[1]杜燕华),李 懿),程宁宁),等.新布尼亚病毒IgG抗体间接ELISA检测方法的建立*[J].郑州大学学报(医学版),2017,(02):126-129.[doi:10.13705/j.issn.1671-6825.2017.02.005]
 DU Yanhua),LI Yi),CHENG Ningning),et al.Establishment and application of indirect ELISA of IgG antibody against new bunyavirus[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(02):126-129.[doi:10.13705/j.issn.1671-6825.2017.02.005]
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新布尼亚病毒IgG抗体间接ELISA检测方法的建立*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年02期
页码:
126-129
栏目:
论著
出版日期:
2017-03-15

文章信息/Info

Title:
Establishment and application of indirect ELISA of IgG antibody against new bunyavirus
作者:
杜燕华1)李 懿1)程宁宁2)许汴利1)黄学勇1)#
1)河南省疾病预防控制中心河南省医学病原生物学重点实验室 郑州 450016
2)郑州大学公共卫生学院流行病学教研室 郑州 450001
Author(s):
DU Yanhua1) LI Yi1)CHENG Ningning2)XU Bianli1) HUANG Xueyong1)
1)Henan Key Laboratory of Pathogenic Microorganisms,Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016
2)Department of Epidemiology,College of Public Health,Zhengzhou University,Zhengzhou 450001
关键词:
新布尼亚病毒 发热伴血小板减少综合征 间接ELISA法
Keywords:
new bunyavirus fever thrombocytopenia and leukopenia syndrome indirect ELISA
分类号:
R373.23
DOI:
10.13705/j.issn.1671-6825.2017.02.005
摘要:
目的:建立基于间接ELISA法检测新布尼亚病毒IgG抗体的血清学检测方法。方法:应用基因工程原核表达的新布尼亚病毒核蛋白,初步建立用于新布尼亚病毒IgG抗体检测的间接ELISA法,用该方法对267例发热伴血小板减少综合征患者急性期和恢复期双份血清和198份标本进行检测,并与间接免疫荧光法的结果进行对比。结果:所建立的新布尼亚病毒IgG抗体间接ELISA检测方法重复性好,与其他病毒无交叉反应。与间接免疫法对267例发热伴血小板减少综合征病例的实验室诊断结果有较好的一致性(Kappa=0.69),健康人血清检测阳性率为5.56%。结论:建立的间接ELISA方法适用于新布尼亚病毒感染的实验室诊断及流行病学调查。
Abstract:
Aim: To establish an indirect ELISA method for detecting IgG antibody against new bunyavirus.Methods: Based on nucleoprotein gene of new bunyavirus obtained by prokaryotic expression, a qualitative indirect ELISA method for detecting IgG antibody was set up. Using the established method,serum samples from both acute and recovery phases of 267 patients with fever thrombocytopenia and leukopenia syndrome and 198 healthy persons were tested.The results were compared with IFA.Results: The established indirect ELISA method had better repeatability, no cross reaction with other virus. There was no significant difference in results between IFA and indirect ELISA in 267 patients with fever thrombocytopenia and leukopenia syndrome(Kappa=0.69),and the positive rate in the healthy was 5.56%.Conclusion: The indirect ELISA method for detecting IgG antibody against new bunyavirus has been established successfully, which is suitable for laboratory diagnosis and epidemiological investigation for fever thrombocytopenia and leukopenia syndrome.

参考文献/References:

[1] 许汴利.新布尼亚病毒感染致发热伴血小板减少综合征的发现、认识与启示[J].中华预防医学杂志,2012,46(2):99
[2] XU B,LIU L,HUANG X,et al.Metagenomic analysis of fever, thrombocytopenia and leukopenia syndrome(FTLS)in Henan Province, China: discovery of a new bunyavirus[J].PLoS Pathog,2011,7(11):e1002369
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备注/Memo

备注/Memo:
*国家自然科学基金项目 81573204; 河南省医学科技攻关计划项目 201303194
#通信作者,男,1969年10月生,博士,主任医师,研究方向:分子流行病学,E-mail:huangxy@hncdc.com.cn
更新日期/Last Update: 2017-03-20