western blot method.Results: With the development of the progress, the percentages of NeuN positive cells and Ki-67 positive cells in the rat cerebral cortex decreased gradually(P<0.05), the expressions of TLR1, TLR2, TLR3, TLR4, TLR7 and TLR9 increased gradually(P<0.05), while the expressions of TLR5, TLR6 and TLR8 had no significant changes(P>0.05).Conclusion: TLR may be involved in the differentiation and maturation of neural progenitor cells.

参考文献/References:

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备注/Memo

备注/Memo:
*国家自然科学基金面上项目 31170733
#通信作者,男,1968年2月生,博士,教授,研究方向:神经性疾病,E-mail:2590212765@qq.com
更新日期/Last Update: 2017-01-20