[1]李玉山△,杨夕阳,侯方圆,等.特发性弱精子症患者精子中SEPT7的表达*[J].郑州大学学报(医学版),2017,(01):48-51.[doi:10.13705/j.issn.1671-6825.2017.01.013]
 LI Yushan,YANG Xiyang,HOU Fangyuan,et al.Expression of SEPT7 in sperms from patients with idiopathic asthenozoospermia[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(01):48-51.[doi:10.13705/j.issn.1671-6825.2017.01.013]
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特发性弱精子症患者精子中SEPT7的表达*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年01期
页码:
48-51
栏目:
应用研究
出版日期:
2017-01-15

文章信息/Info

Title:
Expression of SEPT7 in sperms from patients with idiopathic asthenozoospermia
作者:
李玉山杨夕阳侯方圆王全先刘军杰王琳凯苏彦华孙 琳
郑州大学第三附属医院人类精子库 郑州 450052
Author(s):
LI YushanYANG Xiyang HOU Fangyuan WANG Quanxian LIU JunjieWANG Linkai SU Yanhua SUN Lin
Human Sperm Bank,the Third Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
SEPT7 特发性弱精子症
Keywords:
Keywords SEPT7 idiopathic asthenozoospermia
分类号:
R698.2
DOI:
10.13705/j.issn.1671-6825.2017.01.013
摘要:
目的:检测特发性弱精子症患者精子中SEPT7的表达。方法:正常对照组30例,特发性弱精子症组30例(弱精子症组),采用密度梯度离心法收集精子,分别采用RT-PCR和Western blot检测精子中SEPT7 mRNA和蛋白的表达。结果:与正常对照组相比,弱精子症组精子中SEPT7 mRNA和蛋白的表达水平均降低(P均<0.001)。结论:SEPT7的表达水平降低可能影响精子活力。
Abstract:
Aim: To investigate the expression of SEPT7 in sperms from patients with idiopathic asthenozoospermia.Methods: Thirty patients with idiopathic asthenozoospermia and 30 normal controls were collected.The sperm were collected by density gradient centrifugation, and the expression levels of SEPT7 mRNA and protein were determined by RT-PCR and Western blot,respectively.Results: The expression levels of SEPT7 mRNA and protein in idiopathic asthenozoospermia group were obviously lower than those of normal control group(P<0.001).Conclusion: The decreased expression of SEPT7 may affect sperm motility.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省医学科技攻关项目 201503116
△男,1966年1月生,硕士,教授,研究方向:生殖免疫学,E-mail:liyushanrun@126.com
更新日期/Last Update: 2017-01-20